Eif4e Mouse shRNA Plasmid (Locus ID 13684)

CAT#: TL510658

Eif4e - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided



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CNY 5,740.00


货期*
现货

规格
    • 1 kit

Product images

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Lenti-vpak packaging kit - packaging plasmids and transfection reagent
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TurboFectin Transfection Reagent (1 mL in 1 vial)
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Lateral flow testing sticks used for the semi-quantitative detection of the lentiviral p24 protein, 20 tests
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Specifications

Product Data
Product Name Eif4e Mouse shRNA Plasmid (Locus ID 13684)
Locus ID 13684
UniProt ID P63073
Synonyms EG668879; eIF-4; eIF-4E; Eif4e-ps; If4; If4e
Vector pGFP-C-shLenti
Format Lentiviral plasmids
Kit Components Eif4e - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 13684). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq BC010759, BC085087, NM_007917, NM_007917.1, NM_007917.2, NM_007917.3, NM_007917.4, BC010759.1
Summary This gene encodes a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. It has also been associated with autism spectrum disorders. Consistently, knockout of this gene results in increased translation of neuroligins, postsynaptic proteins linked to autism spectrum disorders. Pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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