pGFP-C-shLenti shRNA Vector

CAT＃: TR30023

HuSH shRNA GFP Lenti Cloning Vector (pGFP-C-shLenti Vector), 5 ug

Datasheet
SDS
Vector Sequence

CNY 4,090.00

货期*
现货
规格
    • 5 ug
Cited in 104 publications.

Product images

Specifications

Product Data
Control Type Negative
Fluorescent Label TurboGFP
Viral Packaging Lentiviral
Feature
  • pGFP-C-shLenti vector is a third generation Lentivector which requires the viral components carried in other vectors to produce viral particles. There are three major functional elements within the 5LTR and 3LTR: a shRNA expression cassette driven by an U6 promoter, a puromycin resistant gene driven by a SV40 promoter and a tGFP gene driven by a CMV promoter. All of them can be packaged to viral particles and transduced to many cell lines. The bacterial selection marker for the vector is Chloramphenicol.
  • U6_F Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (104)

The use of this Vectors has been cited in the following citations:
A nuclear NKRF interacting long noncoding RNA controls EBV eradication and suppresses tumor progression in natural killer/T-cell lymphoma. ,null, Biochimica et biophysica acta. Molecular basis of disease ,PubMed ID 37084822
Role of circular RNA cdr1as in modulation of macrophage phenotype ,null, Life sciences ,PubMed ID 36181865
A Cdh3-β-catenin-laminin signaling axis in a subset of breast tumor leader cells control leader cell polarization and directional collective migration ,null, Developmental cell ,PubMed ID 36626870
NCK-associated protein 1 regulates metastasis and is a novel prognostic marker for colorectal cancer. ,null, Cell death discovery ,PubMed ID 36639705
Mitofusin 2 Integrates Mitochondrial Network Remodelling, Mitophagy and Renewal of Respiratory Chain Proteins in Neurons after Oxygen and Glucose Deprivation. ,null, Molecular neurobiology ,PubMed ID 35962299
Alternative splicing of Apoptosis Stimulating Protein of TP53-2 (ASPP2) results in an oncogenic isoform promoting migration and therapy resistance in soft tissue sarcoma (STS) ,null, BMC Cancer ,PubMed ID 35780096
Neural network learning defines glioblastoma features to be of neural crest perivascular or radial glia lineages ,null, Science Advances ,PubMed ID 35675414
Senecavirus a 3D Interacts with NLRP3 to Induce IL-1β Production by Activating NF-κB and Ion Channel Signals ,null, Microbiology Spectrum ,PubMed ID 35254168
Temporal profiling of therapy resistance in human medulloblastoma identifies novel targetable drivers of recurrence ,null, Science Advances ,PubMed ID 34878832
Depletion of NK6 Homeobox 3 (NKX6.3) causes gastric carcinogenesis through copy number alterations by inducing impairment of DNA replication and repair regulation ,null, Oncogenesis ,PubMed ID 34893582
FMDV Leader Protein Interacts with the NACHT and LRR Domains of NLRP3 to Promote IL-1β Production ,null, Viruses ,PubMed ID 35062226
ASPP2κ Is Expressed In Human Colorectal Carcinoma And Promotes Chemotherapy Resistance And Tumorigenesis ,null, Frontiers in Molecular Biosciences ,PubMed ID 34805267
Loss of SNORA73 reprograms cellular metabolism and protects against steatohepatitis ,null, Nature Communications ,PubMed ID 34471131
lncRNA ST8SIA6-AS1 facilitates proliferation and invasion in liver cancer by regulating miR-142-3p ,null, Experimental and Therapeutic Medicine ,PubMed ID 34659494
CREB3L2 Modulates Nerve Growth Factor-Induced Cell Differentiation ,null, Frontiers in Molecular Neuroscience ,PubMed ID 34421533
Dipeptidyl peptidase 4 contributes to Alzheimer’s disease–like defects in a mouse model and is increased in sporadic Alzheimer’s disease brains ,null, The Journal of Biological Chemistry ,PubMed ID 34265307
SKAP2 as a new regulator of oligodendroglial migration and myelin sheath formation ,null, Glia ,PubMed ID 34324225
Intrinsic OXPHOS limitations underlie cellular bioenergetics in leukemia ,null, eLife ,PubMed ID 34132194
Aminopeptidase A contributes to biochemical, anatomical and cognitive defects in Alzheimer’s disease (AD) mouse model and is increased at early stage in sporadic AD brain ,null, Acta Neuropathologica ,PubMed ID 33881611
FGF23 ameliorates ischemia-reperfusion induced acute kidney injury via modulation of endothelial progenitor cells: targeting SDF-1/CXCR4 signaling ,Chang, HM;Peng, KY;Chan, CK;Sun, CY;Chen, YY;Chang, HM;Huang, CL;Liu, PC;Chen, PY;Wang, KC;Wang, WJ;Wu, CC;Lin, YF;Lai, TS;Huang, TM;Young, GH;Lin, SL;Ostermann, M;Chu, TS;Chueh, JS;Wu, VC;, Cell death & disease ,PubMed ID 33866326 [pGFP-C-shLenti]
c-MYC-directed NRF2 drives malignant progression of head and neck cancer via glucose-6-phosphate dehydrogenase and transketolase activation ,null, Theranostics ,PubMed ID 33859744
O-GlcNAcylation Enhances NUSAP1 Stability and Promotes Bladder Cancer Aggressiveness ,Chen, Y;Liu, J;Zhang, W;Kadier, A;Wang, R;Zhang, H;Yao, X;, OncoTargets and therapy ,PubMed ID 33488099 [pGFP-C-shLenti]
LINC02418 promotes colon cancer progression by suppressing apoptosis via interaction with miR-34b-5p/BCL2 axis ,Tian, J;Cui, P;Li, Y;Yao, X;Wu, X;Wang, Z;Li, C;, Cancer cell international ,PubMed ID 32973404 [pGFP-C-shLenti]
Hypoxia-induced increase in Sug1 leads to poor post-transplantation survival of allogeneic mesenchymal stem cells. ,null, FASEB journal : official publication of the Federation of American Societies for Experimental Biology ,PubMed ID 32770803
Ribosome Collisions Trigger General Stress Responses to Regulate Cell Fate ,Wu, CC;Peterson, A;Zinshteyn, B;Regot, S;Green, R;, Cell ,PubMed ID 32610081 [pGFP-C-shLenti]
Metformin inhibits IL-6 signaling by decreasing IL-6R expression on multiple myeloma cells. ,null, Leukemia ,PubMed ID 30988378
Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development ,Bikbaev, A;Ciuraszkiewicz-Wojciech, A;Heck, J;Klatt, O;Freund, R;Mitlöhner, J;Enrile Lacalle, S;Sun, M;Repetto, D;Frischknecht, R;Ablinger, C;Rohlmann, A;Missler, M;Obermair, GJ;Di Biase, V;Heine, M;, J. Neurosci. ,PubMed ID 32414783 [pGFP-C-shLenti]
Norrin mediates tumor-promoting and -suppressive effects in glioblastoma via Notch and Wnt ,null, The Journal of Clinical Investigation ,PubMed ID 32182224
Long noncoding RNA lncARSR confers resistance to Adriamycin and promotes osteosarcoma progression ,null, Cell Death & Disease ,PubMed ID 32404870
Mir-370-3p Impairs Glioblastoma Stem-Like Cell Malignancy Regulating a Complex Interplay between HMGA2/HIF1A and the Oncogenic Long Non-Coding RNA (lncRNA) NEAT1 ,Lulli, V;Buccarelli, M;Ilari, R;Castellani, G;De Dominicis, C;Di Giamberardino, A;D Alessandris, QG;Giannetti, S;Martini, M;Stumpo, V;Boe, A;De Luca, G;Biffoni, M;Marziali, G;Pallini, R;Ricci-Vitiani, L;, Int J Mol Sci ,PubMed ID 32443824 [pGFP-C-shLenti]
PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathway. ,null, Carcinogenesis ,PubMed ID 31095674
LINC02476 Promotes the Malignant Phenotype of Hepatocellular Carcinoma by Sponging miR-497 and Increasing HMGA2 Expression ,Duan, Y;Zhao, M;Jiang, M;Li, Z;Ni, C;, Onco Targets Ther ,PubMed ID 32280244 [pGFP-C-shLenti]
Serine Biosynthesis Pathway Supports MYC–miR-494–EZH2 Feed-Forward Circuit Necessary to Maintain Metabolic and Epigenetic Reprogramming of Burkitt Lymphoma Cells ,null, Cancers ,PubMed ID 32138178
Casticin elicits inflammasome-induced pyroptosis through activating PKR/JNK/NF-κB signal in 5-8F cells ,Jiang, C;Shi, R;Chen, B;Yan, X;Tang, G;, Biomed. Pharmacother. ,PubMed ID 31951974 [pGFP-C-shLenti]
Alpha-Catulin, a New Player in a Rho Dependent Apical Constriction That Contributes to the Mouse Neural Tube Closure ,Karpińska, K;Cao, C;Yamamoto, V;Gielata, M;Kobielak, A;, Front. Cell Dev. Biol. ,PubMed ID 32258033 [pGFP-C-shLenti]
Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain ,null, The Journal of Cell Biology ,PubMed ID 31910261
Effects of Galectin-1 on Biological Behavior in Cervical Cancer ,null, Journal of Cancer ,PubMed ID 32047564
Synthetically Lethal Interactions of Heme Oxygenase-1 and Fumarate Hydratase Genes ,null, Biomolecules ,PubMed ID 31963199
Identification of a LIF-Responsive, Replication-Competent Subpopulation of Human β Cells ,Rosado-Olivieri, EA;Aigha, II;Kenty, JH;Melton, DA;, Cell Metab. ,PubMed ID 31928884 [pGFP-C-shLenti]
Endocytosis of very low-density lipoproteins: an unexpected mechanism for lipid acquisition by breast cancer cells ,Lupien, LE;Bloch, K;Dehairs, J;Traphagen, NA;Feng, WW;Dennis, T;Davis, WL;Swinnen, JV;Wells, WA;Smits, NC;Kuemmerle, NB;Miller, TW;Kinlaw, WB;, J. Lipid Res. ,PubMed ID 31806729 [pGFP-C-shLenti]
Oncogenic role of TYRO3 receptor tyrosine kinase in the progression of pancreatic cancer. ,null, Cancer letters ,PubMed ID 31765735
Metabolic heterogeneity confers differences in melanoma metastatic potential ,null, Nature ,PubMed ID 31853067
BC200 overexpression contributes to luminal and triple negative breast cancer pathogenesis ,Barton, M;Santucci-Pereira, J;Vaccaro, OG;Nguyen, T;Su, Y;Russo, J;, BMC Cancer ,PubMed ID 31646972 [pGFP-C-shLenti]
Interaction of tumor cells and astrocytes promotes breast cancer brain metastases through TGF-β2/ANGPTL4 axes ,null, NPJ Precision Oncology ,PubMed ID 31602400
Ankyrin-G induces nucleoporin Nup358 to associate with the axon initial segment of neurons. ,null, Journal of cell science ,PubMed ID 31427429
Glycolipids Recognized by A2B5 Antibody Promote Proliferation, Migration, and Clonogenicity in Glioblastoma Cells ,null, Cancers ,PubMed ID 31466399
Argonaute 4 as an Effector Protein in RNA-Directed DNA Methylation in Human Cells ,Chalertpet, K;Pin-On, P;Aporntewan, C;Patchsung, M;Ingrungruanglert, P;Israsena, N;Mutirangura, A;, Front Genet ,PubMed ID 31333722 [pGFP-C-shLenti]
TNF-α/IL-1β-licensed mesenchymal stromal cells promote corneal allograft survival via myeloid cell-mediated induction of Foxp3+ regulatory T cells in the lung. ,null, FASEB journal : official publication of the Federation of American Societies for Experimental Biology ,PubMed ID 31108041
AIF-regulated oxidative phosphorylation supports lung cancer development ,null, Cell Research ,PubMed ID 31133695
YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing β cells ,null, Nature Communications ,PubMed ID 30931946
Melatonin protects chronic kidney disease mesenchymal stem cells against senescence via PrPC dependent enhancement of the mitochondrial function ,Han, YS;Kim, S;Lee, JH;Jung, SK;Noh, H;Lee, SH;, J. Pineal Res. ,PubMed ID 30372554 [pGFP-C-shLenti]
A conserved HH-Gli1-Mycn network regulates heart regeneration from newt to human ,Singh, BN;Koyano-Nakagawa, N;Gong, W;Moskowitz, IP;Weaver, CV;Braunlin, E;Das, S;van Berlo, JH;Garry, MG;Garry, DJ;, Nat Commun ,PubMed ID 30315164 [pGFP-C-shLenti]
Pharmacological versus genetic inhibition of heme oxygenase-1 - the comparison of metalloporphyrins, shRNA and CRISPR/Cas9 system. ,null, Acta biochimica Polonica ,PubMed ID 29694447
S100A9 regulates cisplatin chemosensitivity of squamous cervical cancer cells and related mechanism ,null, Cancer Management and Research ,PubMed ID 30288106
Intrathecal Injection of Dual Zipper Kinase shRNA Alleviating the Neuropathic Pain in a Chronic Constrictive Nerve Injury Model ,null, International Journal of Molecular Sciences ,PubMed ID 30115872
LINC01287 regulates tumorigenesis and invasion via miR-298/MYB in hepatocellular carcinoma ,Mo, Y;He, L;Lai, Z;Wan, Z;Chen, Q;Pan, S;Li, L;Li, D;Huang, J;Xue, F;Che, S;, J. Cell. Mol. Med. ,PubMed ID 30133116 [pGFP-C-shLenti]
Arsenic Promotes the COX2/PGE2-SOX2 Axis to Increase the Malignant Stemness Properties of Urothelial Cells ,null, International journal of cancer ,PubMed ID 29396848
LINC01287/miR-298/STAT3 feedback loop regulates growth and the epithelial-to-mesenchymal transition phenotype in hepatocellular carcinoma cells ,Mo, Y;He, L;Lai, Z;Wan, Z;Chen, Q;Pan, S;Li, L;Li, D;Huang, J;Xue, F;Che, S;, J. Exp. Clin. Cancer Res. ,PubMed ID 30001751 [pGFP-C-shLenti]
Breast cancer stem cells characterized by CD70 expression preferentially metastasize to the lungs. ,null, Breast cancer (Tokyo, Japan) ,PubMed ID 29948958
LncRNA ZEB2-AS1 promotes pancreatic cancer cell growth and invasion through regulating the miR-204/HMGB1 axis. ,null, International journal of biological macromolecules ,PubMed ID 29753015
Attenuation of EGFL7 Expression Inhibits Growth Hormone-Producing Pituitary Adenomas Growth and Invasion. ,null, Human gene therapy ,PubMed ID 29466872
miR-126 suppresses epithelial-to-mesenchymal transition and metastasis by targeting PI3K/AKT/Snail signaling of lung cancer cells ,Jia, Z;Zhang, Y;Xu, Q;Guo, W;Guo, A;, Oncol Lett ,PubMed ID 29725450 [pGFP-C-shLenti]
Meox1 accelerates myocardial hypertrophic decompensation through Gata4. ,null, Cardiovascular research ,PubMed ID 29155983
The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology ,null, The Journal of Biological Chemistry ,PubMed ID 29269412
Interleukin-30/IL-27p28 shapes prostate cancer stem-like cell behavior and is critical for tumor onset and metastasization ,Sorrentino, C;Ciummo, SL;Cipollone, G;Caputo, S;Bellone, M;Di Carlo, E;, Cancer Res. ,PubMed ID 29487200 [pGFP-C-shLenti]
Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery ,null, eLife ,PubMed ID 29271742
CREB3L1-mediated functional and structural adaptation of the secretory pathway in hormone-stimulated thyroid cells ,null, Journal of Cell Science ,PubMed ID 29093023
YAP1 and COX2 coordinately regulate urothelial cancer stem-like cells ,null, Cancer research ,PubMed ID 29180467
RBR-type E3 ubiquitin ligase RNF144A targets PARP1 for ubiquitin-dependent degradation and regulates PARP inhibitor sensitivity in breast cancer cells ,null, Oncotarget ,PubMed ID 29212245
The abundance of metabolites related to protein methylation correlates with the metastatic capacity of human melanoma xenografts ,null, Science Advances ,PubMed ID 29109980
Global metabolic profiling identifies a pivotal role of proline and hydroxyproline metabolism in supporting hypoxic response in hepatocellular carcinoma ,Tang, L;Zeng, J;Geng, P;Fang, C;Wang, Y;Sun, M;Wang, C;Wang, J;Yin, P;Hu, C;Guo, L;Yu, JJ;Gao, P;Li, E;Zhuang, Z;Xu, G;Liu, Y;, Clin. Cancer Res.2017 ,PubMed ID 29084919 [pGFP-C-shLenti]
The interaction between HIV-1 Nef and adaptor protein-2 reduces Nef-mediated CD4+ T cell apoptosis ,null, Virology ,PubMed ID 28577469
Proinflammatory CXCL12-CXCR4/CXCR7 signaling axis drives Myc-induced prostate cancer in obese mice ,null, Cancer research ,PubMed ID 28687617
Blockade and knock-out of CALHM1 channels attenuate ischemic brain damage ,null, Journal of Cerebral Blood Flow & Metabolism ,PubMed ID 28597712
RGS12 is a novel tumor suppressor gene in African American prostate cancer that represses AKT and MNX1 expression ,null, Cancer research ,PubMed ID 28611045
Monocrotaline Induces Endothelial Injury and Pulmonary Hypertension by Targeting the Extracellular Calcium-Sensing Receptor ,Xiao, R;Su, Y;Feng, T;Sun, M;Liu, B;Zhang, J;Lu, Y;Li, J;Wang, T;Zhu, L;Hu, Q;, J Am Heart Assoc ,PubMed ID 28330842 [pGFP-C-shLenti]
PNPLA1 is a transacylase essential for the generation of the skin barrier lipid ω-O-acylceramide ,Ohno, Y;Kamiyama, N;Nakamichi, S;Kihara, A;, Nat Commun ,PubMed ID 28248318 [pGFP-C-shLenti]
Capsaicin-induced Ca2+ signaling is enhanced via upregulated TRPV1 channels in pulmonary artery smooth muscle cells from patients with idiopathic PAH ,null, American Journal of Physiology - Lung Cellular and Molecular Physiology ,PubMed ID 27979859
High Expression of Cell Division Cycle 42 Promotes Pancreatic Cancer Growth and Predicts Poor Outcome of Pancreatic Cancer Patients ,Yang, D;Zhang, Y;Cheng, Y;Hong, L;Wang, C;Wei, Z;Cai, Q;Yan, R;, Dig. Dis. Sci ,PubMed ID 28181096 [pGFP-C-shLenti]
Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination ,null, Nature Communications ,PubMed ID 28176781
The mitotic checkpoint regulator RAE1 induces aggressive breast cancer cell phenotypes by mediating epithelial-mesenchymal transition ,null, Scientific Reports ,PubMed ID 28181567
Aberrant corticosteroid metabolism in tumor cells enables GR takeover in enzalutamide resistant prostate cancer ,null, eLife ,PubMed ID 28191869
Regulation of cancerous progression and epithelial-mesenchymal transition by miR-34c-3p via modulation of MAP3K2 signaling in triple-negative breast cancer cells. ,null, Biochemical and biophysical research communications ,PubMed ID 28069384
Targeting tissue inhibitor of metalloproteinase 1/2 using a shRNA lentiviral system offers a novel treatment strategy against hepatic fibrosis ,Zhang, S;Liu, Q;Xiao, J;Lei, J;Chai, Y;Liu, Y;Hong, Z;, Int J Clin Exp Med [pGFP-C-shLenti]
Hyaluronan Production Regulates Metabolic and Cancer Stem-like Properties of Breast Cancer Cells via Hexosamine Biosynthetic Pathway-coupled HIF-1 Signaling * ,null, The Journal of Biological Chemistry ,PubMed ID 27758869
Hypoxia-induced expression of cellular prion protein improves the therapeutic potential of mesenchymal stem cells ,null, Cell Death & Disease ,PubMed ID 27711081
Platelet-derived growth factor-α receptor is the cellular receptor for human cytomegalovirus gHgLgO trimer ,null, Nature microbiology ,PubMed ID 27573107
Nkx6.1-mediated insulin secretion and β-cell proliferation is dependent on upregulation of c-Fos. ,null, FEBS letters ,PubMed ID 27164028
Mutual inhibition of insulin signaling and PHLPP-1 determines cardioprotective efficiency of Akt in aged heart ,Xing, Y;Sun, W;Wang, Y;Gao, F;Ma, H;, Aging (Albany NY) ,PubMed ID 27019292
G protein-coupled receptor GPR160 is associated with apoptosis and cell cycle arrest of prostate cancer cells ,null, Oncotarget ,PubMed ID 26871479
Pur-alpha regulates cytoplasmic stress granule dynamics and ameliorates FUS toxicity ,null, Acta neuropathologica ,PubMed ID 26728149
Expression and Function of Phosphodiesterase Type 5 in Human Breast Cancer Cell Lines and Tissues: Implications for Targeted Therapy. ,null, Clinical cancer research : an official journal of the American Association for Cancer Research ,PubMed ID 26667489
Nerve Growth Factor Regulates Transient Receptor Potential Vanilloid 2 via Extracellular Signal-Regulated Kinase Signaling To Enhance Neurite Outgrowth in Developing Neurons ,null, Molecular and Cellular Biology ,PubMed ID 26416880
Dipeptide species regulate p38MAPK–Smad3 signalling to maintain chronic myelogenous leukaemia stem cells ,null, Nature Communications ,PubMed ID 26289811
Microenvironmental interleukin-6 suppresses toll-like receptor signaling in human leukemia cells through miR-17/19A. ,null, Blood ,PubMed ID 26041742
ARID3B Directly Regulates Ovarian Cancer Promoting Genes ,null, PLoS ONE ,PubMed ID 26121572
α-Synuclein, a chemoattractant, directs microglial migration via H2O2-dependent Lyn phosphorylation ,null, Proceedings of the National Academy of Sciences of the United States of America ,PubMed ID 25825709
Acyl-CoA synthetase 1 deficiency alters cardiolipin species and impairs mitochondrial function ,null, Journal of Lipid Research ,PubMed ID 26136511
Protein composition and movements of membrane swellings associated with primary cilia ,Mohieldin, AM;Haymour, HS;Lo, ST;AbouAlaiwi, WA;Atkinson, KF;Ward, CJ;Gao, M;Wessely, O;Nauli, SM;, Cell. Mol. Life Sci. ,PubMed ID 25650235 [TR30023]
MicroRNA-141 and MicroRNA-146b-5p Inhibit the Prometastatic Mesenchymal Characteristics through the RNA-binding Protein AUF1 Targeting the Transcription Factor ZEB1 and the Protein Kinase AKT * ,null, The Journal of Biological Chemistry ,PubMed ID 25261470
Rapid neurogenesis through transcriptional activation in human stem cells ,null, Molecular Systems Biology ,PubMed ID 25403753
Ageing related periostin expression increase from cardiac fibroblasts promotes cardiomyocytes senescent. ,null, Biochemical and biophysical research communications ,PubMed ID 25173938
Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells ,null, Nature ,PubMed ID 25079315
Temozolomide down-regulates P-glycoprotein in human blood-brain barrier cells by disrupting Wnt3 signaling. ,null, Cellular and molecular life sciences : CMLS ,PubMed ID 23771630
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