WNK1 Human shRNA Plasmid Kit (Locus ID 65125)

Specifications

Product Data
Product Name	WNK1 Human shRNA Plasmid Kit (Locus ID 65125)
Locus ID	65125
UniProt ID	Q9H4A3
Synonyms	HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1; PSK
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	WNK1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 65125). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	BC021121, NM_001184985, NM_014823, NM_018979, NM_213655, NM_213655.3, NM_213655.4, NM_018979.1, NM_018979.2, NM_018979.3, NM_014823.2, NM_001184985.1, BC130467, BC013629, BC035146, BC044600, BC071959, BC094862, BC130469, BC141881, BC172444, BM716053, NM_014823.3, NM_018979.4
Summary	This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.