ICK Human shRNA Plasmid Kit (Locus ID 22858)
CAT#: TL320661
ICK - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
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CNY 7,740.00
货期*
2周
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Specifications
Product Data | |
Product Name | ICK Human shRNA Plasmid Kit (Locus ID 22858) |
Locus ID | 22858 |
UniProt ID | Q9UPZ9 |
Synonyms | ECO; EJM10; hICK; ICK; LCK2; MRK |
Vector | pGFP-C-shLenti |
Format | Lentiviral plasmids |
Kit Components | ICK - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 22858). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free. |
RefSeq | BC035807, NM_014920, NM_016513, NM_173041, NM_014920.1, NM_014920.2, NM_014920.3, NM_016513.1, NM_016513.2, NM_016513.3, NM_016513.4, BC035807.1, BC136420, BC136421, BC152464, NM_014920.5, NM_016513.5 |
Summary | Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. [provided by RefSeq, Jul 2008] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
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