CD8B Human shRNA Plasmid Kit (Locus ID 926)

Specifications

Product Data
Product Name	CD8B Human shRNA Plasmid Kit (Locus ID 926)
Locus ID	926
UniProt ID	P10966
Synonyms	CD8B1; LEU2; LY3; LYT3; P37
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	CD8B1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 926). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001178100, NM_004931, NM_172099, NM_172100, NM_172101, NM_172102, NM_172213, NM_172101.1, NM_172101.2, NM_172101.3, NM_172213.1, NM_172213.2, NM_172213.3, NM_004931.1, NM_004931.2, NM_004931.3, NM_004931.4, NM_172102.1, NM_172102.2, NM_172102.3, NM_001178100.1, NM_172099.1, NM_172099.2, BC100911, BC100912, BC100913, BC100914, NM_172102.4, NM_172101.4, NM_004931.5
Summary	The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.