RPL3 Human shRNA Plasmid Kit (Locus ID 6122)
CAT#: TL309744
RPL3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
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CNY 7,740.00
货期*
2周
规格
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Specifications
Product Data | |
Product Name | RPL3 Human shRNA Plasmid Kit (Locus ID 6122) |
Locus ID | 6122 |
UniProt ID | P39023 |
Synonyms | ASC-1; L3; TARBP-B |
Vector | pGFP-C-shLenti |
Format | Lentiviral plasmids |
Kit Components | RPL3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 6122). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free. |
RefSeq | NM_000967, NM_001033853, NM_000967.1, NM_000967.2, NM_000967.3, NM_001033853.1, BC002408, BC002408.2, BC008492, BC008492.1, BC004323, BC006483, BC008003, BC012146, BC012786, BC013674, BC013861, BC014017, BC015032, BC015767, BC022790, BC033296, BC036582, BC063662, BC071666, BC088373, BC107711, BC127794, NM_001033853.2, NM_000967.4 |
Summary | Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene's introns. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
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