ENSA Human shRNA Plasmid Kit (Locus ID 2029)

Specifications

Product Data
Product Name	ENSA Human shRNA Plasmid Kit (Locus ID 2029)
Locus ID	2029
UniProt ID	O43768
Synonyms	ARPP-19e
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	ENSA - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 2029). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_004436, NM_207042, NM_207043, NM_207044, NM_207045, NM_207046, NM_207047, NM_207168, NM_004436.1, NM_004436.2, NM_207168.1, NM_207044.1, NM_207043.1, NM_207047.1, NM_207042.1, NM_207045.1, NM_207046.1, BC004461, BC004461.1, BC069208, BC069208.1, BC068544, BC068544.1, BC000436, BM771663, BM984677, BM993811, NM_004436.4, NM_207046.2, NM_207045.2, NM_207042.2
Summary	The protein encoded by this gene belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes. At least eight alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.