Growth Hormone (GH1) Human shRNA Plasmid Kit (Locus ID 2688)

Specifications

Product Data
Product Name	Growth Hormone (GH1) Human shRNA Plasmid Kit (Locus ID 2688)
Locus ID	2688
UniProt ID	P01241
Synonyms	GH; GH-N; GHB5; GHN; hGH-N; IGHD1A; IGHD1B; IGHD2
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	GH1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 2688). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_000515, NM_022559, NM_022560, NM_022561, NM_022562, NM_022559.2, NM_022559.3, NM_000515.1, NM_000515.2, NM_000515.3, NM_000515.4, NM_022561.1, NM_022561.2, NM_022560.1, NM_022560.2, NM_022560.3, NM_022562.1, NM_022562.2, BC062475, BC062475.1, BC090045, BC075012, BC075013, NM_000515.5, NM_022559.4, NM_022560.4
Summary	The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq, Jul 2008]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.