Prion protein PrP (PRNP) Human shRNA Plasmid Kit (Locus ID 5621)

Specifications

Product Data
Product Name	Prion protein PrP (PRNP) Human shRNA Plasmid Kit (Locus ID 5621)
Locus ID	5621
UniProt ID	P04156
Synonyms	AltPrP; ASCR; CD230; CJD; GSS; KURU; p27-30; PRIP; PrP; PrP27-30; PrP33-35C; PrPc
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	PRNP - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 5621). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_000311, NM_001080121, NM_001080122, NM_001080123, NM_001271561, NM_183079, NM_001080123.1, NM_001080123.2, NM_000311.1, NM_000311.2, NM_000311.3, NM_000311.4, NM_183079.1, NM_183079.2, NM_183079.3, NM_001080122.1, NM_001080122.2, NM_001080121.1, NM_001080121.2, NM_001271561.1, NM_001271561.2, BC012844, BC012844.1, BC022532, BC022532.1, BC016809, NM_001271561.3, NM_183079.4, NM_001080122.3, NM_001080121.3, NM_001080123.3, NM_000311.5
Summary	The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.