SKP2 Human shRNA Plasmid Kit (Locus ID 6502)
CAT#: TL301684
SKP2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
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CNY 5,740.00
货期*
现货
规格
Cited in 1 publication. |
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Specifications
Product Data | |
Product Name | SKP2 Human shRNA Plasmid Kit (Locus ID 6502) |
Locus ID | 6502 |
UniProt ID | Q13309 |
Synonyms | FBL1; FBXL1; FLB1; p45 |
Vector | pGFP-C-shLenti |
Format | Lentiviral plasmids |
Kit Components | SKP2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 6502). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free. |
RefSeq | NM_001243120, NM_005983, NM_032637, NM_032637.1, NM_032637.2, NM_032637.3, NM_005983.1, NM_005983.2, NM_005983.3, NM_001243120.1, BC001441, BC001441.2, BC007441, NM_005983.4, NM_032637.4 |
Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2011] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (1)
The use of this RNAi has been cited in the following citations: |
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OCT-4: a novel estrogen receptor-a collaborator that promotes tamoxifen resistance in breast cancer cells
,Bhatt, S;Stender, JD;Joshi, S;Wu, G;Katzenellenbogen, BS;,
Oncogene
,PubMed ID 27065334
[SKP2]
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