ANO6 Human shRNA Plasmid Kit (Locus ID 196527)

Specifications

Product Data
Product Name	ANO6 Human shRNA Plasmid Kit (Locus ID 196527)
Locus ID	196527
UniProt ID	Q4KMQ2
Synonyms	BDPLT7; SCTS; TMEM16F
Vector	pRFP-C-RS
Format	Retroviral plasmids
Kit Components	DKFZp313M0720 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 196527). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq	NM_001025356, NM_001142678, NM_001142679, NM_001142680, NM_001204803, NM_001025356.1, NM_001025356.2, NM_001142678.1, NM_001142679.1, NM_001142680.1, NM_001204803.1, BC098410, BC098410.1, BC042383, BC063576, BC073891, BC136445, NM_001142679.2, NM_001204803.2, NM_001025356.3, NM_001142678.2
Summary	This gene encodes a multi-pass transmembrane protein that belongs to the anoctamin family. This protein is an essential component for the calcium-dependent exposure of phosphatidylserine on the cell surface. The scrambling of phospholipid occurs in various biological systems, such as when blood platelets are activated, they expose phosphatidylserine to trigger the clotting system. Mutations in this gene are associated with Scott syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.