LIF (NM_002309) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) A DNA sequence encoding the region (Ser23-Phe202) of human LIF
Tag	Tag Free
Predicted MW	19.8 kDa
Concentration	Resuspend the protein to the desired concentration in proper buffer.
Purity	>95% as determined by SDS-PAGE and Coomassie blue staining
Buffer	Lyophilized from a sterile solution containing 20 mM PB, pH 7.4
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity	Determined by its ability to induce differentiation of M1 cells. ED50 for this effect is typically < 0.04 ng/ml.
Endotoxin	< 0.1 EU per 1 µg of the protein by the LAL
Reference Data
RefSeq	NP_002300
Locus ID	3976
UniProt ID	P15018
Refseq Size	3987
Cytogenetics	22q12.2
Refseq ORF	606
Synonyms	CDF; DIA; HILDA; MLPLI
Summary	The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
Protein Families	Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.