LIF (NM_002309) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Ser23-Phe202
Tag	Tag Free
Predicted MW	19.8 kDa
Concentration	lot specific
Purity	>95% as determined by SDS-PAGE and Coomassie blue staining
Buffer	Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 0.02% Tween 20, pH 7.4
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-5 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Stability	Stable for at least 6 months from date of receipt under proper storage and handling conditions.
Bioactivity	ED50 is less than 0.01 ng/ml as determined by the M1 cell differentiation assay. Specific Activity of 1.0 x 108 IU/mg.
Endotoxin	< 0.1 EU per µg protein as determined by LAL test
Reference Data
RefSeq	NP_002300
Locus ID	3976
UniProt ID	P15018
Cytogenetics	22q12.2
Synonyms	CDF; DIA; HILDA; MLPLI
Summary	The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
Protein Families	Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway

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