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Isocitrate dehydrogenase (IDH1) (NM_005896) Human Over-expression Lysate
CAT＃: LC401782
IDH1 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "Isocitrate dehydrogenase" proteins (19)
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CNY 900.00
| Cited in 3 publications. |
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Specifications
| Product Data | |
| Description | IDH1 HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC210582 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 46.5 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_005887 |
| Locus ID | 3417 |
| Cytogenetics | 2q34 |
| Protein Families | WB |
| Protein Pathways | Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways |
Citations (3)
| The use of this Proteins has been cited in the following citations: |
|---|
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Antibody validation for Western blot: By the user, for the user
,null,
The Journal of Biological Chemistry
,PubMed ID 31819006
[IDH1]
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|
On the Utility of Short Echo Time (TE) Single Voxel 1H–MRS in Non–Invasive Detection of 2–Hydroxyglutarate (2HG); Challenges and Potential Improvement Illustrated with Animal Models Using MRUI and LCModel
,null,
PLoS ONE
,PubMed ID 26820720
[IDH1]
|
|
Metabolomic comparison between cells over-expressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism.
,null,
Journal of neurochemistry
,PubMed ID 25251602
[IDH1]
|
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| TP710049 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132C) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells |
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| TP710050 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132L) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells |
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| TP710051 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132S) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells |
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| TP710052 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132G) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells |
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