Isocitrate dehydrogenase (IDH1) (NM_005896) Human Recombinant Protein

Name: Isocitrate dehydrogenase (IDH1) (NM_005896) Human Recombinant Protein
SKU: TP710048
Price: 4020.00

CAT＃: TP710048

Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132H) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells.

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CNY 4,020.00

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TA500610

Anti-IDH1 (Isocitrate Dehydrogenase 1) mouse monoclonal antibody, clone OTI2H9 (formerly 2H9)

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Specifications

Product Data
Species	Human
Expression Host	Sf9
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Two DNA sequences from TrueORF clone, RC210582 and RC400096, respectively encoding human full-length IDH1 and Mutant IDH1(R132H)
Tag	C-DDK
Predicted MW	47 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity	Enzymatic activities were determined by monitoring NADPH formation based on the absorbance at 345nm. The reaction was carried out at 37? for 10 minutes in the presence of isocitrate as a substrate and NADP as a cofactor. The data which presented a good linear relation on the curve was used to calculate the specific activity, and one unit is defined as converting 1.0 umole of NADP to NADPH per min at 37?. In summary, the wildtype IDH1 produced from HEK293 cells and insect cells are active while the R132H mutant or the WT/R132H heterodimers are inactive.
Reference Data
RefSeq	NP_005887
Locus ID	3417
UniProt ID	O75874
Refseq Size	2339
Cytogenetics	2q34
Refseq ORF	1245
Synonyms	HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD
Summary	Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
Protein Pathways	Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways

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Other Versions

SKU	Description	Size	Price
LC401782	IDH1 HEK293T cell transient overexpression lysate (as WB positive control)	20 ug 产品选项验证失败所有选项都是必需的	CNY 900.00
LY401782	Transient overexpression lysate of isocitrate dehydrogenase 1 (NADP+), soluble (IDH1)	100 ug 产品选项验证失败所有选项都是必需的	CNY 3,080.00
PH310582	IDH1 MS Standard C13 and N15-labeled recombinant protein (NP_005887)	10 ug 产品选项验证失败所有选项都是必需的	CNY 19,520.00
TP310582	Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), 20 µg	20 ug 产品选项验证失败所有选项都是必需的	CNY 2,900.00 CNY 6,650.00
TP700038	Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132H),with C-terminal Myc-DDK tag, expressed in HEK293 cells	20 ug 产品选项验证失败所有选项都是必需的	CNY 6,650.00
TP700039	Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132C),with C-terminal Myc-DDK tag, expressed in HEK293 cells	20 ug 产品选项验证失败所有选项都是必需的	CNY 6,650.00
TP700040	Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132L),with C-terminal Myc-DDK tag, expressed in HEK293 cells	20 ug 产品选项验证失败所有选项都是必需的	CNY 6,650.00
TP700041	Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132S),with C-terminal Myc-DDK tag, expressed in HEK293 cells	20 ug 产品选项验证失败所有选项都是必需的	CNY 6,650.00
TP700042	Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132G),with C-terminal Myc-DDK tag, expressed in HEK293 cells	20 ug 产品选项验证失败所有选项都是必需的	CNY 6,650.00
TP710042	Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), with C-terminal polyhistidine tag, expressed in sf9 cells.	20 ug 产品选项验证失败所有选项都是必需的	CNY 4,020.00
TP710043	Recombinant protein of mutant(R132H) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells.	20 ug 产品选项验证失败所有选项都是必需的	CNY 4,020.00
TP710044	Recombinant protein of mutant(R132C) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells	20 ug 产品选项验证失败所有选项都是必需的	CNY 4,020.00
TP710045	Recombinant protein of mutant(R132L) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells.	20 ug 产品选项验证失败所有选项都是必需的	CNY 4,020.00
TP710046	Recombinant protein of mutant(R132S) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells.	20 ug 产品选项验证失败所有选项都是必需的	CNY 4,020.00
TP710047	Recombinant protein of mutant(R132G) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells.	20 ug 产品选项验证失败所有选项都是必需的	CNY 4,020.00
TP710049	Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132C) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells	20 ug 产品选项验证失败所有选项都是必需的	CNY 4,020.00
TP710050	Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132L) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells	20 ug 产品选项验证失败所有选项都是必需的	CNY 4,020.00
TP710051	Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132S) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells	20 ug 产品选项验证失败所有选项都是必需的	CNY 4,020.00
TP710052	Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132G) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells	20 ug 产品选项验证失败所有选项都是必需的	CNY 4,020.00

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