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GAA (NM_000152) Human Over-expression Lysate
CAT＃: LC400052
GAA HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "GAA" proteins (12)
|
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CNY 900.00
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CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
| Product Data | |
| Description | GAA HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC215796 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 105.32 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_000143 |
| Locus ID | 2548 |
| Cytogenetics | 17q25.3 |
| Protein Families | WB |
| Protein Pathways | Galactose metabolism, Lysosome, Metabolic pathways, Starch and sucrose metabolism |
Documents
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC421540 | GAA HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 1,470.00 |
|
| LC421541 | GAA HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY400052 | Transient overexpression lysate of glucosidase, alpha; acid (GAA), transcript variant 1 |
CNY 3,080.00 |
|
| LY421540 | Transient overexpression lysate of glucosidase, alpha; acid (GAA), transcript variant 2 |
CNY 4,840.00 |
|
| LY421541 | Transient overexpression lysate of glucosidase, alpha; acid (GAA), transcript variant 3 |
CNY 3,080.00 |
|
| PH308033 | GAA MS Standard C13 and N15-labeled recombinant protein (NP_001073272) |
CNY 19,520.00 |
|
| PH315796 | GAA MS Standard C13 and N15-labeled recombinant protein (NP_000143) |
CNY 19,520.00 |
|
| PH315840 | GAA MS Standard C13 and N15-labeled recombinant protein (NP_001073271) |
CNY 19,520.00 |
|
| TP308033 | Recombinant protein of human glucosidase, alpha; acid (GAA), transcript variant 3, 20 µg |
CNY 2,900.00 |
|
| TP315796 | Recombinant protein of human glucosidase, alpha; acid (GAA), transcript variant 1, 20 µg |
CNY 2,900.00 |
|
| TP315840 | Recombinant protein of human glucosidase, alpha; acid (GAA), transcript variant 2, 20 µg |
CNY 2,900.00 |
|
| TP701073 | Purified recombinant protein of Human glucosidase, alpha, acid (GAA), with N-terminal His tag, secretory expressed in HEK293 cells, 20ug |
CNY 7,106.00 |
