REV1 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	REV1
Locus ID	51455
Kit Components	KN217528G1, REV1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AAATGATGGCTGGGAAACAT KN217528G2, REV1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGGAAGCGAGCTGAAAATGA KN217528-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGGG TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG TTGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTACTATGGT TGCTTTGACG TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGGAG GCTACAGTCA GTGGAGAGGA CTTTCACAGG CTGTCGCCGT GCTCATTTGA TAACTGCCCG TTATTCATGC GACACTTTTA TAGTATAGTT AGTTAAGTAA GTGTTGCCTT AAGGAGAATG ACAGATTTAC TATTATAGTT TATTATCAAT GATAATCTTT TCTGCATCAT TTTTAATTGA CTTCTTCTAG GGCTTTGGCA GAATATGTGA ATCAGGCTAT GACTGAACTC TTAAGTGTCC AGTATGTAAT AGCAAAGACA GAGTTTAAAA GCCACACTAT AGGACAACTC ATGAAGGGAA ATGTTTTGTT AGATTGTCTT ATATAATGGT AACAAGTAAA GTAAGCATGG CCATGTTAGT TGGATTTGGT GTTTTATGTA AGTGAATGTT AGGATTGGTG CTCAGGAATG CATAAAGATA TTTTTCTATT GAATTTTAAA TATAAGAGTT ACAATTTAAA CATGTGTAGG AGGGTGTTTT ATAGACAAAT TTCACTTGTA AAGCTGGAGA ATGGAGAACT TCAGAAAAAG TTTACTTAAT ATTGTATACT TTCATCAGTT GGACATTTGA ATTTAATTTC TCATTTTCTT GATATTGAAA TAAAATATCA TTTAGAAATA CATTATGTTA TAAAATTGAC ATTTTTTTTT TCCTCCCCAA TACAGAAGCT CCACCAAAAT AATTATATCG AACTGTCTAG TTATTTTTCA TTCTTCTGTT ATAATGTGGT TTAAGTTGTT TTCTAAATTG TAAATTACTT TAGACACCAC ATCTTTGAGT TTTCACAGTG TATTGAAAGC CTTATGATGT TTATAAACTT TATCCCCTAA ACTTTACTCT CAGGAATTTT ACCCTGAGGA AATAAATATG CCTAGTGCAC AATAATTTAC TATATGATGA TTTTATTGCA GCATTGCTAA TAATACAGAA AAACAGGAAA ACACATAAAT ATCCAATAAT AAGGGATTGG TCAAATAAGT TATCTGTGTA TAGTGGAGCA CTGTACAGTC ATTATTCAGC TTTATATATC TCAACCTGGA AGGATAACCT TGACTAAAAC AATGTGTTAC AAAGAAATAT TTTGTATGAT TATATGTTTG TAAAACCTCT GTGCTCTGAG TTATGGAAAT AAGCTTTGAA AACAACACCC CAAAGTTTTA ATGAATAGGA TTATACCAGT TTTAATTTTC TTCCCCATAT TTCCTGTATT TTCTGAAAAG AAACTTCATA GTGAGCATAT AATATTTTAA TAATCAGCAA AAATAATGCA GTCAT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001037872, NM_001321454, NM_001321455, NM_001321458, NM_001321459, NM_001321460, NM_016316, NR_135649, NR_135650, NR_135651, NR_135652, NR_135653
Synonyms	AIBP80; REV1L
Summary	This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016]

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