USP28 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
Format | 2 gRNA vectors, 1 linear donor |
Donor DNA | EF1a-GFP-P2A-Puro |
USP28 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
Format | 2 gRNA vectors, 1 linear donor |
Donor DNA | EF1a-GFP-P2A-Puro |
NLRP1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
Format | 2 gRNA vectors, 1 linear donor |
Donor DNA | EF1a-GFP-P2A-Puro |
qSTAR qPCR primer pairs against Homo sapiens gene GAPDH
Oligo annealing buffer (10X)
SensiMix SYBR master mix with Hi-ROX, 50 x 25 uL rxn
SensiMix SYBR master mix with Hi-ROX, 400 x 25 uL rxn
Cre recombinase mammalian expression vector
PCR Primer mix to confirm AAVS1 integration, amplifying the left integration junction, 2 nmols
SensiMix SYBR master mix with fluorescein, 400 x 25 uL rxn
qSTAR qPCR primer pairs against Homo sapiens gene PPIA
Forward primer to sequence targets cloned in expression vectors.
SensiMix SYBR master mix with Low-ROX, 400 x 25 uL rxn
EIF2AK3 - human gene knockout kit via CRISPR, HDR mediated
Format | 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control |
Donor DNA | GFP-puro |
CF3, the forward sequencing primer to sequence target sequence cloned in CRISPR vectors.
Cas9 in vitro digestion buffer, 10x