NLRP1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
Format | 2 gRNA vectors, 1 linear donor |
Donor DNA | EF1a-GFP-P2A-Puro |
NLRP1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
Format | 2 gRNA vectors, 1 linear donor |
Donor DNA | EF1a-GFP-P2A-Puro |
qSTAR qPCR primer pairs against Homo sapiens gene GAPDH
Oligo annealing buffer (10X)
SensiMix SYBR master mix with Hi-ROX, 50 x 25 uL rxn
SensiMix SYBR master mix with Hi-ROX, 400 x 25 uL rxn
Cre recombinase mammalian expression vector
PCR Primer mix to confirm AAVS1 integration, amplifying the left integration junction, 2 nmols
SensiMix SYBR master mix with fluorescein, 400 x 25 uL rxn
qSTAR qPCR primer pairs against Homo sapiens gene PPIA
Forward primer to sequence targets cloned in expression vectors.
SensiMix SYBR master mix with Low-ROX, 400 x 25 uL rxn
CF3, the forward sequencing primer to sequence target sequence cloned in CRISPR vectors.
Cas9 in vitro digestion buffer, 10x
qSTAR qPCR primer pairs against Homo sapiens gene DSPP
Reverse primer to sequence targets cloned in expression vectors.