LMX1A Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	LMX1A
Locus ID	4009
Kit Components	KN215375G1, LMX1A gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCAAAGCGCGATCGACACCT KN215375G2, LMX1A gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGTGTTCAGGCCGTGCGTCC KN215375-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CGCACCCCAA CAGAGCGCAG CGCCAGGAGT CCAGAAGCGG GCGGGACGCC CTCCGGGTCC CTTACAGTGC CCCTTCTCGA CCTGGGGCAG GTGAGGGCCG CAACGGGGCG GCTGGGACGC GGGATTGCAA ACCCCATCGT CCCGCGTGCC TGGACCCGGT CGCTCGAGGG AGGGTACCCA CTCTTTATAT ACCCAATATA CCCCAGTAGC TGCGTTCCTG CAGAGACGTC CCGAGGGCCC ACCTTCGTAT AGGTTGGGGC GGAGTCGGAT TCGGGATGGA AAACCTGGGG CAAGGGATGT AGGTGGGGGT GAGGGGGGCA GGAGAAGGAG AAACGCAGTT GGGGGGCGGA GGCCTAAGTA CATAACGTGT TGACTTCAAG TGAAATCAGA TCAGCCAGAG CAGTTCGCTG TGACTGATCT CTCCTCCCAC CCTACATTCT CTTGGCTGGA CCCTATCCTC CTGGCTGATT CTGGTCGCCC TGGACACTCC CTCAGTTCTT TCCCAGGAGT GCGGTGGCTG CTGGCGCCGA GTCCCAGCGG GCACGGACGT CAGACGCATC GTTTCTTCTC CTCTACAGGT CCTCCCGGCC CGGCCCGAAC TGGGCGCACT CTCTTTCCGC TTGGCGCTGA GCTCTGGAGC CCCGCTCTCT GGGACCTGGT CCGCGATAGG GAAGCTAGCG CCCCTCTTCA TACACTAAAT TGAGCCCCAT CACTATCTGT CCGTCAGTGC TTGTGGGTCG TCCCTACCCA AATAAATCCA ACAAGCCGCC CCAGGCCTCA CGCACTGGGC ACCGAATTCC CCAAAGCCGC GAGGGGCGGG CGAGCTTGTT CGTAGGCGTC TGAGTGGCAA GTGATTAAAA ATACCCAGGG CTGGATTTTT AATCTCGGAG CTGATCGACG TCTCATAAAT GCCGCCCTCT TCTCGCGGCC TAGAGGCAAT AGCATCCGAG ACCCGAGGCC TGGAGCGCCC AAGTTCGAGG AGGCTTCTCT CCCCCACCAA CTCCAGCCCC AATTTCAGCC ATGGGCAAGG CCGAGAGAGA CTTTTCTGGG CCAGTAGGCA ACGCAGCGCG GGGATTAGAC CGCGCGGCTG GGCCCTAGGC TCCGCGTTAA TTAACTGGAG CTGGATGTCG GGTCCTGTGG GTCCCCCCTC ACAACTCCTT TGCAGCGACA GAGGAGGAGC AGCGAGTCAA GGACCCCGGA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001033507, NM_001174069, NM_177398, NM_177399
Synonyms	dr; dreher; LIM homeobox transcription factor 1 alpha; LMX-1; LMX1; LMX1.1; Lmx1.1; MGC87616; MGC129356; MGC129357; OTTMUSP00000022889; OTTMUSP00000022890; shaker short-tail; sst
Summary	This gene encodes a homeodomain and LIM-domain containing protein. The encoded protein is a transcription factor that acts as a positive regulator of insulin gene transcription. This gene also plays a role in the development of dopamine producing neurons during embryogenesis. Mutations in this gene are associated with an increased risk of developing Parkinson's disease. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

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