HIF 2 alpha (EPAS1) Human Gene Knockout Kit (CRISPR)

CAT#: KN208604LP

EPAS1 - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

  See Other Versions

CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


EPAS1 mouse monoclonal antibody, clone OTI2G5 (formerly 2G5)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


EPAS1 (Myc-DDK-tagged)-Human endothelial PAS domain protein 1 (EPAS1)
    • 10 ug

CNY 9,560.00

Specifications

Product Data
Format 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA Luciferase-Puro
Symbol HIF 2 alpha
Locus ID 2034
Kit Components

KN208604G1, HIF 2 alpha gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCTCGGACCGGCCCCCTGAT

KN208604G2, HIF 2 alpha gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGCGGGCGTCCGGGCCGATC

KN208604LP-D, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet

GCCGGTGCCC GCCCCCCGCC CTCCGCGCCC GCCCCGTGCC CGCCCCAAGC CGGCCGACGG AGTTTTTAAA GTGGGCTGCC GGCCGCGGGA GCTTTACACT CGCGAGCGGA CCGCCACACG GGTCCGGTGC CCGCTGCGCT TCCGCCCCAG CGCTCCTGAG GCGGCCGTAC AATCCTCGGC AGTGTCCTGA GACTGTATGG TCAGCTCAGC CCGGCCTCCG ACTCCTTCCG ACTCCCAGCA TTCGAGCCAC TTTTTTTTTT CTTTGAAAAC TCAGAAAAGT GACTCCTTTT CCAGGGAAAA AGGAACTTGG GTTCCCTTCT CTCCGTCCTC TTTTCGGGTC TGACAGCCTC CACCCACTCC TTCCCCGGAC CCCGCCTCCG CGCGCAGGTT CCTCCCAGTC ACCTTTCTCC ACCCCCGCCC CCGCACCTAG CCCGCCGCGC GCCACCTTCC ACCTGACTGC GCGGGGCGCT CGGGACCTGC GCGCACCTCG GACCTTCACC ACCCGCCCGG GCCGCGGGGA GCGGACGAGG GCCACAGCCC CCCACCCGCC AGGGAGCCCA GGTGCTCGGC GTCTGAACGT CTCAAAGGGC CACAGCGACA AGGCGCGACC GAGAGTGGTT GGGAGAAGAG TGCTGAGAGG TCTTCGGAGC TCGAGGCTCG GTTTGGAGGG CTGTGAGGGG GAGAGCATGT GCCCGGTTTG GGGGCGCGGA TCAGCAGCTT TGCAGTGGAG ACTTCTGCGG CTCGGAGGAG TCGGGGATTT GCGCGCACGG CGAGGCCAGG AGGCCGAGGG AGATGGCCTG GAGGTCGGAG GTGCTTCCGC GGTGCCTTTG AAAATCTCCC AGCCGCCCCG CAGCAGATTA CCGTGGCCAC CGGCGCTTGG AAATGTTTGT GGGTGCATGT CCTCGACTTT CTCTGGTCTC TCATTTTGGG GCAGCATCCA CGTCTCTATT TTTCTCTGGA TTCGCGGAGT CCTTCCAAAT GCGCTCCTGT GCCCGCGCCG CGGCCCAAGT GGGCAAAGGG GGGCGGGAGG CGGAGAGCGG CTCAGGGACA CGATCCTACC GAGGAGCCAG GACCCTTCAG AGCGCCCCGC TGCCGGGCTC CAGACCCCAC CGAAGGTTGG AGAACCCACT TCCTCGTGCC GCACCTTGAC TCTGGGGAGA GTAGGTAGTG AGTGACCTGG ATTGCCCTGC GGGGGCAGAT

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001430
Synonyms bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2
Summary This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Other Versions

Customer Reviews 
Loading...