HIF 2 alpha (EPAS1) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	HIF 2 alpha
Locus ID	2034
Kit Components	KN208604G1, HIF 2 alpha gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCTCGGACCGGCCCCCTGAT KN208604G2, HIF 2 alpha gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGCGGGCGTCCGGGCCGATC KN208604-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GCCGGTGCCC GCCCCCCGCC CTCCGCGCCC GCCCCGTGCC CGCCCCAAGC CGGCCGACGG AGTTTTTAAA GTGGGCTGCC GGCCGCGGGA GCTTTACACT CGCGAGCGGA CCGCCACACG GGTCCGGTGC CCGCTGCGCT TCCGCCCCAG CGCTCCTGAG GCGGCCGTAC AATCCTCGGC AGTGTCCTGA GACTGTATGG TCAGCTCAGC CCGGCCTCCG ACTCCTTCCG ACTCCCAGCA TTCGAGCCAC TTTTTTTTTT CTTTGAAAAC TCAGAAAAGT GACTCCTTTT CCAGGGAAAA AGGAACTTGG GTTCCCTTCT CTCCGTCCTC TTTTCGGGTC TGACAGCCTC CACCCACTCC TTCCCCGGAC CCCGCCTCCG CGCGCAGGTT CCTCCCAGTC ACCTTTCTCC ACCCCCGCCC CCGCACCTAG CCCGCCGCGC GCCACCTTCC ACCTGACTGC GCGGGGCGCT CGGGACCTGC GCGCACCTCG GACCTTCACC ACCCGCCCGG GCCGCGGGGA GCGGACGAGG GCCACAGCCC CCCACCCGCC AGGGAGCCCA GGTGCTCGGC GTCTGAACGT CTCAAAGGGC CACAGCGACA AGGCGCGACC GAGAGTGGTT GGGAGAAGAG TGCTGAGAGG TCTTCGGAGC TCGAGGCTCG GTTTGGAGGG CTGTGAGGGG GAGAGCATGT GCCCGGTTTG GGGGCGCGGA TCAGCAGCTT TGCAGTGGAG ACTTCTGCGG CTCGGAGGAG TCGGGGATTT GCGCGCACGG CGAGGCCAGG AGGCCGAGGG AGATGGCCTG GAGGTCGGAG GTGCTTCCGC GGTGCCTTTG AAAATCTCCC AGCCGCCCCG CAGCAGATTA CCGTGGCCAC CGGCGCTTGG AAATGTTTGT GGGTGCATGT CCTCGACTTT CTCTGGTCTC TCATTTTGGG GCAGCATCCA CGTCTCTATT TTTCTCTGGA TTCGCGGAGT CCTTCCAAAT GCGCTCCTGT GCCCGCGCCG CGGCCCAAGT GGGCAAAGGG GGGCGGGAGG CGGAGAGCGG CTCAGGGACA CGATCCTACC GAGGAGCCAG GACCCTTCAG AGCGCCCCGC TGCCGGGCTC CAGACCCCAC CGAAGGTTGG AGAACCCACT TCCTCGTGCC GCACCTTGAC TCTGGGGAGA GTAGGTAGTG AGTGACCTGG ATTGCCCTGC GGGGGCAGAT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001430
Synonyms	bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2
Summary	This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]

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