Interleukin 34 (IL34) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Interleukin 34
Locus ID	146433
Kit Components	KN206629G1, Interleukin 34 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCTATGGTTACGGCAGATCC KN206629G2, Interleukin 34 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CCTGGCTGCGCTGTGAGTAC KN206629-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CTGCCAGCTG GTGTCATCAT AACTCACTCA TGGGCCAGGG CCGTTCGGAC ATCCTGAAGT CATTTCTCAC GTGAGCCGTG TCCAGCTGCC AGGGCCGGGT GGTCCTGTGC AGGCCGTGGG TGCGGTGTGC GCTGTGGCTG ATGGCTGAGT GGCAGCGCTG CTCTTACATA ATCAGTTTCT CTCCTCCTTT CTCTTCCTAG AGCCAGATTT CACACTGAGC AGCTGCAGTC GGAGAAATCA GAGAAAGCGT CACCCAGCCC CAGATTCCGA GGGGCCTGCC AGGGACTCTC TCCTCCTGCT CCTTGGAAAG GAAGACCCCG AAAGACCCCC AAGCCACCGG CTCAGACCTG CTTCTGGGCT GCCATGGGAC TTGCGGCCAC CGCCCCCCGG CTGTCCTCCA CGCTGCCGGG CAGATAAGGG CAGCTGCTGC CCTTGGGGCA CCTGCTCACT CCCGCAGCCC AGCCACTCCT CCAGGGCCAG CCCTTCCCTG ACTGAGTGAC CACCTCTGCT GCCCCGAGGC CATGTAGGCC GTGCTTAGGC CTCTGTGGAC ACACTGCTGG GGACGGCGCC TGAGCTCTCA GGGGGACGAG GAACACCACC AACCAGGGCA TTCTTGCTGG TGAGAAGTTG CGATGCTTCC CAGCCGGACT GCAGACACCC TCTGAGATTC CCACGGCCGC CGTCTGCCCT GTCTGCCGCT CACTTCGGGC TCCGACGGGC AAGCTGGGTG GGCAGTTCTG GCTGGGTGGG CATGGTCCTC AGGGTGGTCT TAGGTCGGGG CAGGCACTGG GGGTAGGTGG CAGCATCTCA CCTTTCCAGG CTTGTGGGTG TTGGGGAGCC CCGGGCGGGC AGCAGCGGCC CAGTCCTGGG TATGGCCGGT GGAGGGGGGC AGGGGCTGGT GAAAGTGTCT AGGGACCCAA GACCCACGGC AGAAGAGCAG CCAGATGCCT GGAGAAGGCA GCCTGTTCCC CTCCAACCCT CCCTCCATAA CAGAACAGAA TTAAAAAGCA GAAAGATAAC ACTGTTGGTA AAAAGAAAAA TAAGTTGAAA TGAGGACAGA AAGGAAAAAA AGGATCTGGA AGCCTCTTAT GCCTGCTGGC TGGGAGGCCA GACCAAGATC CCCTCAGTCT CCAAGATCCA GTTCAATTGA CTCAAATTCA ACACATTTTT CCGGTGCCTC CCACGTGCCC ACTGCAGAGT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001172771, NM_001172772, NM_152456
Synonyms	C16orf77; IL-34
Summary	Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.