Interleukin 34 (IL34) (NM_152456) Human Tagged ORF Clone
CAT#: RC206629
IL34 (Myc-DDK-tagged)-Human interleukin 34 (IL34), transcript variant 1
ORF Plasmid: tGFP
"NM_152456" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Cited in 2 publications. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C16orf77; IL-34 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206629 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGGGGCTTCACCTGGCTGCGCTATCTTGGGATCTTCCTTGGCGTGGCCTTGGGGAATGAGCCTT TGGAGATGTGGCCCTTGACGCAGAATGAGGAGTGCACTGTCACGGGTTTTCTGCGGGACAAGCTGCAGTA CAGGAGCCGACTTCAGTACATGAAACACTACTTCCCCATCAACTACAAGATCAGTGTGCCTTACGAGGGG GTGTTCAGAATCGCCAACGTCACCAGGCTGCAGAGGGCCCAGGTGAGCGAGCGGGAGCTGCGGTATCTGT GGGTCTTGGTGAGCCTCAGTGCCACTGAGTCGGTGCAGGACGTGCTGCTCGAGGGCCACCCATCCTGGAA GTACCTGCAGGAGGTGCAGACGCTGCTGCTGAATGTCCAGCAGGGCCTCACGGATGTGGAGGTCAGCCCC AAGGTGGAATCCGTGTTGTCCCTCTTGAATGCCCCAGGGCCAAACCTGAAGCTGGTGCGGCCCAAAGCCC TGCTGGACAACTGCTTCCGGGTCATGGAGCTGCTGTACTGCTCCTGCTGTAAACAAAGCTCCGTCCTAAA CTGGCAGGACTGTGAGGTGCCAAGTCCTCAGTCTTGCAGCCCAGAGCCCTCATTGCAGTATGCGGCCACC CAGCTGTACCCTCCGCCCCCGTGGTCCCCCAGCTCCCCGCCTCACTCCACGGGCTCGGTGAGGCCGGTCA GGGCACAGGGCGAGGGCCTCTTGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206629 protein sequence
Red=Cloning site Green=Tags(s) MPRGFTWLRYLGIFLGVALGNEPLEMWPLTQNEECTVTGFLRDKLQYRSRLQYMKHYFPINYKISVPYEG VFRIANVTRLQRAQVSERELRYLWVLVSLSATESVQDVLLEGHPSWKYLQEVQTLLLNVQQGLTDVEVSP KVESVLSLLNAPGPNLKLVRPKALLDNCFRVMELLYCSCCKQSSVLNWQDCEVPSPQSCSPEPSLQYAAT QLYPPPPWSPSSPPHSTGSVRPVRAQGEGLLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_152456 |
ORF Size | 726 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_152456.1, NP_689669.1 |
RefSeq Size | 1796 bp |
RefSeq ORF | 729 bp |
Locus ID | 146433 |
UniProt ID | Q6ZMJ4 |
MW | 27.5 kDa |
Gene Summary | Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
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Human Interleukin-34 facilitates microglia-like cell differentiation and persistent HIV-1 infection in humanized mice
,Mathews, S;Branch Woods, A;Katano, I;Makarov, E;Thomas, MB;Gendelman, HE;Poluektova, LY;Ito, M;Gorantla, S;,
Mol Neurodegener
,PubMed ID 30832693
[Interleukin 34]
|
IKKβ and mutant huntingtin interactions regulate the expression of IL-34; implications for microglial-mediated neurodegeneration in HD
,Khoshnan, A;Sabbaugh, A;Calamini, B;Marinero, SA;Dunn, DE;Yoo, JH;Ko, J;Lo, DC;Patterson, PH;,
Human Molecular Genetics
,PubMed ID 28973132
[Interleukin 34]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206629L1 | Lenti ORF clone of Human interleukin 34 (IL34), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC206629L2 | Lenti ORF clone of Human interleukin 34 (IL34), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC206629L3 | Lenti ORF clone of Human interleukin 34 (IL34), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC206629L4 | Lenti ORF clone of Human interleukin 34 (IL34), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG206629 | IL34 (tGFP-tagged) - Human interleukin 34 (IL34), transcript variant 1 |
CNY 4,000.00 |
|
SC100981 | IL34 (untagged)-Human interleukin 34 (IL34), transcript variant 1 |
CNY 2,400.00 |
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