HIF-1 alpha (HIF1A) Human Gene Knockout Kit (CRISPR)

CAT#: KN202461LP

HIF1A - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

  See Other Versions

CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


Rabbit Polyclonal HIF-1 alpha Antibody
    • 100 ul

CNY 6,056.00


HIF1A (Myc-DDK-tagged)-Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1
    • 10 ug

CNY 6,048.00

Specifications

Product Data
Format 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA Luciferase-Puro
Symbol HIF-1 alpha
Locus ID 3091
Kit Components

KN202461G1, HIF-1 alpha gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TTTCTTGTCGTTCGCGCCGC

KN202461G2, HIF-1 alpha gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGGCGGCGGGCCGAGGGAAT

KN202461LP-D, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet

ATCTCCTTTC TCTTTCCTCC GCCGCTAAAC ACAGACGAGC ACGTGAGCGT CGCAGCCCGT CCCAGCTGTG CCTCAGCTGA CCGCCTCCTG ATTGGCTGAG AGCGGCGTGG GCTGGGGTGG GGACTTGCCG CCTGCGTCGC TCGCCATTGG ATCTCGAGGA ACCCGCCTCC ACCTCAGGTG AGGCGGGCTT GCGGGAGCGC GCGCCGGCCT GGGCAGGCGA GCGGGCGCGC TCCCGCCCCC TCTCCCCTCC CCGCGCGCCC GAGCGCGCCT CCGCCCTTGC CCGCCCCCTG ACGCTGCCTC AGCTCCTCAG TGCACAGTGC TGCCTCGTCT GAGGGGACAG GAGGATCACC CTCTTCGTCG CTTCGGCCAG TGTGTCGGGC TGGGCCCTGA CAAGCCACCT GAGGAGAGGC TCGGAGCCGG GCCCGGACCC CGGCGATTGC CGCCCGCTTC TCTCTAGTCT CACGAGGGGT TTCCCGCCTC GCACCCCCAC CTCTGGACTT GCCTTTCCTT CTCTTCTCCG CGTGTGGAGG GAGCCAGCGC TTAGGCCGGA GCGAGCCTGG GGGCCGCCCG CCGTGAAGAC ATCGCGGGGA CCGATTCACC TCGGCGTTAA TGGGATTGGG GGGGGCAGCC TTTTTGTTTC TGCTGCTGCT CCCCTCCCCT CTCTTCCCCC AACCTCGCCG GCCGGGCTCC CCCGCTGTCC ACGTCGCCAT CTTGTCGTGG GGGGTGGGAG ACGCCTCGAA AGTGCTTTCA GGGGCCGGGG TCTGAGCCCT GCTTGCCCTC CCCGCCGGCC GTGGGGGCCT CGCGCCGCCC ACCTACCCGC CTCAAAAACC CAGCCTGCTC TGTGGCCCCA TCCGGAGGGG ACTTTACCCA GCCTGAAAAC CCCGGGAAGA GAAATGAGCT GCAGCTCGGT AGCCGCGGTT TGCACCCGGA GCTTCCGCTC CTTCCCGCCC CCATCCTCTC CAGTTCCATT GAAAACTCGG CCCTGGGGCG GACCCTGCAC GCTGGTCCTG GCTTTCCAGT GGACTTGGGG CCTTGAGTTC CCGACTGAGG GACTCGCGTG GTCGGATGCG ATCTTGTCCT GTAGTTGTCC AGCCGTCGCG GGTGTCTTTG CCTTTGTGCA TTAGGGATTT GCCGCGATGG CCTTAAGATG CGAACTTTTT AGTTTGCACG TGCAGGTTTT GTTTCGTTTT AATCGCCTTG

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001243084, NM_001530, NM_181054
Synonyms bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
Summary This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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