GBA Human Gene Knockout Kit (CRISPR)

CAT#: KN201614

GBA - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

  See Other Versions

CNY 12,260.00


货期*
4周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


GBA mouse monoclonal antibody, clone OTI4G4 (formerly 4G4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


GBA (Myc-DDK-tagged)-Human glucosidase, beta, acid (GBA), transcript variant 1
    • 10 ug

CNY 3,992.00

Specifications

Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA GFP-puro
Symbol GBA
Locus ID 2629
Kit Components

KN201614G1, GBA gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CAGCATTGTCACAGTGCTTC

KN201614G2, GBA gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GTTTTCAAGTCCTTCCAGAG

KN201614-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

TAGGGCAAAG ACTGTCTTAA ACATCTTGGT AGTGTCAGTA TTTTGCACAG TGAAGTTTTT TTTTTTAAAT TATATCAGCT TTATTTGTAC CTTTTTGACA TTTCTATCAA AAAAGAAGTG TGCCTGCTGT GGTTCCCATC CTCTGGGATT TAGGAGCCTC TACCCCATTC TCCATGCAAA TCTGTGTTCT AGGCTCTTCC TAAAGTTGTC ACCCATACAT GCCCTCCAGA GTTTTATAGG GCATATAATC TGTAACAGAT GAGAGGAAGC CAATTGCCCT TTAGAAATAT GGCTGTGATT GCCTCACTTC CTGTGTCATG TGACGCTCCT AGTCATCACA TGACCCATCC ACATCGGGAA GCCGGAATTA CTTGCAGGGC TAACCTAGTG CCTATAGCTA AGGCAGGTAC CTGCATCCTT GTTTTTGTTT AGTGGATCCT CTATCCTTCA GAGACTCTGG AACCCCTGTG GTCTTCTCTT CATCTAATGA CCCTGAGGGG TGGAATCCTG GCACTGGAAT TTAATGAATG ACAGACTCTC TTTGAATCCA GGGCCATCAT GGCTCTTTGA GCAAGGCACA GATGGAGGGA GGGGTCGAAG TTGAAATGGG TGGGAAGAGT GGTGGGGAGC ATCCTGATTT GGGGTGGGCA GAGAGTTGTC ATCAGAAGGG TTGCAGGGAG AGCTGCACCC AGGTTTCTGT GGGCCTTGTC CTAATGAATG TGGGAGACCG GGCCATGGGC ACCCAAAGGC AGCTAAGCCC TGCCCAGGAG AGTAGTTGAG GGGTGGAGAG GGGCTTGCTT TTCAGTCATT CCTCATTCTG TCCTCAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGAG TGAGTCAAGG CAGTGGGGAG GTAGCACAGA GCCTCCCTTC TGCCTCATAG TCCTTTGGTA GCCTTCCAGT AAGCTGGTGG

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_000157, NM_001005741, NM_001005742, NM_001005749, NM_001005750, NM_001171811, NM_001171812
Synonyms GBA1; GCB; GLUC
Summary This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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