SARS-CoV-2 E Gene Tagged ORF (codon optimized)

CAT＃: VC102565

TrueORF^®

Myc-DDK-tagged ORF clone for SARS-CoV-2 Envelop Protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724392. Note: ORF is codon optimized

Datasheet

SDS

Plasmid Map

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CNY 1,200.00

CNY 3,800.00

货期*

现货

规格

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Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >VC102565 representing NC_045512, codon optimized. Due to the complexity of NC_045512, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTATTCCTTCGTGTCTGAAGAAACTGGTACCCTCATTGTCAATTCTGTCTTGCTGTTCCTCGCCTTC GTCGTCTTCCTCCTTGTCACCCTTGCCATTCTTACTGCTCTCCGCCTCTGTGCCTATTGTTGTAATATC GTTAATGTCTCCCTGGTTAAACCTTCTTTCTATGTCTACTCCCGCGTAAAAAACCTCAACTCCTCACGG GTTCCCGATCTCCTTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >VC102565 representing NC_045512 Blue=ORF Red=Cloning site Green=Tag(s) MYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCCNIVNVSLVKPSFYVYSRVKNLNSSR VPDLLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NC_045512
ORF Size	225 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	YP_009724392
RefSeq ORF	225 bp
MW	8.4 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC102555	Myc-DDK-tagged ORF clone for SARS-CoV-2 nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724397	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 3,800.00
VC102556	Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein extracellular domain [Severe acute respiratory syndrome coronavirus 2], YP_009724390	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,320.00
VC102557	Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724390. Note: ORF is codon optimized	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,864.00
VC102558	Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724391. Note: ORF is codon optimized	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC102559	Myc-DDK-tagged ORF clone for SARS-CoV-2 Membrane Glycoprotein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724393. Note: ORF is codon optimized	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC102560	Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724394	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102561	Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724395	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102562	Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724396	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102563	Myc-DDK-tagged ORF clone for SARS-CoV-2 Nucleocapsid Phosphoprotein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724397	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 3,800.00
VC102564	Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009725255	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102566	Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724390	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,864.00
VC102567	Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], YP_009724391	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC102568	Myc-DDK-tagged ORF clone for SARS-CoV-2 Membrane Glycoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724393	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC102569	Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], YP_009724394	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102570	Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], YP_009724395	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102571	Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], YP_009724396	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102572	Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], YP_009725255	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102573	Myc-DDK-tagged ORF clone for SARS-CoV-2 Envelop Protein [Severe acute respiratory syndrome coronavirus 2], YP_009724392	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102574	mRFP-tagged ORF clone for SARS-CoV-2 NSP4 [Severe acute respiratory syndrome coronavirus 2], YP_009725300.1	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,840.00
VC102575	mRFP-tagged ORF clone for SARS-CoV-2 NSP5 [Severe acute respiratory syndrome coronavirus 2], YP_009725301.1	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC102576	mRFP-tagged ORF clone for SARS-CoV-2 NSP6 [Severe acute respiratory syndrome coronavirus 2], YP_009725302.1	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC102577	mRFP-tagged ORF clone for SARS-CoV-2 NSP7 [Severe acute respiratory syndrome coronavirus 2], YP_009725303.1	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102578	mRFP-tagged ORF clone for SARS-CoV-2 NSP8 [Severe acute respiratory syndrome coronavirus 2], YP_009725304.1	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC102579	mRFP-tagged ORF clone for SARS-CoV-2 NSP9 [Severe acute respiratory syndrome coronavirus 2], YP_009725305.1	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102580	mRFP-tagged ORF clone for SARS-CoV-2 NSP10 [Severe acute respiratory syndrome coronavirus 2], YP_009725306.1	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102581	mRFP-tagged ORF clone for SARS-CoV-2 NSP14 [Severe acute respiratory syndrome coronavirus 2], YP_009725309.1	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,040.00
VC102582	mRFP-tagged ORF clone for SARS-CoV-2 ORF3a [Severe acute respiratory syndrome coronavirus 2], YP_009724391.1	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC102583	mRFP-tagged ORF clone for SARS-CoV-2 M protein [Severe acute respiratory syndrome coronavirus 2], YP_009724393.1	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC102584	mRFP-tagged ORF clone for SARS-CoV-2 ORF9b [Severe acute respiratory syndrome coronavirus 2], YP_009724397.2	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102585	mRFP-tagged ORF clone for SARS-CoV-2 ORF9c [Severe acute respiratory syndrome coronavirus 2], YP_009724397.2	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102586	mRFP-tagged ORF clone for SARS-CoV-2 ORF10 [Severe acute respiratory syndrome coronavirus 2], YP_009725255.1	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102587	Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein mutant (D614G) [Severe acute respiratory syndrome coronavirus 2], YP_009724390	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,864.00
VC202557	Native cDNA clone for SARS-CoV-2 surface glycoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724390	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,872.00
VC202558	Native cDNA clone for SARS-CoV-2 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], YP_009724391	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC202559	Native cDNA clone for SARS-CoV-2 Membrane Glycoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724393	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC202560	Native cDNA clone for SARS-CoV-2 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], YP_009724394	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC202561	Native cDNA clone for SARS-CoV-2 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], YP_009724395	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC202562	Native cDNA clone for SARS-CoV-2 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], YP_009724396	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC202563	Native cDNA clone for SARS-CoV-2 Nucleocapsid Phosphoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724397	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 3,800.00
VC202564	Native cDNA clone for SARS-CoV-2 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], YP_009725255	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC202565	Native cDNA clone for SARS-CoV-2 Envelop Protein [Severe acute respiratory syndrome coronavirus 2], YP_009724392	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00

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