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SARS-CoV-2 S Gene Tagged ORF (codon optimized)
CAT＃: VC102557
- TrueORF®
Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724390. Note: ORF is codon optimized
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CNY 8,864.00
| Cited in 1 publication. |
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>VC102557 representing NC_045512, codon optimized.
Due to the complexity of NC_045512, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTTGTGTTTCTCGTCCTGCTGCCTCTGGTCTCCTCACAGTGCGTGAATCTGACAACGAGAACTCAG CTCCCACCCGCATATACCAATTCTTTCACCCGGGGTGTGTACTACCCTGACAAAGTGTTCAGAAGCTCA GTACTTCATTCCACTCAGGACCTGTTTCTTCCATTTTTCAGCAATGTTACCTGGTTTCACGCCATCCAC GTATCCGGGACCAACGGCACGAAACGCTTTGATAACCCGGTCCTGCCATTCAACGACGGGGTCTATTTT GCTTCAACTGAAAAAAGCAACATTATCAGAGGCTGGATCTTCGGCACAACTCTGGACTCAAAGACTCAG TCATTGCTCATTGTCAATAACGCAACCAACGTTGTGATCAAGGTCTGTGAATTCCAGTTCTGCAACGAT CCGTTTCTGGGCGTATACTACCACAAGAACAACAAATCTTGGATGGAGAGTGAATTTCGAGTTTATTCC AGCGCGAACAACTGCACCTTCGAATACGTTAGTCAGCCCTTCCTCATGGACCTTGAGGGGAAACAAGGC AATTTTAAAAATCTGCGCGAATTCGTATTCAAAAACATCGATGGCTACTTTAAAATTTACTCCAAACAC ACTCCGATCAACCTGGTTAGGGACCTGCCCCAGGGCTTCTCCGCTCTGGAGCCTCTTGTGGACCTGCCC ATTGGAATTAATATCACCCGCTTTCAGACGCTGCTCGCCCTGCATCGGTCTTATCTGACTCCCGGCGAT TCCTCATCAGGTTGGACCGCTGGTGCTGCGGCCTATTACGTCGGATATTTGCAGCCTCGGACATTCCTT CTGAAATACAATGAGAATGGAACAATTACAGATGCTGTGGACTGTGCACTCGACCCGTTGTCCGAAACC AAATGCACCTTGAAAAGCTTTACTGTGGAAAAGGGCATCTATCAGACATCTAACTTCCGGGTTCAACCT ACCGAATCTATTGTCCGCTTCCCCAATATTACCAACCTTTGTCCCTTCGGTGAGGTATTCAACGCAACT CGGTTTGCCTCAGTCTACGCATGGAACCGCAAGAGGATTTCCAATTGCGTTGCCGATTATTCAGTCCTT TACAACTCCGCAAGCTTTTCCACGTTTAAATGCTACGGAGTCTCCCCAACAAAACTCAACGACCTGTGC TTTACCAATGTCTACGCAGACTCCTTTGTTATCCGCGGCGACGAGGTTAGGCAAATTGCCCCAGGGCAG ACTGGCAAGATCGCCGATTATAACTATAAGCTCCCCGATGACTTTACAGGCTGCGTGATCGCATGGAAC TCTAACAACCTCGATAGTAAGGTGGGGGGCAATTACAATTATCTTTACCGGCTGTTTCGAAAGTCAAAC CTTAAACCCTTCGAGCGCGATATTTCAACCGAAATTTATCAGGCCGGGAGCACACCATGCAACGGAGTG GAGGGTTTCAACTGCTACTTCCCACTGCAGTCTTACGGCTTCCAGCCCACCAATGGTGTGGGTTATCAG CCTTACAGAGTTGTTGTGCTCAGTTTCGAGCTCCTCCACGCCCCAGCTACCGTTTGCGGACCTAAGAAA AGCACAAACCTGGTCAAAAATAAATGCGTAAACTTCAACTTTAACGGTCTGACTGGTACCGGCGTTCTC ACCGAGTCCAATAAGAAATTCTTGCCATTTCAACAGTTTGGTAGAGACATCGCTGACACCACCGATGCT GTCCGGGATCCCCAGACCCTTGAGATTCTCGACATAACGCCATGCTCATTCGGGGGTGTCAGCGTTATC ACTCCGGGCACAAACACTAGCAATCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACTGAAGTACCC GTGGCCATTCACGCCGATCAGCTGACGCCAACCTGGAGAGTCTACTCAACAGGCTCTAACGTTTTTCAG ACAAGAGCCGGTTGTTTGATCGGAGCCGAACACGTAAATAATTCATATGAATGTGACATCCCAATCGGG GCCGGGATCTGCGCTAGTTACCAGACCCAGACTAACTCACCACGGAGAGCCAGAAGTGTAGCCTCCCAG TCAATAATCGCCTATACAATGTCTTTGGGCGCGGAAAATAGCGTGGCCTACTCCAATAATTCCATAGCC ATTCCCACTAACTTCACAATTTCCGTCACGACCGAGATCCTGCCGGTGTCAATGACAAAAACCTCTGTG GACTGCACAATGTATATATGTGGCGACAGTACCGAGTGTTCTAATCTCCTTTTGCAGTATGGGTCCTTC TGCACACAGCTGAATCGCGCTCTTACTGGTATCGCTGTGGAGCAAGATAAAAACACGCAGGAAGTGTTC GCTCAAGTGAAGCAGATTTATAAAACCCCTCCAATCAAAGATTTCGGCGGATTTAACTTCTCACAGATT CTCCCTGATCCAAGTAAACCCTCCAAAAGAAGTTTCATAGAGGACCTCCTGTTCAACAAGGTTACGCTG GCTGATGCTGGTTTCATCAAGCAGTATGGGGACTGCCTCGGCGACATAGCTGCTCGAGATTTGATCTGC GCCCAGAAATTCAATGGGCTGACAGTGCTGCCACCTCTCTTGACAGACGAAATGATTGCACAATATACT AGTGCCCTTTTGGCTGGCACCATCACATCCGGTTGGACTTTTGGTGCGGGAGCCGCACTCCAAATACCC TTCGCAATGCAGATGGCATACAGATTCAATGGCATTGGCGTCACCCAGAACGTTCTCTACGAAAACCAG AAACTGATAGCAAATCAGTTTAACAGCGCTATCGGCAAGATCCAGGACTCCCTCTCATCAACTGCCTCA GCCCTGGGCAAGCTCCAGGATGTGGTAAACCAAAACGCCCAGGCCCTCAACACTCTGGTCAAGCAGCTC AGTTCTAACTTCGGAGCCATCTCTAGTGTTCTCAATGACATCCTCTCTCGGCTGGACAAGGTGGAAGCA GAGGTGCAGATCGACCGCCTGATTACTGGACGCCTGCAGAGCCTCCAGACCTACGTAACCCAGCAACTC ATCCGGGCTGCCGAGATTAGGGCTTCCGCGAACCTCGCCGCCACAAAGATGAGCGAGTGCGTACTTGGA CAGAGCAAGAGAGTGGATTTTTGTGGCAAGGGGTATCATCTCATGTCCTTCCCTCAGAGCGCTCCTCAC GGTGTCGTATTTCTCCATGTGACTTATGTCCCCGCCCAAGAGAAAAACTTCACAACCGCTCCTGCTATC TGCCACGACGGCAAAGCCCATTTTCCTCGGGAGGGAGTGTTCGTCTCTAATGGGACTCACTGGTTTGTT ACGCAGAGGAACTTCTACGAGCCTCAGATCATCACAACAGATAACACCTTTGTGTCTGGTAACTGTGAC GTGGTCATCGGCATAGTAAATAACACCGTCTACGACCCACTGCAGCCTGAACTGGATAGCTTTAAGGAA GAGCTCGATAAGTACTTCAAAAACCACACAAGCCCGGATGTAGATCTTGGAGACATTAGTGGGATTAAC GCCAGCGTCGTTAACATACAAAAAGAGATTGACCGATTGAACGAAGTTGCCAAAAACCTGAATGAATCC CTGATCGATCTGCAGGAGCTCGGCAAGTACGAGCAGTATATTAAATGGCCCTGGTACATTTGGCTGGGC TTCATCGCGGGGCTCATCGCGATTGTGATGGTGACCATTATGCTGTGTTGCATGACCAGTTGCTGTAGC TGTCTCAAAGGATGCTGCTCATGTGGCAGCTGTTGCAAGTTCGACGAGGATGATTCCGAACCCGTACTG AAAGGCGTGAAACTCCATTATACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >VC102557 representing NC_045512
Blue=ORF Red=Cloning site Green=Tag(s) MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIH VSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCND PFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKH TPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFL LKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNAT RFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQ TGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGV EGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVL TESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVP VAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQ SIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSF CTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTL ADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIP FAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQL SSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLG QSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFV TQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGIN ASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCS CLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NC_045512 |
| ORF Size | 3819 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | YP_009724390 |
| RefSeq ORF | 3819 bp |
| Locus ID | 43740568 |
| MW | 141.2 kDa |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A Structurally Conserved RNA Element within SARS-CoV-2 ORF1a RNA and S mRNA Regulates Translation in Response to Viral S Protein-Induced Signaling in Human Lung Cells
,null,
Journal of Virology
,PubMed ID 34757848
[S Protein]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102555 | Myc-DDK-tagged ORF clone for SARS-CoV-2 nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724397 |
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| VC102556 | Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein extracellular domain [Severe acute respiratory syndrome coronavirus 2], YP_009724390 |
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| VC102558 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724391. Note: ORF is codon optimized |
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| VC102559 | Myc-DDK-tagged ORF clone for SARS-CoV-2 Membrane Glycoprotein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724393. Note: ORF is codon optimized |
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| VC102560 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724394 |
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| VC102561 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724395 |
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| VC102562 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724396 |
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|
| VC102563 | Myc-DDK-tagged ORF clone for SARS-CoV-2 Nucleocapsid Phosphoprotein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724397 |
CNY 3,656.00 |
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| VC102564 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009725255 |
CNY 3,800.00 |
|
| VC102565 | Myc-DDK-tagged ORF clone for SARS-CoV-2 Envelop Protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724392. Note: ORF is codon optimized |
CNY 1,200.00 |
|
| VC102566 | Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724390 |
CNY 8,864.00 |
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| VC102567 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], YP_009724391 |
CNY 2,400.00 |
|
| VC102568 | Myc-DDK-tagged ORF clone for SARS-CoV-2 Membrane Glycoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724393 |
CNY 2,400.00 |
|
| VC102569 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], YP_009724394 |
CNY 1,200.00 |
|
| VC102570 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], YP_009724395 |
CNY 1,200.00 |
|
| VC102571 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], YP_009724396 |
CNY 1,200.00 |
|
| VC102572 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], YP_009725255 |
CNY 1,200.00 |
|
| VC102573 | Myc-DDK-tagged ORF clone for SARS-CoV-2 Envelop Protein [Severe acute respiratory syndrome coronavirus 2], YP_009724392 |
CNY 1,200.00 |
|
| VC102574 | mRFP-tagged ORF clone for SARS-CoV-2 NSP4 [Severe acute respiratory syndrome coronavirus 2], YP_009725300.1 |
CNY 3,840.00 |
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| VC102575 | mRFP-tagged ORF clone for SARS-CoV-2 NSP5 [Severe acute respiratory syndrome coronavirus 2], YP_009725301.1 |
CNY 2,400.00 |
|
| VC102576 | mRFP-tagged ORF clone for SARS-CoV-2 NSP6 [Severe acute respiratory syndrome coronavirus 2], YP_009725302.1 |
CNY 2,400.00 |
|
| VC102577 | mRFP-tagged ORF clone for SARS-CoV-2 NSP7 [Severe acute respiratory syndrome coronavirus 2], YP_009725303.1 |
CNY 1,200.00 |
|
| VC102578 | mRFP-tagged ORF clone for SARS-CoV-2 NSP8 [Severe acute respiratory syndrome coronavirus 2], YP_009725304.1 |
CNY 2,400.00 |
|
| VC102579 | mRFP-tagged ORF clone for SARS-CoV-2 NSP9 [Severe acute respiratory syndrome coronavirus 2], YP_009725305.1 |
CNY 1,200.00 |
|
| VC102580 | mRFP-tagged ORF clone for SARS-CoV-2 NSP10 [Severe acute respiratory syndrome coronavirus 2], YP_009725306.1 |
CNY 1,200.00 |
|
| VC102581 | mRFP-tagged ORF clone for SARS-CoV-2 NSP14 [Severe acute respiratory syndrome coronavirus 2], YP_009725309.1 |
CNY 4,040.00 |
|
| VC102582 | mRFP-tagged ORF clone for SARS-CoV-2 ORF3a [Severe acute respiratory syndrome coronavirus 2], YP_009724391.1 |
CNY 2,400.00 |
|
| VC102583 | mRFP-tagged ORF clone for SARS-CoV-2 M protein [Severe acute respiratory syndrome coronavirus 2], YP_009724393.1 |
CNY 2,400.00 |
|
| VC102584 | mRFP-tagged ORF clone for SARS-CoV-2 ORF9b [Severe acute respiratory syndrome coronavirus 2], YP_009724397.2 |
CNY 1,200.00 |
|
| VC102585 | mRFP-tagged ORF clone for SARS-CoV-2 ORF9c [Severe acute respiratory syndrome coronavirus 2], YP_009724397.2 |
CNY 1,200.00 |
|
| VC102586 | mRFP-tagged ORF clone for SARS-CoV-2 ORF10 [Severe acute respiratory syndrome coronavirus 2], YP_009725255.1 |
CNY 1,200.00 |
|
| VC102587 | Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein mutant (D614G) [Severe acute respiratory syndrome coronavirus 2], YP_009724390 |
CNY 8,864.00 |
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| VC202557 | Native cDNA clone for SARS-CoV-2 surface glycoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724390 |
CNY 8,872.00 |
|
| VC202558 | Native cDNA clone for SARS-CoV-2 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], YP_009724391 |
CNY 2,400.00 |
|
| VC202559 | Native cDNA clone for SARS-CoV-2 Membrane Glycoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724393 |
CNY 2,400.00 |
|
| VC202560 | Native cDNA clone for SARS-CoV-2 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], YP_009724394 |
CNY 3,800.00 |
|
| VC202561 | Native cDNA clone for SARS-CoV-2 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], YP_009724395 |
CNY 3,800.00 |
|
| VC202562 | Native cDNA clone for SARS-CoV-2 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], YP_009724396 |
CNY 3,800.00 |
|
| VC202563 | Native cDNA clone for SARS-CoV-2 Nucleocapsid Phosphoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724397 |
CNY 3,656.00 |
|
| VC202564 | Native cDNA clone for SARS-CoV-2 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], YP_009725255 |
CNY 3,800.00 |
|
| VC202565 | Native cDNA clone for SARS-CoV-2 Envelop Protein [Severe acute respiratory syndrome coronavirus 2], YP_009724392 |
CNY 3,800.00 |
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