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NS5A protein (NC_004102) Virus Tagged ORF Clone
CAT＃: VC102223
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for NS5A protein [Hepatitis C virus], codon optimized for human cell expression, NP_751927
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CNY 3,656.00
CNY 5,510.00
| Cited in 1 publication. |
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102223 represents NCBI reference of NP_751927 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGCTCTTGGCTCAGGGACATCTGGGACTGGATCTGTGAAGTTTTGTCAGACTTTAAAACATGGC TCAAAGCCAAACTGATGCCTCAACTGCCAGGGATTCCATTTGTAAGCTGCCAGCGGGGTTATAGGGGCGT ATGGCGGGGAGACGGAATCATGCACACCCGGTGTCACTGTGGAGCAGAAATTACGGGTCATGTGAAAAAT GGCACAATGAGGATAGTTGGACCTCGCACTTGTAGAAATATGTGGTCAGGGACTTTCCCCATTAATGCAT ACACTACCGGTCCATGCACACCGCTGCCAGCCCCAAATTACAAGTTCGCGCTGTGGCGGGTAAGCGCTGA AGAGTACGTGGAAATTCGGCGGGTTGGTGACTTCCACTACGTGTCTGGCATGACAACCGACAACCTGAAG TGCCCCTGTCAGATTCCATCTCCCGAATTCTTCACAGAACTTGATGGGGTCCGGCTCCACAGATTCGCAC CTCCGTGCAAACCACTGCTGAGAGAAGAAGTTTCTTTTCGGGTTGGCCTTCACGAGTATCCCGTTGGGAG TCAGCTTCCCTGTGAACCGGAACCTGACGTGGCCGTGCTGACAAGCATGCTTACCGACCCCTCTCATATC ACCGCCGAAGCCGCTGGGCGGAGACTGGCACGAGGGAGTCCCCCATCAATGGCTAGCAGTTCCGCCAGCC AACTGAGTGCCCCATCACTTAAAGCAACCTGCACCGCCAATCACGATTCTCCCGACGCCGAGCTGATCGA GGCCAATCTGCTCTGGCGGCAGGAAATGGGAGGCAACATCACTAGGGTGGAGTCTGAGAACAAGGTCGTA ATTCTGGACTCATTTGACCCGCTGGTTGCTGAAGAAGACGAGAGAGAGGTCTCTGTGCCCGCTGAAATTC TCCGAAAGAGCCGGCGGTTTGCCAGAGCCCTCCCTGTTTGGGCAAGACCTGATTATAATCCGCCATTGGT GGAGACTTGGAAAAAGCCTGACTACGAGCCTCCGGTTGTCCACGGTTGCCCTCTTCCACCACCCCGCTCA CCTCCAGTGCCACCTCCCCGCAAAAAAAGAACCGTCGTGCTCACTGAATCAACCCTTTCTACTGCCCTTG CAGAGTTGGCGACTAAGTCCTTCGGAAGTTCAAGCACTAGTGGCATCACCGGCGACAATACTACAACTTC ATCCGAGCCAGCGCCTTCAGGCTGCCCACCCGACTCCGACGTGGAATCCTACAGCTCCATGCCGCCCCTG GAAGGGGAACCAGGAGATCCTGATCTCTCTGATGGTTCCTGGTCCACTGTGTCTTCAGGAGCCGATACCG AGGACGTGGTCTGTTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102223 representing NP_751927
Red=Cloning sites Green=Tags MSGSWLRDIWDWICEVLSDFKTWLKAKLMPQLPGIPFVSCQRGYRGVWRGDGIMHTRCHCGAEITGHVKN GTMRIVGPRTCRNMWSGTFPINAYTTGPCTPLPAPNYKFALWRVSAEEYVEIRRVGDFHYVSGMTTDNLK CPCQIPSPEFFTELDGVRLHRFAPPCKPLLREEVSFRVGLHEYPVGSQLPCEPEPDVAVLTSMLTDPSHI TAEAAGRRLARGSPPSMASSSASQLSAPSLKATCTANHDSPDAELIEANLLWRQEMGGNITRVESENKVV ILDSFDPLVAEEDEREVSVPAEILRKSRRFARALPVWARPDYNPPLVETWKKPDYEPPVVHGCPLPPPRS PPVPPPRKKRTVVLTESTLSTALAELATKSFGSSSTSGITGDNTTTSSEPAPSGCPPDSDVESYSSMPPL EGEPGDPDLSDGSWSTVSSGADTEDVVCC TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_004102 |
| ORF Size | 1347 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_004102.1, NP_751927 |
| RefSeq ORF | 1347 bp |
| MW | 49.2 kDa |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Identification of cleavage of NS5A of C-strain classical swine fever virus.
,null,
Archives of virology
,PubMed ID 27766426
[NS5A protein]
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Documents
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102214 | Myc-DDK-tagged ORF clone of viral ORF for protein F [Hepatitis C virus], codon optimized for human cell expression, NP_803170 |
CNY 1,200.00 |
|
| VC102215 | Myc-DDK-tagged ORF clone of viral ORF for core protein [Hepatitis C virus], codon optimized for human cell expression, NP_751919 |
CNY 3,600.00 |
|
| VC102216 | Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751920 |
CNY 2,400.00 |
|
| VC102217 | Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751921 |
CNY 3,656.00 |
|
| VC102218 | Myc-DDK-tagged ORF clone of viral ORF for p7 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751922 |
CNY 1,200.00 |
|
| VC102219 | Myc-DDK-tagged ORF clone of viral ORF for NS2 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751923 |
CNY 2,400.00 |
|
| VC102220 | Myc-DDK-tagged ORF clone of viral ORF for NS3 protease/helicase' [Hepatitis C virus], codon optimized for human cell expression, NP_803144 |
CNY 4,704.00 |
|
| VC102221 | Myc-DDK-tagged ORF clone of viral ORF for NS4A protein [Hepatitis C virus], codon optimized for human cell expression, NP_751925 |
CNY 1,200.00 |
|
| VC102222 | Myc-DDK-tagged ORF clone of viral ORF for NS4B protein [Hepatitis C virus], codon optimized for human cell expression, NP_751926 |
CNY 2,400.00 |
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