NS3 protease/helicase' (NC_004102) Virus Tagged ORF Clone

CAT#: VC102220

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for NS3 protease/helicase' [Hepatitis C virus], codon optimized for human cell expression, NP_803144



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CNY 4,704.00

CNY 7,600.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102220 represents NCBI reference of NP_803144 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCACCTATTACAGCATACGCTCAACAAACGCGGGGGCTGCTCGGGTGCATAATTACAAGTCTGACTG
GGCGGGATAAAAATCAGGTGGAAGGCGAAGTGCAGATAGTAAGTACCGCTACTCAAACTTTTCTCGCCAC
ATGTATAAACGGCGTGTGCTGGACGGTTTACCACGGAGCAGGAACCAGGACTATTGCTTCACCAAAAGGT
CCTGTTATTCAAATGTACACAAATGTCGATCAGGATCTCGTAGGTTGGCCCGCACCTCAAGGGAGCAGGT
CACTGACCCCGTGTACCTGCGGGAGTTCCGATCTCTACCTTGTGACACGACACGCTGATGTAATTCCCGT
CCGCAGGCGGGGGGATAGCAGAGGGAGTCTGCTGTCTCCAAGGCCGATCTCTTATTTGAAGGGCTCTTCC
GGCGGCCCACTGCTGTGCCCAGCAGGCCATGCGGTAGGTCTGTTTCGAGCCGCCGTGTGTACCAGGGGCG
TAGCGAAAGCTGTCGACTTCATACCTGTGGAGAATCTCGAGACGACTATGCGCTCACCAGTTTTCACCGA
TAACAGCAGCCCGCCAGCCGTGCCGCAGTCCTTCCAAGTGGCTCACCTGCACGCCCCCACCGGATCCGGC
AAGTCCACCAAAGTCCCCGCTGCTTACGCAGCCCAAGGCTACAAGGTCTTGGTACTGAATCCTTCAGTGG
CTGCCACTCTCGGCTTTGGCGCCTACATGTCTAAGGCACACGGAGTTGATCCTAATATTCGAACCGGTGT
ACGCACCATCACCACTGGCTCCCCTATCACTTATAGTACATACGGAAAATTTCTGGCCGACGGCGGATGT
TCCGGGGGCGCCTATGATATCATAATATGCGACGAGTGCCACAGTACTGATGCTACCAGTATTCTCGGTA
TCGGCACAGTCCTGGATCAGGCGGAAACAGCGGGTGCCAGACTGGTCGTTCTGGCCACGGCTACCCCACC
TGGATCTGTGACAGTGTCTCACCCAAACATTGAGGAAGTGGCTCTGAGCACCACCGGTGAAATCCCGTTT
TATGGTAAAGCTATCCCACTCGAGGTGATCAAAGGGGGCCGACACTTGATTTTTTGTCACAGTAAGAAAA
AGTGTGATGAACTCGCGGCAAAACTGGTAGCTCTGGGGATTAATGCCGTGGCTTACTATAGAGGTCTGGA
CGTGAGTGTGATTCCGACCAGCGGCGACGTCGTAGTTGTGAGTACAGATGCCCTCATGACTGGCTTTACA
GGAGACTTTGATTCCGTTATTGATTGTAACACATGTGTGACCCAGACCGTGGACTTCAGCCTGGACCCCA
CGTTCACAATTGAAACTACAACATTGCCCCAAGACGCCGTGTCCAGGACCCAGAGGAGAGGAAGGACAGG
CCGCGGAAAACCTGGAATCTATAGATTCGTAGCCCCCGGGGAACGCCCATCCGGCATGTTTGATTCTTCC
GTGTTGTGTGAGTGCTATGACGCTGGCTGCGCTTGGTACGAGCTTACTCCTGCAGAGACCACTGTCAGGT
TGAGGGCCTACATGAATACACCAGGTTTGCCCGTATGCCAGGACCACCTGGAGTTTTGGGAAGGCGTCTT
CACCGGCCTGACTCATATTGACGCTCATTTTCTGTCTCAGACAAAGCAATCTGGTGAGAATTTCCCTTAC
CTGGTTGCCTATCAAGCAACGGTTTGTGCTAGGGCGCAGGCCCCCCCGCCCTCATGGGACCAAATGTGGA
AATGTCTCATTAGGCTCAAGCCCACATTGCACGGACCCACACCTCTCCTGTATCGGCTCGGTGCAGTCCA
GAACGAAGTTACACTGACCCACCCCATCACCAAGTATATCATGACCTGTATGAGCGCCGACCTGGAAGTG
GTTACT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102220 representing NP_803144
Red=Cloning sites Green=Tags

MAPITAYAQQTRGLLGCIITSLTGRDKNQVEGEVQIVSTATQTFLATCINGVCWTVYHGAGTRTIASPKG
PVIQMYTNVDQDLVGWPAPQGSRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSS
GGPLLCPAGHAVGLFRAAVCTRGVAKAVDFIPVENLETTMRSPVFTDNSSPPAVPQSFQVAHLHAPTGSG
KSTKVPAAYAAQGYKVLVLNPSVAATLGFGAYMSKAHGVDPNIRTGVRTITTGSPITYSTYGKFLADGGC
SGGAYDIIICDECHSTDATSILGIGTVLDQAETAGARLVVLATATPPGSVTVSHPNIEEVALSTTGEIPF
YGKAIPLEVIKGGRHLIFCHSKKKCDELAAKLVALGINAVAYYRGLDVSVIPTSGDVVVVSTDALMTGFT
GDFDSVIDCNTCVTQTVDFSLDPTFTIETTTLPQDAVSRTQRRGRTGRGKPGIYRFVAPGERPSGMFDSS
VLCECYDAGCAWYELTPAETTVRLRAYMNTPGLPVCQDHLEFWEGVFTGLTHIDAHFLSQTKQSGENFPY
LVAYQATVCARAQAPPPSWDQMWKCLIRLKPTLHGPTPLLYRLGAVQNEVTLTHPITKYIMTCMSADLEV
VT

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_004102
ORF Size 1896 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_004102.1, NP_803144
RefSeq ORF 1896 bp
MW 67.4 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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