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reverse transcriptase p51 subunit (NC_001802) Virus Tagged ORF Clone
CAT＃: VC101737
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for reverse transcriptase p51 subunit [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_789739
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CNY 3,656.00
CNY 5,420.00
| Cited in 1 publication. |
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101737 represents NCBI reference of NP_789739 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAATTAGTCCAATTGAAACAGTTCCAGTTAAGCTTAAACCAGGAATGGACGGCCCTAAGGTTAAGC AGTGGCCACTGACCGAGGAAAAAATCAAAGCTCTGGTGGAAATTTGCACCGAGATGGAGAAGGAGGGAAA AATTTCAAAGATTGGTCCTGAGAACCCGTATAACACTCCCGTATTTGCTATTAAGAAAAAAGACTCTACA AAATGGAGGAAACTGGTGGATTTTCGAGAACTCAATAAAAGGACCCAGGATTTCTGGGAGGTGCAGCTCG GGATCCCACACCCAGCGGGCTTGAAAAAGAAGAAGAGTGTAACTGTGCTTGACGTGGGCGACGCATATTT TTCAGTACCACTCGATGAAGACTTCAGGAAGTACACGGCGTTTACCATTCCTTCAATCAACAATGAGACA CCGGGGATCCGGTACCAGTATAACGTCCTCCCCCAAGGGTGGAAAGGGTCCCCTGCCATATTTCAGTCTA GCATGACAAAAATTCTCGAACCATTTAGGAAACAGAATCCAGACATCGTTATCTATCAGTATATGGATGA TCTGTACGTTGGCAGCGACCTTGAGATTGGACAACACCGAACCAAAATTGAGGAGCTGCGACAGCATCTG CTGAGATGGGGCCTCACTACTCCAGACAAAAAGCATCAGAAAGAGCCTCCGTTTCTGTGGATGGGGTATG AGCTGCACCCAGACAAATGGACTGTTCAGCCCATTGTGCTCCCGGAAAAAGATTCTTGGACCGTTAATGA TATCCAGAAACTTGTAGGGAAGCTGAACTGGGCATCCCAGATCTATCCCGGGATCAAGGTACGCCAGCTC TGCAAACTCCTCCGCGGCACAAAGGCACTTACTGAAGTGATCCCACTGACGGAAGAGGCGGAACTCGAGC TCGCAGAAAATCGAGAGATCCTGAAGGAGCCCGTTCACGGCGTGTACTACGATCCTTCCAAGGACCTGAT TGCAGAGATTCAGAAGCAGGGACAGGGACAGTGGACCTATCAGATCTACCAGGAGCCTTTTAAGAATCTC AAGACAGGAAAATACGCCCGAATGCGAGGAGCCCACACCAATGATGTTAAGCAACTGACCGAGGCGGTCC AGAAGATCACCACAGAAAGCATCGTGATCTGGGGCAAAACTCCGAAGTTCAAACTCCCGATCCAGAAGGA GACTTGGGAAACTTGGTGGACCGAGTACTGGCAGGCCACTTGGATACCGGAGTGGGAGTTCGTTAACACA CCCCCTCTCGTAAAGCTGTGGTACCAGTTGGAGAAGGAGCCGATCGTCGGCGCTGAAACTTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101737 representing NP_789739
Red=Cloning sites Green=Tags MPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDST KWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDEDFRKYTAFTIPSINNET PGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHL LRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQL CKLLRGTKALTEVIPLTEEAELELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNL KTGKYARMRGAHTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNT PPLVKLWYQLEKEPIVGAETF TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001802 |
| ORF Size | 1323 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001802.1, NP_789739 |
| RefSeq ORF | 1323 bp |
| UniProt ID | P04585 |
| MW | 51.5 kDa |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The circadian clock components BMAL1 and REV-ERBα regulate flavivirus replication
,null,
Nature Communications
,PubMed ID 30670689
[reverse transcriptase p51 subunit]
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Documents
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Resources
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| VC101724 | Myc-DDK-tagged ORF clone of viral ORF for matrix [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579876 |
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| VC101725 | Myc-DDK-tagged ORF clone of viral ORF for capsid [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579880 |
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| VC101726 | Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579881 |
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| VC101727 | Myc-DDK-tagged ORF clone of viral ORF for p2 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579882 |
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| VC101728 | Myc-DDK-tagged ORF clone of viral ORF for p6 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579883 |
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| VC101729 | Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579893 |
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| VC101730 | Myc-DDK-tagged ORF clone of viral ORF for Envelope transmembrane glycoprotein gp41 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579895 |
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| VC101732 | Myc-DDK-tagged ORF clone of viral ORF for retropepsin [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_705926 |
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| VC101735 | Myc-DDK-tagged ORF clone of viral ORF for p1 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_787042 |
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| VC101736 | Myc-DDK-tagged ORF clone of viral ORF for Gag-Pol Transframe peptide [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_787043 |
CNY 1,200.00 |
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| VC101739 | Myc-DDK-tagged ORF clone of viral ORF for Envelope surface glycoprotein gp120 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579894 |
CNY 3,656.00 |
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