HCoV-HKU1 nsp14 Gene Tagged ORF Clone

CAT＃: VC100586

TrueORF^®

Myc-DDK-tagged ORF clone for nsp14 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460021

Datasheet

SDS

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CNY 6,370.00

货期*

4周

规格

Product images

经常一起买 (4)

PS100001

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CC100003

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CNY 1,280.00

TA592569

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 600.00

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TA50011-100

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

CNY 1,050.00

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Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC100586 represents NCBI reference of YP_460021 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTACCACTAACCTCTTCAAGGACTGCTCCAAGAGCTGCCTGGGATATCATCCTGCACATGCACCTT CTTTTCTGGCTGTCGACGATAAGTATAAAGTTAACGAAAACCTGGCCGTGAACCTGAACATTTGTGAACC CGTCCTGACTTATTCAAGGTTGATTAGCTTGATGGGCTTCAAGCTGGACCTCACACTCGACGGCTACTCT AAACTCTTCATTACCAAGGACGAGGCCATCAAGCGGGTGAGGGGTTGGGTGGGCTTTGACGTCGAGGGGG CGCACGCGACACGAGAAAACATCGGAACTAACTTTCCACTCCAGATAGGTTTCTCTACCGGAGTGGATTT CGTGGTGGAGGCAACCGGTCTGTTTGCAGAAAGGGATTGTTACACGTTCAAAAAGACAGTCGCCAAGGCT CCCCCCGGTGAGAAGTTCAAGCACCTTATTCCACTTATGTCCAAGGGACAAAAGTGGGACATCGTGCGCA TCAGAATCGTTCAGATGCTGTCAGATTACCTGCTGGATTTGTCAGACTCCGTGGTCTTTATCACCTGGTC AGCCTCCTTCGAACTTACTTGCTTGCGGTACTTCGCCAAGCTCGGGCGAGAGCTGAACTGCAACGTATGC TCAAACCGCGCCACATGTTACAACAGCAGAACAGGCTACTATGGATGCTGGCGCCATAGCTATACCTGTG ATTACGTATATAACCCGCTGATAGTTGATATCCAGCAGTGGGGTTATACCGGAAGTCTGACCTCAAACCA CGATATCATCTGTAATGTCCACAAGGGCGCACACGTAGCGTCCGCTGATGCCATTATGACGAGGTGTTTG GCAATCTATGATTGCTTCTGCAAGAGCGTAAATTGGAACTTGGAATACCCAATTATCAGTAACGAGGTGA GCATCAATACCTCATGCCGCCTGCTGCAAAGAGTGATGCTGAAAGCTGCTATGCTCTGTAATAGGTATAA TTTGTGTTATGATATCGGAAACCCGAAAGGACTGGCCTGTGTTAAGGATTACGAGTTTAAGTTCTACGAT GCCTTCCCCGTCGCTAAATCCGTGAAGCAGCTTTTTTATGTCTACGACGTGCACAAAGATAATTTCAAGG ATGGGCTCTGCATGTTCTGGAACTGCAACGTCGATAAGTATCCTAGCAACAGCATCGTGTGTCGGTTCGA CACCCGGGTCCTGAATAAATTGAATCTGCCCGGATGTAACGGCGGTAGCCTGTATGTAAATAAACACGCA TTTCACACAAACCCATTTACCAGAACCGTCTTTGAGAATCTGAAGCCCATGCCATTTTTCTACTACTCCG ATACACCATGTGTTTACGTGGACGGGTTGGAGTCCAAGCAGGTGGATTATGTGCCACTGCGGTCAGCCAC TTGTATTACCCGATGTAACCTCGGAGGAGCTGTGTGTAGTAAGCACGCTGAGGAATACTGTAACTACCTG GAGAGCTATAACATCGTGACTACAGCCGGCTTTACCTTCTGGGTGTACAAGAACTTCGATTTTTACAATT TGTGGAACACGTTTACAACACTCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC100586 representing YP_460021 Red=Cloning sites Green=Tags MCTTNLFKDCSKSCLGYHPAHAPSFLAVDDKYKVNENLAVNLNICEPVLTYSRLISLMGFKLDLTLDGYS KLFITKDEAIKRVRGWVGFDVEGAHATRENIGTNFPLQIGFSTGVDFVVEATGLFAERDCYTFKKTVAKA PPGEKFKHLIPLMSKGQKWDIVRIRIVQMLSDYLLDLSDSVVFITWSASFELTCLRYFAKLGRELNCNVC SNRATCYNSRTGYYGCWRHSYTCDYVYNPLIVDIQQWGYTGSLTSNHDIICNVHKGAHVASADAIMTRCL AIYDCFCKSVNWNLEYPIISNEVSINTSCRLLQRVMLKAAMLCNRYNLCYDIGNPKGLACVKDYEFKFYD AFPVAKSVKQLFYVYDVHKDNFKDGLCMFWNCNVDKYPSNSIVCRFDTRVLNKLNLPGCNGGSLYVNKHA FHTNPFTRTVFENLKPMPFFYYSDTPCVYVDGLESKQVDYVPLRSATCITRCNLGGAVCSKHAEEYCNYL ESYNIVTTAGFTFWVYKNFDFYNLWNTFTTLQ TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	NC_006577
ORF Size	1566 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NC_006577.2, YP_460021
RefSeq ORF	1566 bp
MW	59.7 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC100570	Myc-DDK-tagged ORF clone for hemagglutinin-esterase glycoprotein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173237	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 4,560.00
VC100572	Myc-DDK-tagged ORF clone for non-structural protein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173239	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100573	Myc-DDK-tagged ORF clone for small membrane protein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173240	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100574	Myc-DDK-tagged ORF clone for membrane glycoprotein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173241	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC100575	Myc-DDK-tagged ORF clone for nucleocapsid phosphoprotein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173242	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,488.00
VC100576	Myc-DDK-tagged ORF clone for nucleocapsid phosphoprotein 2 [Human coronavirus HKU1], codon optimized for human cell expression, YP_173243	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100577	Myc-DDK-tagged ORF clone for nsp1 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460018	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100578	Myc-DDK-tagged ORF clone for nsp2 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459934	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,030.00
VC100579	Myc-DDK-tagged ORF clone for nsp6 (hydrophobic domain) [Human coronavirus HKU1], codon optimized for human cell expression, YP_460019	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100580	Myc-DDK-tagged ORF clone for nsp5 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459936	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100581	Myc-DDK-tagged ORF clone for nsp7 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459938	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100582	Myc-DDK-tagged ORF clone for nsp8 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460020	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100583	Myc-DDK-tagged ORF clone for nsp9 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459943	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100584	Myc-DDK-tagged ORF clone for nsp10 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459939	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100585	Myc-DDK-tagged ORF clone for nsp13 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459942	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,220.00
VC100587	Myc-DDK-tagged ORF clone for nsp15 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460022	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,470.00
VC100588	Myc-DDK-tagged ORF clone for nsp16 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460023	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100591	Myc-DDK-tagged ORF clone for nsp4 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459935	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,080.00

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