United States
Germany
Japan
United Kingdom
China
HCoV-HKU1 N Gene Gene Tagged ORF Clone
CAT＃: VC100575
- TrueORF®
Myc-DDK-tagged ORF clone for nucleocapsid phosphoprotein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173242
View other clones from "HCoV-HKU1" (18)
Need custom modification / cloning service?
Get a free quote
CNY 5,488.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC100575 represents NCBI reference of YP_173242 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTACACCCCCGGCCATTACGCCGGCAGCCGGAGCTCCTCCGGAAATCGGTCCGGCATCCTCAAGA AGACCAGCTGGGCCGACCAGAGCGAGCGGAACTACCAGACCTTCAATAGAGGACGGAAGACCCAGCCCAA GTTCACCGTCTCCACCCAGCCCCAGGGGAACACAATTCCCCACTACAGCTGGTTCAGCGGGATCACCCAA TTTCAAAAAGGCCGGGACTTCAAGTTTAGCGACGGCCAGGGCGTGCCCATCGCCTTCGGCGTGCCTCCCA GCGAAGCCAAGGGGTACTGGTATCGGCATAGCCGGCGGAGCTTCAAGACCGCCGACGGGCAGCAGAAGCA GCTCCTGCCTCGGTGGTACTTCTACTACCTCGGCACAGGCCCCTACGCTAATGCCAGCTACGGCGAGAGC CTGGAGGGCGTGTTTTGGGTCGCAAACCACCAGGCCGACACCTCCACTCCCAGCGACGTGAGCTCCCGGG ACCCCACCACCCAGGAGGCCATCCCCACCCGGTTTCCCCCCGGCACCATCCTGCCCCAAGGCTACTACGT GGAGGGGTCCGGCCGGTCCGCCAGCAATTCCCGGCCCGGCAGCCGGTCCCAGAGCCGGGGACCCAACAAC CGGTCCCTGAGCCGGTCCAACAGCAACTTCCGGCACAGCGACAGCATCGTGAAGCCCGATATGGCAGACG AGATCGCCAACCTGGTGCTGGCCAAGCTCGGCAAGGACAGCAAGCCACAGCAGGTCACCAAGCAGAATGC CAAGGAAATCCGGCATAAGATTCTGACCAAGCCCCGGCAAAAGCGGACCCCAAACAAGCACTGCAACGTG CAGCAGTGCTTCGGCAAGCGGGGGCCCAGCCAAAACTTCGGGAACGCCGAGATGCTCAAGCTGGGCACCA ATGACCCCCAGTTCCCCATCCTCGCCGAGCTGGCCCCCACCCCCGGCGCCTTCTTCTTCGGCTCCAAGCT CGACCTGGTGAAGCGGGATAGCGAGGCCGACAGCCCCGTGAAGGACGTCTTTGAGCTGCATTACAGCGGG AGCATCCGGTTCGACAGCACCCTGCCCGGCTTCGAGACCATCATGAAGGTGCTGGAGGAGAACCTCAACG CCTACGTGAACAGCAACCAGAACACCGACTCCGACAGCCTGTCCAGCAAGCCCCAGAGAAAGCGGGGCGT GAAACAACTGCCCGAGCAGTTCGACAGCCTGAACCTGTCCGCCGGCACCCAGCACATCTCCAACGACTTC ACACCCGAAGATCACAGCCTCCTGGCCACCCTCGACGACCCATACGTCGAGGACAGCGTCGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100575 representing YP_173242
Red=Cloning sites Green=Tags MSYTPGHYAGSRSSSGNRSGILKKTSWADQSERNYQTFNRGRKTQPKFTVSTQPQGNTIPHYSWFSGITQ FQKGRDFKFSDGQGVPIAFGVPPSEAKGYWYRHSRRSFKTADGQQKQLLPRWYFYYLGTGPYANASYGES LEGVFWVANHQADTSTPSDVSSRDPTTQEAIPTRFPPGTILPQGYYVEGSGRSASNSRPGSRSQSRGPNN RSLSRSNSNFRHSDSIVKPDMADEIANLVLAKLGKDSKPQQVTKQNAKEIRHKILTKPRQKRTPNKHCNV QQCFGKRGPSQNFGNAEMLKLGTNDPQFPILAELAPTPGAFFFGSKLDLVKRDSEADSPVKDVFELHYSG SIRFDSTLPGFETIMKVLEENLNAYVNSNQNTDSDSLSSKPQRKRGVKQLPEQFDSLNLSAGTQHISNDF TPEDHSLLATLDDPYVEDSVA TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_006577 |
| ORF Size | 1323 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_006577.2, YP_173242 |
| RefSeq ORF | 1323 bp |
| Locus ID | 3200423 |
| MW | 49.1 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC100570 | Myc-DDK-tagged ORF clone for hemagglutinin-esterase glycoprotein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173237 |
CNY 3,656.00 |
|
| VC100572 | Myc-DDK-tagged ORF clone for non-structural protein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173239 |
CNY 3,800.00 |
|
| VC100573 | Myc-DDK-tagged ORF clone for small membrane protein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173240 |
CNY 3,800.00 |
|
| VC100574 | Myc-DDK-tagged ORF clone for membrane glycoprotein [Human coronavirus HKU1], codon optimized for human cell expression, YP_173241 |
CNY 2,400.00 |
|
| VC100576 | Myc-DDK-tagged ORF clone for nucleocapsid phosphoprotein 2 [Human coronavirus HKU1], codon optimized for human cell expression, YP_173243 |
CNY 3,800.00 |
|
| VC100577 | Myc-DDK-tagged ORF clone for nsp1 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460018 |
CNY 3,800.00 |
|
| VC100578 | Myc-DDK-tagged ORF clone for nsp2 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459934 |
CNY 7,030.00 |
|
| VC100579 | Myc-DDK-tagged ORF clone for nsp6 (hydrophobic domain) [Human coronavirus HKU1], codon optimized for human cell expression, YP_460019 |
CNY 3,800.00 |
|
| VC100580 | Myc-DDK-tagged ORF clone for nsp5 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459936 |
CNY 3,800.00 |
|
| VC100581 | Myc-DDK-tagged ORF clone for nsp7 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459938 |
CNY 3,800.00 |
|
| VC100582 | Myc-DDK-tagged ORF clone for nsp8 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460020 |
CNY 3,800.00 |
|
| VC100583 | Myc-DDK-tagged ORF clone for nsp9 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459943 |
CNY 3,800.00 |
|
| VC100584 | Myc-DDK-tagged ORF clone for nsp10 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459939 |
CNY 3,800.00 |
|
| VC100585 | Myc-DDK-tagged ORF clone for nsp13 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459942 |
CNY 7,220.00 |
|
| VC100586 | Myc-DDK-tagged ORF clone for nsp14 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460021 |
CNY 6,370.00 |
|
| VC100587 | Myc-DDK-tagged ORF clone for nsp15 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460022 |
CNY 4,470.00 |
|
| VC100588 | Myc-DDK-tagged ORF clone for nsp16 [Human coronavirus HKU1], codon optimized for human cell expression, YP_460023 |
CNY 3,800.00 |
|
| VC100591 | Myc-DDK-tagged ORF clone for nsp4 [Human coronavirus HKU1], codon optimized for human cell expression, YP_459935 |
CNY 6,080.00 |
其它N Protein产品
