BAZ2A (NM_001300905) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	TIP5; WALp3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300905, the custom clone sequence may differ by one or more nucleotides ATGGAGGCAAACGACCATTTTAACTTTACTGGCCTTCCCCCTGCACCTGCTGCCTCAGGACTGAAACCCT CTCCTTCCTCAGGGGAGGGCCTCTACACTAACGGGTCTCCCATGAACTTCCCCCAGCAAGGGAAAAGTTT GAATGGGGATGTGAATGTTAATGGCTTATCTACTGTATCTCACACTACTACTTCAGGGATTTTGAACTCT GCTCCCCACTCCTCCAGCACCTCACACCTCCATCACCCCAGCGTGGCCTACGACTGTCTCTGGAACTACT CACAGTACCCATCTGCCAATCCTGGCAGCAACCTCAAGGACCCACCCCTTCTCTCCCAGTTCTCGGGGGG ACAATACCCACTCAACGGCATCCTTGGGGGCAGCCGGCAACCTTCATCCCCAAGTCATAACACTAACCTT CGGGCTGGGAGCCAAGAGTTCTGGGCCAACGGTACCCAGAGTCCCATGGGGCTTAACTTTGATTCACAAG AACTGTATGATTCCTTTCCTGACCAGAATTTTGAGGTGATGCCCAATGGACCCCCTAGTTTTTTCACCTC CCCACAGACTTCTCCTATGTTGGGATCTAGCATTCAAACCTTTGCACCCTCCCAGGAGGTAGGCAGTGGT ATCCATCCTGATGAGGCAGCAGAAAAGGAGATGACTTCAGTTGTGGCAGAGAATGGCACTGGCTTGGTAG GCAGCTTGGAGCTGGAAGAAGAGCAGCCAGAACTGAAGATGTGTGGCTACAATGGCTCTGTCCCTTCTGT GGAATCGTTACACCAAGAGGTCTCAGTCCTGGTCCCTGACCCCACAGTGAGCTGTTTAGATGATCCTTCA CATCTTCCTGATCAACTGGAAGACACTCCAATCCTCAGTGAAGACTCTCTGGAGCCCTTCAACTCTCTGG CACCAGAGCCAGTGAGTGGAGGACTATATGGTATTGATGACACGGAGCTGATGGGTGCAGAGGACAAGCT GCCTCTTGAGGACAGCCCTGTGATTTCTGCCCTTGATTGCCCTTCCCTCAATAATGCTACTGCCTTCAGT CTCCTGGCAGATGATAGTCAAACATCAACCTCTATCTTTGCCAGTCCCACCTCTCCACCTGTCCTAGGGG AGTCTGTCCTGCAAGATAACAGCTTTGACCTGAATAATGGTAGTGACGCTGAACAGGAAGAAATGGAAAC TCAATCTTCAGACTTCCCACCATCCCTGACCCAGCCAGCTCCTGATCAGTCATCCACTATTCAGCTACAT CCAGCAACCTCACCAGCAGTCTCGCCAACAACCTCCCCAGCAGTCTCCCTAGTGGTTTCTCCAGCAGCCT CCCCAGAAATCTCTCCAGAAGTTTGTCCCGCAGCTTCTACAGTTGTCTCTCCAGCAGTCTTCTCAGTGGT CTCTCCAGCTTCCTCAGCAGTCCTCCCAGCAGTCTCCTTAGAAGTCCCGTTGACGGCTTCAGTGACATCC CCAAAAGCCTCTCCCGTAACTTCCCCAGCAGCTGCCTTTCCAACAGCCTCCCCAGCAAATAAGGATGTCA GCAGCTTTCTAGAAACCACTGCTGACGTGGAAGAGATCACTGGAGAAGGACTCACTGCTTCTGGTAGTGG TGATGTCATGAGGAGACGTATTGCTACCCCAGAAGAAGTTCGTCTTCCCCTCCAACATGGGTGGCGGAGA GAGGTGCGCATCAAGAAGGGCAGCCACCGATGGCAGGGGGAGACCTGGTATTATGGCCCCTGTGGGAAGA GGATGAAGCAATTTCCAGAAGTGATCAAGTACCTGAGCCGCAACGTGGTACACAGTGTCCGCCGAGAGCA CTTCAGCTTCAGTCCCCGTATGCCTGTTGGAGATTTCTTTGAAGAAAGAGACACGCCAGAGGGCTTGCAG TGGGTGCAGCTCTCAGCAGAGGAGATCCCGTCGAGGATTCAGGCAATTACTGGCAAACGGGGTCGACCTC GAAACACTGAGAAGGCTAAGACTAAGGAAGTCCCCAAGGTGAAACGGGGTCGAGGTCGGCCACCTAAGGT CAAAATCACTGAGCTATTGAACAAGACAGACAACCGCCCCCTAAAGAAACTGGAGGCCCAAGAAACATTG AATGAGGAGGATAAAGCAAAGATTGCTAAAAGCAAGAAGAAGATGAGGCAGAAGGTTCAACGGGGAGAGT GTCAGACTACTATCCAAGGGCAGGCCAGAAATAAGCGGAAACAAGAGACCAAGAGCTTAAAGCAGAAGGA AGCTAAGAAGAAATCCAAGGCTGAGAAAGAAAAAGGAAAGACAAAGCAGGAAAAACTGAAGGAAAAAGTC AAGAGGGAAAAGAAGGAGAAGGTAAAAATGAAGGAAAAGGAGGAGGTGACCAAAGCCAAGCCAGCCTGTA AAGCAGATAAGACCCTGGCCACACAGAGGCGCTTGGAGGAACGGCAGAGGCAGCAGATGATCTTGGAGGA AATGAAGAAGCCGACAGAGGATATGTGTCTGACTGACCACCAGCCCCTGCCTGACTTCTCACGAGTCCCT GGTCTGACATTGCCCAGTGGAGCCTTCTCAGACTGCTTGACCATTGTGGAGTTCCTGCATAGCTTTGGCA AGGTGCTGGGCTTTGATCCTGCCAAAGATGTGCCTAGCCTGGGGGTCCTGCAGGAGGGACTCCTGTGTCA AGGTGACAGCTTGGGTGAGGTGCAAGACCTGCTGGTCAGGCTGCTGAAGGCTGCACTCCATGATCCTGGC TTTCCCTCCTACTGTCAGTCCCTAAAGATCTTGGGGGAGAAGGTGTCTGAGATCCCACTGACAAGAGACA ATGTGTCAGAGATCCTGCGCTGCTTCCTTATGGCATATGGAGTAGAGCCAGCCCTCTGTGACCGCCTGCG CACCCAGCCTTTTCAGGCCCAGCCACCCCAGCAGAAGGCTGCTGTCCTGGCCTTCCTTGTGCATGAGCTC AATGGCTCCACCCTCATCATCAATGAGATTGACAAGACTCTGGAGAGTATGTCCAGCTACAGGAAAAACA AGTGGATTGTTGAAGGCCGGCTCCGGAGGCTGAAAACTGTTCTGGCCAAGCGAACTGGGCGGTCTGAAGT AGAGATGGAAGGGCCAGAGGAATGCCTGGGACGGAGGCGCAGTTCTCGGATCATGGAGGAGACCAGTGGC ATGGAAGAAGAGGAAGAAGAGGAGTCTATAGCAGCTGTCCCTGGCCGCAGGGGTCGAAGAGATGGAGAGG TTGATGCCACAGCATCTAGCATCCCAGAGCTAGAGCGCCAGATAGAAAAACTCAGCAAGCGTCAGCTTTT CTTTCGCAAAAAGCTGCTTCACTCATCCCAGATGCTTCGGGCGGTCTCCCTGGGTCAGGACCGCTACAGA CGTCGCTACTGGGTATTGCCGTATTTGGCTGGTATCTTTGTAGAAGGAACAGAGGGGAACTTAGTTCCTG AGGAGGTGATAAAGAAGGAAACTGACTCCTTAAAAGTGGCAGCCCATGCGTCACTCAACCCTGCCCTCTT CTCTATGAAGATGGAGTTAGCTGGCTCCAACACCACTGCCAGTTCTCCTGCCCGGGCCCGAGGCCGACCT CGAAAAACTAAGCCCGGGTCTATGCAACCTAGGCATCTTAAGTCCCCTGTCAGGGGTCAGGATTCAGAAC AGCCCCAGGCCCAGCTTCAGCCTGAGGCTCAGCTTCATGCTCCTGCCCAGCCCCAGCCTCAGCTTCAGCT TCAGCTTCAGTCCCATAAGGGGTTCCTGGAGCAAGAAGGCTCCCCTTTGTCACTGGGTCAGAGCCAGCAT GACCTCAGCCAGTCAGCCTTCCTGTCTTGGCTGAGCCAGACTCAGAGCCATAGCTCCCTGTTGAGCAGCT CAGTCCTCACACCTGATAGCAGTCCGGGAAAACTAGACCCAGCTCCATCACAACCCCCGGAGGAGCCAGA GCCTGATGAGGCAGAATCCAGCCCTGATCCTCAAGCACTCTGGTTTAACATCTCAGCCCAGATGCCCTGC AATGCTGCCCCCACACCGCCCCCTGCAGTTTCTGAGGACCAACCCACTCCCTCCCCTCAGCAGCTTGCCT CCTCCAAGCCAATGAATAGACCTAGTGCTGCCAACCCTTGTTCTCCAGTGCAGTTCTCTTCCACGCCCTT GGCTGGGTTGGCCCCTAAGAGGCGAGCAGGAGACCCTGGAGAAATGCCACAGAGTCCCACAGGGCTGGGA CAGCCCAAACGGAGAGGGAGACCTCCCAGTAAGTTCTTCAAACAGATGGAACAGCGTTACCTAACCCAGC TGACAGCCCAGCCTGTCCCACCTGAGATGTGCTCAGGCTGGTGGTGGATACGAGATCCTGAGATGTTGGA TGCCATGCTCAAGGCCCTACACCCCCGAGGTATCCGGGAGAAGGCACTTCACAAACACCTTAACAAGCAC AGGGACTTCTTGCAGGAAGTCTGCCTGCGGCCCTCAGCTGACCCCATCTTTGAGCCCAGGCAACTACCTG CCTTTCAAGAAGGGATTATGAGCTGGTCCCCCAAAGAGAAGACATACGAGACAGACCTAGCAGTGCTTCA ATGGGTAGAGGAGCTGGAGCAGCGGGTTATCATGTCTGATCTGCAGATTCGGGGCTGGACATGTCCTAGC CCAGACTCTACCCGTGAAGACTTGGCCTACTGTGAGCACCTCTCCGACTCCCAGGAGGATATCACCTGGC GAGGTCGGGGCAGGGAGGGACTGGCACCTCAGCGTAAAACTACCAACCCTTTGGACCTGGCTGTGATGCG GCTGGCTGCCCTGGAACAGAATGTAGAACGGCGGTACCTGCGGGAGCCCCTCTGGCCAACTCATGAGGTT GTGCTGGAGAAGGCCCTGCTTAGCACACCTAATGGTGCCCCTGAGGGCACCACTACAGAGATATCATATG AGATCACCCCTCGCATTCGTGTCTGGCGCCAGACCCTCGAGCGGTGCCGGAGCGCAGCCCAGGTGTGCTT GTGCCTGGGCCAGCTGGAGAGGTCCATTGCCTGGGAGAAGTCTGTCAACAAAGTGACATGTCTAGTCTGC CGGAAGGGTGACAATGATGAGTTTCTTCTGCTTTGTGATGGGTGTGACCGTGGCTGCCACATTTACTGCC ATCGTCCCAAGATGGAGGCTGTCCCAGAAGGAGATTGGTTCTGTACTGTCTGTTTGGCTCAGCAGGTGGA GGGAGAATTCACTCAGAAGCCTGGTTTCCCAAAGCGTGGCCAGAAGCGGAAAAGTGGTTATTCGCTGAAC TTCTCAGAGGGTGATGGCCGCCGACGCCGGGTACTGTTGAGGGGCCGAGAAAGCCCAGCAGCAGGGCCTC GGTACTCGGAAGAAGGGCTCTCCCCCTCCAAGCGGCGGCGACTCTCTATGCGGAACCACCACAGTGATCT CACATTTTGCGAGATTATCCTGATGGAGATGGAGTCCCATGATGCAGCCTGGCCTTTCCTAGAGCCTGTG AACCCACGTTTGGTGAGTGGGTACCGGCGCATCATCAAAAATCCTATGGATTTTTCCACCATGCGGGAGC GGCTGCTCAGGGGAGGGTACACCAGCTCAGAGGAGTTTGCGGCTGATGCCCTCCTGGTATTTGACAACTG CCAGACTTTCAACGAGGATGACTCTGAAGTAGGCAAGGCTGGGCACATCATGCGCCGCTTCTTCGAGAGC CGCTGGGAGGAGTTTTATCAGGGAAAACAGGCCAATCTGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001300905
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300905.1, NP_001287834.1
RefSeq Size	8943 bp
RefSeq ORF	5712 bp
Locus ID	11176
Protein Families	Druggable Genome
Gene Summary	Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. In the complex, it plays a central role by being recruited to rDNA and by targeting chromatin modifying enzymes such as HDAC1, leading to repress RNA polymerase I transcription. Recruited to rDNA via its interaction with TTF1 and its ability to recognize and bind histone H4 acetylated on 'Lys-16' (H4K16ac), leading to deacetylation of H4K5ac, H4K8ac, H4K12ac but not H4K16ac. Specifically binds pRNAs, 150-250 nucleotide RNAs that are complementary in sequence to the rDNA promoter; pRNA-binding is required for heterochromatin formation and rDNA silencing (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) represents use of an alternate promoter, differs in the 5' UTR, and uses a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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