BAZ2A (NM_001300905) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	TIP5; WALp3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240221 representing NM_001300905 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCAAACGACCATTTTAACTTTACTGGCCTTCCCCCTGCACCTGCTGCCTCAGGACTGAAACCCT CTCCTTCCTCAGGGGAGGGCCTCTACACTAACGGGTCTCCCATGAACTTCCCCCAGCAAGGGAAAAGTTT GAATGGGGATGTGAATGTTAATGGCTTATCTACTGTATCTCACACTACTACTTCAGGGATTTTGAACTCT GCTCCCCACTCCTCCAGCACCTCACACCTCCATCACCCCAGCGTGGCCTACGACTGTCTCTGGAACTACT CACAGTACCCATCTGCCAATCCTGGCAGCAACCTCAAGGACCCACCCCTTCTCTCCCAGTTCTCGGGGGG ACAATACCCACTCAACGGCATCCTTGGGGGCAGCCGGCAACCTTCATCCCCAAGTCATAACACTAACCTT CGGGCTGGGAGCCAAGAGTTCTGGGCCAACGGTACCCAGAGTCCCATGGGGCTTAACTTTGATTCACAAG AACTGTATGATTCCTTTCCTGACCAGAATTTTGAGGTGATGCCCAATGGACCCCCTAGTTTTTTCACCTC CCCACAGACTTCTCCTATGTTGGGATCTAGCATTCAAACCTTTGCACCCTCCCAGGAGGTAGGCAGTGGT ATCCATCCTGATGAGGCAGCAGAAAAGGAGATGACTTCAGTTGTGGCAGAGAATGGCACTGGCTTGGTAG GCAGCTTGGAGCTGGAAGAAGAGCAGCCAGAACTGAAGATGTGTGGCTACAATGGCTCTGTCCCTTCTGT GGAATCGTTACACCAAGAGGTCTCAGTCCTGGTCCCTGACCCCACAGTGAGCTGTTTAGATGATCCTTCA CATCTTCCTGATCAACTGGAAGACACTCCAATCCTCAGTGAAGACTCTCTGGAGCCCTTCAACTCTCTGG CACCAGAGCCAGTGAGTGGAGGACTATATGGTATTGATGACACGGAGCTGATGGGTGCAGAGGACAAGCT GCCTCTTGAGGACAGCCCTGTGATTTCTGCCCTTGATTGCCCTTCCCTCAATAATGCTACTGCCTTCAGT CTCCTGGCAGATGATAGTCAAACATCAACCTCTATCTTTGCCAGTCCCACCTCTCCACCTGTCCTAGGGG AGTCTGTCCTGCAAGATAACAGCTTTGACCTGAATAATGGTAGTGACGCTGAACAGGAAGAAATGGAAAC TCAATCTTCAGACTTCCCACCATCCCTGACCCAGCCAGCTCCTGATCAGTCATCCACTATTCAGCTACAT CCAGCAACCTCACCAGCAGTCTCGCCAACAACCTCCCCAGCAGTCTCCCTAGTGGTTTCTCCAGCAGCCT CCCCAGAAATCTCTCCAGAAGTTTGTCCCGCAGCTTCTACAGTTGTCTCTCCAGCAGTCTTCTCAGTGGT CTCTCCAGCTTCCTCAGCAGTCCTCCCAGCAGTCTCCTTAGAAGTCCCGTTGACGGCTTCAGTGACATCC CCAAAAGCCTCTCCCGTAACTTCCCCAGCAGCTGCCTTTCCAACAGCCTCCCCAGCAAATAAGGATGTCA GCAGCTTTCTAGAAACCACTGCTGACGTGGAAGAGATCACTGGAGAAGGACTCACTGCTTCTGGTAGTGG TGATGTCATGAGGAGACGTATTGCTACCCCAGAAGAAGTTCGTCTTCCCCTCCAACATGGGTGGCGGAGA GAGGTGCGCATCAAGAAGGGCAGCCACCGATGGCAGGGGGAGACCTGGTATTATGGCCCCTGTGGGAAGA GGATGAAGCAATTTCCAGAAGTGATCAAGTACCTGAGCCGCAACGTGGTACACAGTGTCCGCCGAGAGCA CTTCAGCTTCAGTCCCCGTATGCCTGTTGGAGATTTCTTTGAAGAAAGAGACACGCCAGAGGGCTTGCAG TGGGTGCAGCTCTCAGCAGAGGAGATCCCGTCGAGGATTCAGGCAATTACTGGCAAACGGGGTCGACCTC GAAACACTGAGAAGGCTAAGACTAAGGAAGTCCCCAAGGTGAAACGGGGTCGAGGTCGGCCACCTAAGGT CAAAATCACTGAGCTATTGAACAAGACAGACAACCGCCCCCTAAAGAAACTGGAGGCCCAAGAAACATTG AATGAGGAGGATAAAGCAAAGATTGCTAAAAGCAAGAAGAAGATGAGGCAGAAGGTTCAACGGGGAGAGT GTCAGACTACTATCCAAGGGCAGGCCAGAAATAAGCGGAAACAAGAGACCAAGAGCTTAAAGCAGAAGGA AGCTAAGAAGAAATCCAAGGCTGAGAAAGAAAAAGGAAAGACAAAGCAGGAAAAACTGAAGGAAAAAGTC AAGAGGGAAAAGAAGGAGAAGGTAAAAATGAAGGAAAAGGAGGAGGTGACCAAAGCCAAGCCAGCCTGTA AAGCAGATAAGACCCTGGCCACACAGAGGCGCTTGGAGGAACGGCAGAGGCAGCAGATGATCTTGGAGGA AATGAAGAAGCCGACAGAGGATATGTGTCTGACTGACCACCAGCCCCTGCCTGACTTCTCACGAGTCCCT GGTCTGACATTGCCCAGTGGAGCCTTCTCAGACTGCTTGACCATTGTGGAGTTCCTGCATAGCTTTGGCA AGGTGCTGGGCTTTGATCCTGCCAAAGATGTGCCTAGCCTGGGGGTCCTGCAGGAGGGACTCCTGTGTCA AGGTGACAGCTTGGGTGAGGTGCAAGACCTGCTGGTCAGGCTGCTGAAGGCTGCACTCCATGATCCTGGC TTTCCCTCCTACTGTCAGTCCCTAAAGATCTTGGGGGAGAAGGTGTCTGAGATCCCACTGACAAGAGACA ATGTGTCAGAGATCCTGCGCTGCTTCCTTATGGCATATGGAGTAGAGCCAGCCCTCTGTGACCGCCTGCG CACCCAGCCTTTTCAGGCCCAGCCACCCCAGCAGAAGGCTGCTGTCCTGGCCTTCCTTGTGCATGAGCTC AATGGCTCCACCCTCATCATCAATGAGATTGACAAGACTCTGGAGAGTATGTCCAGCTACAGGAAAAACA AGTGGATTGTTGAAGGCCGGCTCCGGAGGCTGAAAACTGTTCTGGCCAAGCGAACTGGGCGGTCTGAAGT AGAGATGGAAGGGCCAGAGGAATGCCTGGGACGGAGGCGCAGTTCTCGGATCATGGAGGAGACCAGTGGC ATGGAAGAAGAGGAAGAAGAGGAGTCTATAGCAGCTGTCCCTGGCCGCAGGGGTCGAAGAGATGGAGAGG TTGATGCCACAGCATCTAGCATCCCAGAGCTAGAGCGCCAGATAGAAAAACTCAGCAAGCGTCAGCTTTT CTTTCGCAAAAAGCTGCTTCACTCATCCCAGATGCTTCGGGCGGTCTCCCTGGGTCAGGACCGCTACAGA CGTCGCTACTGGGTATTGCCGTATTTGGCTGGTATCTTTGTAGAAGGAACAGAGGGGAACTTAGTTCCTG AGGAGGTGATAAAGAAGGAAACTGACTCCTTAAAAGTGGCAGCCCATGCGTCACTCAACCCTGCCCTCTT CTCTATGAAGATGGAGTTAGCTGGCTCCAACACCACTGCCAGTTCTCCTGCCCGGGCCCGAGGCCGACCT CGAAAAACTAAGCCCGGGTCTATGCAACCTAGGCATCTTAAGTCCCCTGTCAGGGGTCAGGATTCAGAAC AGCCCCAGGCCCAGCTTCAGCCTGAGGCTCAGCTTCATGCTCCTGCCCAGCCCCAGCCTCAGCTTCAGCT TCAGCTTCAGTCCCATAAGGGGTTCCTGGAGCAAGAAGGCTCCCCTTTGTCACTGGGTCAGAGCCAGCAT GACCTCAGCCAGTCAGCCTTCCTGTCTTGGCTGAGCCAGACTCAGAGCCATAGCTCCCTGTTGAGCAGCT CAGTCCTCACACCTGATAGCAGTCCGGGAAAACTAGACCCAGCTCCATCACAACCCCCGGAGGAGCCAGA GCCTGATGAGGCAGAATCCAGCCCTGATCCTCAAGCACTCTGGTTTAACATCTCAGCCCAGATGCCCTGC AATGCTGCCCCCACACCGCCCCCTGCAGTTTCTGAGGACCAACCCACTCCCTCCCCTCAGCAGCTTGCCT CCTCCAAGCCAATGAATAGACCTAGTGCTGCCAACCCTTGTTCTCCAGTGCAGTTCTCTTCCACGCCCTT GGCTGGGTTGGCCCCTAAGAGGCGAGCAGGAGACCCTGGAGAAATGCCACAGAGTCCCACAGGGCTGGGA CAGCCCAAACGGAGAGGGAGACCTCCCAGTAAGTTCTTCAAACAGATGGAACAGCGTTACCTAACCCAGC TGACAGCCCAGCCTGTCCCACCTGAGATGTGCTCAGGCTGGTGGTGGATACGAGATCCTGAGATGTTGGA TGCCATGCTCAAGGCCCTACACCCCCGAGGTATCCGGGAGAAGGCACTTCACAAACACCTTAACAAGCAC AGGGACTTCTTGCAGGAAGTCTGCCTGCGGCCCTCAGCTGACCCCATCTTTGAGCCCAGGCAACTACCTG CCTTTCAAGAAGGGATTATGAGCTGGTCCCCCAAAGAGAAGACATACGAGACAGACCTAGCAGTGCTTCA ATGGGTAGAGGAGCTGGAGCAGCGGGTTATCATGTCTGATCTGCAGATTCGGGGCTGGACATGTCCTAGC CCAGACTCTACCCGTGAAGACTTGGCCTACTGTGAGCACCTCTCCGACTCCCAGGAGGATATCACCTGGC GAGGTCGGGGCAGGGAGGGACTGGCACCTCAGCGTAAAACTACCAACCCTTTGGACCTGGCTGTGATGCG GCTGGCTGCCCTGGAACAGAATGTAGAACGGCGGTACCTGCGGGAGCCCCTCTGGCCAACTCATGAGGTT GTGCTGGAGAAGGCCCTGCTTAGCACACCTAATGGTGCCCCTGAGGGCACCACTACAGAGATATCATATG AGATCACCCCTCGCATTCGTGTCTGGCGCCAGACCCTCGAGCGGTGCCGGAGCGCAGCCCAGGTGTGCTT GTGCCTGGGCCAGCTGGAGAGGTCCATTGCCTGGGAGAAGTCTGTCAACAAAGTGACATGTCTAGTCTGC CGGAAGGGTGACAATGATGAGTTTCTTCTGCTTTGTGATGGGTGTGACCGTGGCTGCCACATTTACTGCC ATCGTCCCAAGATGGAGGCTGTCCCAGAAGGAGATTGGTTCTGTACTGTCTGTTTGGCTCAGCAGGTGGA GGGAGAATTCACTCAGAAGCCTGGTTTCCCAAAGCGTGGCCAGAAGCGGAAAAGTGGTTATTCGCTGAAC TTCTCAGAGGGTGATGGCCGCCGACGCCGGGTACTGTTGAGGGGCCGAGAAAGCCCAGCAGCAGGGCCTC GGTACTCGGAAGAAGGGCTCTCCCCCTCCAAGCGGCGGCGACTCTCTATGCGGAACCACCACAGTGATCT CACATTTTGCGAGATTATCCTGATGGAGATGGAGTCCCATGATGCAGCCTGGCCTTTCCTAGAGCCTGTG AACCCACGTTTGGTGAGTGGGTACCGGCGCATCATCAAAAATCCTATGGATTTTTCCACCATGCGGGAGC GGCTGCTCAGGGGAGGGTACACCAGCTCAGAGGAGTTTGCGGCTGATGCCCTCCTGGTATTTGACAACTG CCAGACTTTCAACGAGGATGACTCTGAAGTAGGCAAGGCTGGGCACATCATGCGCCGCTTCTTCGAGAGC CGCTGGGAGGAGTTTTATCAGGGAAAACAGGCCAATCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240221 representing NM_001300905 Red=Cloning site Green=Tags(s) MEANDHFNFTGLPPAPAASGLKPSPSSGEGLYTNGSPMNFPQQGKSLNGDVNVNGLSTVSHTTTSGILNS APHSSSTSHLHHPSVAYDCLWNYSQYPSANPGSNLKDPPLLSQFSGGQYPLNGILGGSRQPSSPSHNTNL RAGSQEFWANGTQSPMGLNFDSQELYDSFPDQNFEVMPNGPPSFFTSPQTSPMLGSSIQTFAPSQEVGSG IHPDEAAEKEMTSVVAENGTGLVGSLELEEEQPELKMCGYNGSVPSVESLHQEVSVLVPDPTVSCLDDPS HLPDQLEDTPILSEDSLEPFNSLAPEPVSGGLYGIDDTELMGAEDKLPLEDSPVISALDCPSLNNATAFS LLADDSQTSTSIFASPTSPPVLGESVLQDNSFDLNNGSDAEQEEMETQSSDFPPSLTQPAPDQSSTIQLH PATSPAVSPTTSPAVSLVVSPAASPEISPEVCPAASTVVSPAVFSVVSPASSAVLPAVSLEVPLTASVTS PKASPVTSPAAAFPTASPANKDVSSFLETTADVEEITGEGLTASGSGDVMRRRIATPEEVRLPLQHGWRR EVRIKKGSHRWQGETWYYGPCGKRMKQFPEVIKYLSRNVVHSVRREHFSFSPRMPVGDFFEERDTPEGLQ WVQLSAEEIPSRIQAITGKRGRPRNTEKAKTKEVPKVKRGRGRPPKVKITELLNKTDNRPLKKLEAQETL NEEDKAKIAKSKKKMRQKVQRGECQTTIQGQARNKRKQETKSLKQKEAKKKSKAEKEKGKTKQEKLKEKV KREKKEKVKMKEKEEVTKAKPACKADKTLATQRRLEERQRQQMILEEMKKPTEDMCLTDHQPLPDFSRVP GLTLPSGAFSDCLTIVEFLHSFGKVLGFDPAKDVPSLGVLQEGLLCQGDSLGEVQDLLVRLLKAALHDPG FPSYCQSLKILGEKVSEIPLTRDNVSEILRCFLMAYGVEPALCDRLRTQPFQAQPPQQKAAVLAFLVHEL NGSTLIINEIDKTLESMSSYRKNKWIVEGRLRRLKTVLAKRTGRSEVEMEGPEECLGRRRSSRIMEETSG MEEEEEEESIAAVPGRRGRRDGEVDATASSIPELERQIEKLSKRQLFFRKKLLHSSQMLRAVSLGQDRYR RRYWVLPYLAGIFVEGTEGNLVPEEVIKKETDSLKVAAHASLNPALFSMKMELAGSNTTASSPARARGRP RKTKPGSMQPRHLKSPVRGQDSEQPQAQLQPEAQLHAPAQPQPQLQLQLQSHKGFLEQEGSPLSLGQSQH DLSQSAFLSWLSQTQSHSSLLSSSVLTPDSSPGKLDPAPSQPPEEPEPDEAESSPDPQALWFNISAQMPC NAAPTPPPAVSEDQPTPSPQQLASSKPMNRPSAANPCSPVQFSSTPLAGLAPKRRAGDPGEMPQSPTGLG QPKRRGRPPSKFFKQMEQRYLTQLTAQPVPPEMCSGWWWIRDPEMLDAMLKALHPRGIREKALHKHLNKH RDFLQEVCLRPSADPIFEPRQLPAFQEGIMSWSPKEKTYETDLAVLQWVEELEQRVIMSDLQIRGWTCPS PDSTREDLAYCEHLSDSQEDITWRGRGREGLAPQRKTTNPLDLAVMRLAALEQNVERRYLREPLWPTHEV VLEKALLSTPNGAPEGTTTEISYEITPRIRVWRQTLERCRSAAQVCLCLGQLERSIAWEKSVNKVTCLVC RKGDNDEFLLLCDGCDRGCHIYCHRPKMEAVPEGDWFCTVCLAQQVEGEFTQKPGFPKRGQKRKSGYSLN FSEGDGRRRRVLLRGRESPAAGPRYSEEGLSPSKRRRLSMRNHHSDLTFCEIILMEMESHDAAWPFLEPV NPRLVSGYRRIIKNPMDFSTMRERLLRGGYTSSEEFAADALLVFDNCQTFNEDDSEVGKAGHIMRRFFES RWEEFYQGKQANL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300905
ORF Size	5709 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300905.2
RefSeq Size	8943 bp
RefSeq ORF	5712 bp
Locus ID	11176
Protein Families	Druggable Genome
MW	211.4 kDa
Gene Summary	Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. In the complex, it plays a central role by being recruited to rDNA and by targeting chromatin modifying enzymes such as HDAC1, leading to repress RNA polymerase I transcription. Recruited to rDNA via its interaction with TTF1 and its ability to recognize and bind histone H4 acetylated on 'Lys-16' (H4K16ac), leading to deacetylation of H4K5ac, H4K8ac, H4K12ac but not H4K16ac. Specifically binds pRNAs, 150-250 nucleotide RNAs that are complementary in sequence to the rDNA promoter; pRNA-binding is required for heterochromatin formation and rDNA silencing (By similarity).[UniProtKB/Swiss-Prot Function]

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