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Rubicon (RUBCN) (NM_014687) Human Untagged Clone
CAT＃: SC316580
KIAA0226 (untagged)-Human KIAA0226 (KIAA0226), transcript variant 2
CN¥ 18,720.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KIAA0226; RUBICON; SCAR15 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC316580 representing NM_014687.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCGGCCGGAGGGCGCGGGAATGGAGCTCGGAGGCGGCGAGGAGCGCCTGCCTGAGGAGAGCAGGAGG GAGCACTGGCAGTTGCTGGGTAATTTGAAGACGACGGTGGAGGGTTTGGTATCAACCAACAGCCCCAAC GTCTGGTCTAAGTATGGTGGCTTGGAGCGGCTTTGCAGGGACATGCAGAGCATCCTCTATCACGGGCTT ATCCGTGACCAGGCGTGCCGCCGCCAGACGGATTACTGGCAGTTCGTGAAAGACATCCGGTGGCTCAGT CCCCACTCAGCCCTTCACGTGGAGAAGTTCATCAGCGTGCACGAGAACGACCAGAGCAGTGCTGATGGT GCCAGTGAACGTGCTGTTGCCGAGCTGTGGCTGCAGCACAGCCTGCAGTACCACTGCCTCTCAGCCCAG CTCCGGCCCCTGCTCGGGGATAGACAGTATATCAGAAAATTCTACACAGATGCTGCCTTCCTGCTAAGT GACGCTCATGTCACGGCCATGCTGCAGTGCCTGGAAGCAGTGGAACAGAACAACCCCCGCCTCCTGGCT CAGATCGATGCGTCCATGTTTGCCAGAAAGCACGAGAGCCCGCTCCTGGTGACAAAGAGCCAGAGCCTG ACAGCCCTGCCCAGTTCCACATACACCCCTCCAAACAGCTATGCTCAGCATTCCTACTTTGGGTCCTTC TCTAGCCTCCACCAATCCGTGCCCAACAATGGCTCAGAGAGAAGATCTACTTCCTTTCCACTCTCTGGC CCTCCCCGGAAACCTCAAGAAAGCAGAGGGCACGTCTCACCAGCAGAGGATCAAACCATCCAAGCCCCC CCAGTTTCAGTCTCTGCACTAGCCAGGGATTCCCCTTTGACCCCAAATGAAATGAGCTCCAGTACTCTG ACCAGCCCCATAGAGGCATCCTGGGTCAGCAGCCAGAATGATTCCCCAGGTGATGCCAGTGAGGGGCCT GAGTACCTGGCCATTGGCAACTTGGACCCCCGAGGCCGGACTGCCAGCTGTCAGAGTCACAGCAGCAAT GCCGAGAGCAGCAGTTCCAATTTGTTCTCCTCCAGCAGCTCCCAGAAGCCAGATTCTGCTGCCTCTTCC TTAGGGGACCAGGAAGGAGGTGGGGAGAGCCAGCTGTCCAGTGTCCTCCGCAGGTCCAGCTTCTCAGAG GGGCAGACACTCACTGTCACCAGTGGGGCAAAGAAAAGCCACATTCGCTCCCATTCGGATACCAGCATT GCCTCCAGGGGAGCTCCAGAATCCTGCAATGATAAGGCGAAGTTGAGAGGCCCTTTGCCCTACTCTGGT CAAAGCAGTGAAGTCAGCACACCCAGCTCTCTGTACATGGAATATGAAGGTGGTCGGTACCTGTGCTCA GGGGAAGGCATGTTCCGAAGACCATCAGAAGGACAGTCCCTCATCAGCTACCTCTCTGAGCAAGACTTC GGCAGCTGTGCCGACCTGGAAAAGGAGAATGCCCACTTCAGCATCTCAGAGTCCTTAATTGCTGCCATC GAGCTAATGAAGTGCAACATGATGAGCCAGTGCCTAGAGGAGGAGGAAGTGGAAGAGGAAGACAGTGAT AGAGAGATCCAGGAGCTGAAGCAGAAGATCCGCCTTCGGCGCCAGCAAATCCGCACCAAGAACCTGCTC CCCATGTACCAGGAGGCTGAGCACGGAAGCTTTCGGGTCACCTCCAGCAGCTCCCAGTTCAGCTCACGT GATTCGGCACAGCTCTCTGACTCTGGCTCTGCTGATGAGGTTGATGAATTTGAAATCCAAGATGCTGAC ATCAGAAGGAACACAGCCTCAAGCAGCAAATCCTTCGTTTCCTCCCAGTCCTTCTCCCACTGCTTCCTG CACTCCACGTCTGCTGAGGCGGTGGCCATGGGGCTCCTGAAGCAGTTTGAGGGGATGCAGCTTCCAGCC GCCTCGGAGCTGGAGTGGCTTGTCCCGGAGCATGATGCCCCTCAGAAGCTCCTGCCCATTCCTGACTCA CTGCCCATCTCACCGGATGACGGGCAGCACGCTGACATCTACAAGCTGCGGATTCGTGTTCGTGGCAAC TTGGAGTGGGCCCCGCCCCGGCCTCAGATAATTTTTAATGTTCATCCAGCCCCAACGAGGAAAATTGCC GTGGCCAAGCAGAATTACCGCTGTGCAGGATGTGGCATCCGGACTGACCCTGATTACATCAAGCGACTG CGGTACTGTGAGTACCTGGGCAAGTACTTCTGCCAGTGCTGCCACGAGAATGCCCAGATGGCCATCCCC AGCCGGGTTCTGCGCAAGTGGGACTTCAGCAAGTACTACGTCAGCAACTTCTCCAAGGACCTGCTCATT AAGATCTGGAATGATCCTCTCTTCAACGTGCAGGACATAAACAGTGCCCTCTATAGGAAGGTCAAGCTG CTCAATCAAGTCCGGCTGCTGCGGGTCCAGCTGTGTCACATGAAGAACATGTTCAAGACTTGCCGACTG GCCAAGGAGCTTCTGGATTCCTTTGACACAGTCCCAGGCCACCTGACAGAGGACCTCCACCTGTACTCA CTGAATGACCTGACTGCGACCAGGAAGGGGGAGCTGGGGCCCCGGCTTGCTGAGCTCACCAGGGCAGGG GCTACCCATGTGGAGAGATGCATGCTCTGCCAAGCCAAAGGCTTCATCTGTGAGTTCTGTCAGAATGAG GATGACATCATCTTTCCCTTTGAGCTCCATAAGTGCCGGACCTGTGAAGAGTGTAAAGCGTGTTACCAT AAAGCCTGCTTCAAGTCTGGAAGCTGTCCGCGCTGCGAGCGGCTGCAGGCCCGGCGGGAGGCACTGGCC AGGCAGAGCCTGGAGTCTTACCTGTCAGACTACGAGGAGGAGCCCGCGGAAGCGCTGGCCCTGGAAGCC GCCGTCCTGGAGGCCACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_014687 |
| Insert Size | 2919 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014687.2 |
| RefSeq Size | 6762 bp |
| RefSeq ORF | 2919 bp |
| Locus ID | 9711 |
| UniProt ID | Q92622 |
| MW | 108.6 kDa |
| Gene Summary | The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (2) represents a shorter transcript, but encodes the longer isoform (2). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220593 | KIAA0226 (Myc-DDK-tagged)-Human KIAA0226 (KIAA0226), transcript variant 2 |
CN¥ 10,680.00 |
|
| RC220593L3 | Lenti ORF clone of Human KIAA0226 (KIAA0226), transcript variant 2, Myc-DDK-tagged |
CN¥ 13,080.00 |
|
| RC220593L4 | Lenti ORF clone of Human KIAA0226 (KIAA0226), transcript variant 2, mGFP tagged |
CN¥ 9,030.00 |
|
| RG220593 | KIAA0226 (tGFP-tagged) - Human KIAA0226 (KIAA0226), transcript variant 2 |
CN¥ 7,890.00 |
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