United States
Germany
Japan
United Kingdom
China
Rubicon (RUBCN) (NM_014687) Human Tagged ORF Clone
CAT＃: RC220593
KIAA0226 (Myc-DDK-tagged)-Human KIAA0226 (KIAA0226), transcript variant 2
ORF Plasmid: tGFP
"NM_014687" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 10,680.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KIAA0226; RUBICON; SCAR15 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220593 representing NM_014687
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCCGGAGGGCGCGGGAATGGAGCTCGGAGGCGGCGAGGAGCGCCTGCCTGAGGAGAGCAGGAGGG AGCACTGGCAGTTGCTGGGTAATTTGAAGACGACGGTGGAGGGTTTGGTATCAACCAACAGCCCCAACGT CTGGTCTAAGTATGGTGGCTTGGAGCGGCTTTGCAGGGACATGCAGAGCATCCTCTATCACGGGCTTATC CGTGACCAGGCGTGCCGCCGCCAGACGGATTACTGGCAGTTCGTGAAAGACATCCGGTGGCTCAGTCCCC ACTCAGCCCTTCACGTGGAGAAGTTCATCAGCGTGCACGAGAACGACCAGAGCAGTGCTGATGGTGCCAG TGAACGTGCTGTTGCCGAGCTGTGGCTGCAGCACAGCCTGCAGTACCACTGCCTCTCAGCCCAGCTCCGG CCCCTGCTCGGGGATAGACAGTATATCAGAAAATTCTACACAGATGCTGCCTTCCTGCTAAGTGACGCTC ATGTCACGGCCATGCTGCAGTGCCTGGAAGCAGTGGAACAGAACAACCCCCGCCTCCTGGCTCAGATCGA TGCGTCCATGTTTGCCAGAAAGCACGAGAGCCCGCTCCTGGTGACAAAGAGCCAGAGCCTGACAGCCCTG CCCAGTTCCACATACACCCCTCCAAACAGCTATGCTCAGCATTCCTACTTTGGGTCCTTCTCTAGCCTCC ACCAATCCGTGCCCAACAATGGCTCAGAGAGAAGATCTACTTCCTTTCCACTCTCTGGCCCTCCCCGGAA ACCTCAAGAAAGCAGAGGGCACGTCTCACCAGCAGAGGATCAAACCATCCAAGCCCCCCCAGTTTCAGTC TCTGCACTAGCCAGGGATTCCCCTTTGACCCCAAATGAAATGAGCTCCAGTACTCTGACCAGCCCCATAG AGGCATCCTGGGTCAGCAGCCAGAATGATTCCCCAGGTGATGCCAGTGAGGGGCCTGAGTACCTGGCCAT TGGCAACTTGGACCCCCGAGGCCGGACTGCCAGCTGTCAGAGTCACAGCAGCAATGCCGAGAGCAGCAGT TCCAATTTGTTCTCCTCCAGCAGCTCCCAGAAGCCAGATTCTGCTGCCTCTTCCTTAGGGGACCAGGAAG GAGGTGGGGAGAGCCAGCTGTCCAGTGTCCTCCGCAGGTCCAGCTTCTCAGAGGGGCAGACACTCACTGT CACCAGTGGGGCAAAGAAAAGCCACATTCGCTCCCATTCGGATACCAGCATTGCCTCCAGGGGAGCTCCA GAATCCTGCAATGATAAGGCGAAGTTGAGAGGCCCTTTGCCCTACTCTGGTCAAAGCAGTGAAGTCAGCA CACCCAGCTCTCTGTACATGGAATATGAAGGTGGTCGGTACCTGTGCTCAGGGGAAGGCATGTTCCGAAG ACCATCAGAAGGACAGTCCCTCATCAGCTACCTCTCTGAGCAAGACTTCGGCAGCTGTGCCGACCTGGAA AAGGAGAATGCCCACTTCAGCATCTCAGAGTCCTTAATTGCTGCCATCGAGCTAATGAAGTGCAACATGA TGAGCCAGTGCCTAGAGGAGGAGGAAGTGGAAGAGGAAGACAGTGATAGAGAGATCCAGGAGCTGAAGCA GAAGATCCGCCTTCGGCGCCAGCAAATCCGCACCAAGAACCTGCTCCCCATGTACCAGGAGGCTGAGCAC GGAAGCTTTCGGGTCACCTCCAGCAGCTCCCAGTTCAGCTCACGTGATTCGGCACAGCTCTCTGACTCTG GCTCTGCTGATGAGGTTGATGAATTTGAAATCCAAGATGCTGACATCAGAAGGAACACAGCCTCAAGCAG CAAATCCTTCGTTTCCTCCCAGTCCTTCTCCCACTGCTTCCTGCACTCCACGTCTGCTGAGGCGGTGGCC ATGGGGCTCCTGAAGCAGTTTGAGGGGATGCAGCTTCCAGCCGCCTCGGAGCTGGAGTGGCTTGTCCCGG AGCATGATGCCCCTCAGAAGCTCCTGCCCATTCCTGACTCACTGCCCATCTCACCGGATGACGGGCAGCA CGCTGACATCTACAAGCTGCGGATTCGTGTTCGTGGCAACTTGGAGTGGGCCCCGCCCCGGCCTCAGATA ATTTTTAATGTTCATCCAGCCCCAACGAGGAAAATTGCCGTGGCCAAGCAGAATTACCGCTGTGCAGGAT GTGGCATCCGGACTGACCCTGATTACATCAAGCGACTGCGGTACTGTGAGTACCTGGGCAAGTACTTCTG CCAGTGCTGCCACGAGAATGCCCAGATGGCCATCCCCAGCCGGGTTCTGCGCAAGTGGGACTTCAGCAAG TACTACGTCAGCAACTTCTCCAAGGACCTGCTCATTAAGATCTGGAATGATCCTCTCTTCAACGTGCAGG ACATAAACAGTGCCCTCTATAGGAAGGTCAAGCTGCTCAATCAAGTCCGGCTGCTGCGGGTCCAGCTGTG TCACATGAAGAACATGTTCAAGACTTGCCGACTGGCCAAGGAGCTTCTGGATTCCTTTGACACAGTCCCA GGCCACCTGACAGAGGACCTCCACCTGTACTCACTGAATGACCTGACTGCGACCAGGAAGGGGGAGCTGG GGCCCCGGCTTGCTGAGCTCACCAGGGCAGGGGCTACCCATGTGGAGAGATGCATGCTCTGCCAAGCCAA AGGCTTCATCTGTGAGTTCTGTCAGAATGAGGATGACATCATCTTTCCCTTTGAGCTCCATAAGTGCCGG ACCTGTGAAGAGTGTAAAGCGTGTTACCATAAAGCCTGCTTCAAGTCTGGAAGCTGTCCGCGCTGCGAGC GGCTGCAGGCCCGGCGGGAGGCACTGGCCAGGCAGAGCCTGGAGTCTTACCTGTCAGACTACGAGGAGGA GCCCGCGGAAGCGCTGGCCCTGGAAGCCGCCGTCCTGGAGGCCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220593 representing NM_014687
Red=Cloning site Green=Tags(s) MRPEGAGMELGGGEERLPEESRREHWQLLGNLKTTVEGLVSTNSPNVWSKYGGLERLCRDMQSILYHGLI RDQACRRQTDYWQFVKDIRWLSPHSALHVEKFISVHENDQSSADGASERAVAELWLQHSLQYHCLSAQLR PLLGDRQYIRKFYTDAAFLLSDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTAL PSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSV SALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSS SNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAP ESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLE KENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEH GSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVA MGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQI IFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSK YYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVP GHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCR TCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014687 |
| ORF Size | 2916 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014687.4 |
| RefSeq Size | 6732 bp |
| RefSeq ORF | 2919 bp |
| Locus ID | 9711 |
| UniProt ID | Q92622 |
| MW | 108.4 kDa |
| Gene Summary | The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220593L3 | Lenti ORF clone of Human KIAA0226 (KIAA0226), transcript variant 2, Myc-DDK-tagged |
CNY 13,080.00 |
|
| RC220593L4 | Lenti ORF clone of Human KIAA0226 (KIAA0226), transcript variant 2, mGFP tagged |
CNY 9,030.00 |
|
| RG220593 | KIAA0226 (tGFP-tagged) - Human KIAA0226 (KIAA0226), transcript variant 2 |
CNY 7,890.00 |
|
| SC316580 | KIAA0226 (untagged)-Human KIAA0226 (KIAA0226), transcript variant 2 |
CNY 18,720.00 |
其它Rubicon产品
