Rubicon (RUBCN) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1 - 2 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon.
Formulation	PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	Rubicon antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 90, 101, 109 kDa; Observed: 91, 99 kDa
Gene Name	RUN and cysteine rich domain containing beclin 1 interacting protein
Database Link	XP_005269431 Entrez Gene 9711 Human UniProt ID Q92622
Background	Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).
Synonyms	ERp-72; ERP70; ERP72
Reference Data

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