RNF12 (RLIM) (NM_183353) Human Untagged Clone

CAT#: SC310213

RLIM (untagged)-Human ring finger protein, LIM domain interacting (RLIM), transcript variant 2



  "NM_183353" in other vectors (5)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
RLIM mouse monoclonal antibody,clone OTI2D7
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms MRX61; NY-REN-43; RNF12; TOKAS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC310213 representing NM_183353.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAAAACTCAGATTCCAATGACAAAGGAAGTGGTGATCAGTCTGCAGCACAGCGCAGAAGTCAGATG
GACCGATTGGATCGAGAAGAAGCTTTCTATCAATTTGTAAATAACCTGAGTGAAGAAGATTATAGGCTT
ATGAGAGATAACAATTTGCTAGGCACCCCAGGTGAAAGTACTGAGGAAGAGTTGCTGAGACGACTACAG
CAAATTAAAGAAGGCCCACCACCGCAAAACTCAGATGAAAATAGAGGAGGAGACTCTTCAGATGATGTG
TCTAATGGTGACTCTATAATAGACTGGCTTAACTCTGTCAGACAAACTGGAAATACAACAAGAAGTGGG
CAAAGAGGAAACCAATCTTGGAGAGCAGTGAGTCGGACTAATCCAAACAGTGGTGATTTCAGATTCAGT
TTAGAGATAAATGTTAACCGTAATAATGGGAGCCAAAATTCAGAGAATGAAAATGAGCCATCTGCAAGA
CGTTCTAGTGGAGAAAATGTGGAAAACAACAGCCAAAGGCAAGTGGAAAACCCACGATCTGAATCAACA
TCTGCAAGGCCATCTAGATCAGAACGAAATTCAACTGAAGCGTTAACAGAGGTCCCACCTACCAGAGGT
CAGAGGAGGGCAAGAAGCAGGAGCCCAGACCATCGGAGAACCAGAGCAAGAGCTGAAAGAAGTAGGTCA
CCTCTGCATCCAATGAGTGAAATTCCACGAAGATCTCATCATAGTATCTCATCTCAGACTTTTGAACAT
CCTTTGGTAAATGAGACGGAGGGAAGTTCTAGAACCCGGCACCATGTGACATTGAGGCAGCAAATATCT
GGGCCTGAGTTGCTAAGTAGAGGTCTTTTTGCAGCTTCTGGAACAAGAAATGCTTCTCAAGGAGCAGGT
TCTTCAGACACAGCTGCCAGTGGTGAATCTACAGGATCAGGACAGAGACCTCCAACCATAGTCCTTGAT
CTTCAAGTAAGAAGAGTTCGTCCTGGAGAATATCGGCAGAGAGATAGCATAGCCAGCAGAACTCGGTCT
AGGTCTCAGACACCAAACAACACTGTCACCTATGAAAGTGAACGAGGAGGTTTTAGGCGTACATTTTCA
CGTTCTGAGCGGGCAGGTGTGAGAACCTATGTCAGTACCATCAGAATTCCCATTCGTAGAATCTTAAAT
ACTGGTTTAAGTGAGACTACATCTGTTGCAATTCAGACCATGTTAAGGCAGATAATGACAGGTTTTGGT
GAGTTAAGCTATTTTATGTACAGTGATAGCGACTCAGAGCCTACTGGCTCAGTCTCAAATCGAAATATG
GAAAGGGCAGAGTCACGGAGTGGAAGAGGAGGTTCTGGTGGTGGTAGTAGTTCTGGTTCCAGTTCGAGT
TCCAGTTCCAGTTCGAGTTCCAGTTCCAGTTCAAGTTCCAGTTCCAGTCCTAGTTCCAGTTCCGGTGGT
GAAAGTTCAGAAACTAGCTCAGATTTATTTGAAGGCAGTAATGAAGGAAGCTCATCATCAGGCTCATCA
GGTGCCAGGCGAGAGGGTCGACATAGGGCCCCAGTCACATTTGATGAAAGTGGCTCTTTGCCCTTCCTT
AGCCTGGCTCAGTTTTTCCTCTTAAATGAGGATGATGATGACCAACCTAGAGGACTCACCAAAGAACAG
ATTGACAACTTGGCAATGAGAAGTTTTGGTGAAAATGATGCATTAAAAACCTGTAGTGTTTGCATTACA
GAATATACAGAAGGCAACAAACTTCGTAAACTACCTTGTTCCCATGAGTACCATGTCCACTGCATCGAT
CGCTGGTTATCTGAGAATTCTACCTGTCCTATTTGTCGCAGAGCAGTCTTAGCTTCTGGTAACAGAGAA
AGTGTTGTGTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_183353
Insert Size 1875 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_183353.2
RefSeq Size 10652 bp
RefSeq ORF 1875 bp
Locus ID 51132
UniProt ID Q9NVW2
Protein Families Druggable Genome, Transcription Factors
MW 68.5 kDa
Gene Summary The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009]
Transcript Variant: This variant (2) represents the longer transcript. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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