RNF12 (RLIM) (NM_183353) Human Untagged Clone
CAT#: SC320368
RLIM (untagged)-Human ring finger protein, LIM domain interacting (RLIM), transcript variant 2
CNY 4,656.00
CNY 7,220.00
Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | MRX61; NY-REN-43; RNF12; TOKAS |
Vector | pCMV6-AC |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>OriGene sequence for NM_183353.1
GGAAGATAATGGCTGCCTGAGCAACGTCTCCGAGCAGGCGCTGGGCTAGAGGCGGGTCTC
AACCAGCTACTCATTGGAGGCGGGCTTGAGAGCGGCGGCCAGGGAGGTGCGGAGCAGCCT CGGCGGCGGCGGCCGAACCAACCGAGTCGGATCCTGACCCTAAAACCTAGTATTTTCCAC TTGTTCATCAATATGGAAAACTCAGATTCCAATGACAAAGGAAGTGGTGATCAGTCTGCA GCACAGCGCAGAAGTCAGATGGACCGATTGGATCGAGAAGAAGCTTTCTATCAATTTGTA AATAACCTGAGTGAAGAAGATTATAGGCTTATGAGAGATAACAATTTGCTAGGCACCCCA GGTGAAAGTACTGAGGAAGAGTTGCTGAGACGACTACAGCAAATTAAAGAAGGCCCACCA CCGCAAAACTCAGATGAAAATAGAGGAGGAGACTCTTCAGATGATGTGTCTAATGGTGAC TCTATAATAGACTGGCTTAACTCTGTCAGACAAACTGGAAATACAACAAGAAGTGGGCAA AGAGGAAACCAATCTTGGAGAGCAGTGAGTCGGACTAATCCAAACAGTGGTGATTTCAGA TTCAGTTTAGAGATAAATGTTAACCGTAATAATGGGAGCCAAAATTCAGAGAATGAAAAT GAGCCATCTGCAAGACGTTCTAGTGGAGAAAATGTGGAAAACAACAGCCAAAGGCAAGTG GAAAACCCACGATCTGAATCAACATCTGCAAGGCCATCTAGATCAGAACGAAATTCAACT GAAGCGTTAACAGAGGTCCCACCTACCAGAGGTCAGAGGAGGGCAAGAAGCAGGAGCCCA GACCATCGGAGAACCAGAGCAAGAGCTGAAAGAAGTAGGTCACCTCTGCATCCAATGAGT GAAATTCCACGAAGATCTCATCATAGTATCTCATCTCAGACTTTTGAACATCCTTTGGTA AATGAGACGGAGGGAAGTTCTAGAACCCGGCACCATGTGACATTGAGGCAGCAAATATCT GGGCCTGAGTTGCTAAGTAGAGGTCTTTTTGCAGCTTCTGGAACAAGAAATGCTTCTCAA GGAGCAGGTTCTTCAGACACAGCTGCCAGTGGTGAATCTACAGGATCAGGACAGAGACCT CCAACCATAGTCCTTGATCTTCAAGTAAGAAGAGTTCGTCCTGGAGAATATCGGCAGAGA GATAGCATAGCCAGCAGAACTCGGTCTAGGTCTCAGACACCAAACAACACTGTCACCTAT GAAAGTGAACGAGGAGGTTTTAGGCGTACATTTTCACGTTCTGAGCGGGCAGGTGTGAGA ACCTATGTCAGTACCATCAGAATTCCCATTCGTAGAATCTTAAATACTGGTTTAAGTGAG ACTACATCTGTTGCAATTCAGACCATGTTAAGGCAGATAATGACAGGTTTTGGTGAGTTA AGCTATTTTATGTACAGTGATAGCGACTCAGAGCCTACTGGCTCAGTCTCAAATCGAAAT ATGGAAAGGGCAGAGTCACGGAGTGGAAGAGGAGGTTCTGGTGGTGGTAGTAGTTCTGGT TCCAGTTCGAGTTCCAGTTCCAGTTCGAGTTCCAGTTCCAGTTCAAGTTCCAGTTCCAGT CCTAGTTCCAGTTCCGGTGGTGAAAGTTCAGAAACTAGCTCAGATTTATTTGAAGGCAGT AATGAAGGAAGCTCATCATCAGGCTCATCAGGTGCCAGGCGAGAGGGTCGACATAGGGCC CCAGTCACATTTGATGAAAGTGGCTCTTTGCCCTTCCTTAGCCTGGCTCAGTTTTTCCTC TTAAATGAGGATGATGATGACCAACCTAGAGGACTCACCAAAGAACAGATTGACAACTTG GCAATGAGAAGTTTTGGTGAAAATGATGCATTAAAAACCTGTAGTGTTTGCATTACAGAA TATACAGAAGGCAACAAACTTCGTAAACTACCTTGTTCCCATGAGTACCATGTCCACTGC ATCGATCGCTGGTTATCTGAGAATTCTACCTGTCCTATTTGTCGCAGAGCAGTCTTAGCT TCTGGTAACAGAGAAAGTGTTGTGTAATTAAGATCTGAACTCTCAGCTATGTAGCTGATA TAGTGATGGGCAAACAGGAATCACTTGCTTTTATGTCCACTTTTTGAGTGGTACTTAAAT GTAAAGTAACAACCTGAATTGAGTCATTGCTTTCTGAAGGAATCATTGTCCTTTCTCCAG TTTTTGTTCCAGAATAAAAGGAAATATTTTAAAAGCCAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA |
Restriction Sites | Please inquire |
ACCN | NM_183353 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_183353.1, NP_899196.1 |
RefSeq Size | 3384 bp |
RefSeq ORF | 1875 bp |
Locus ID | 51132 |
UniProt ID | Q9NVW2 |
Protein Families | Druggable Genome, Transcription Factors |
Gene Summary | The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009] Transcript Variant: This variant (2) represents the longer transcript. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Single-stranded DNA-binding proteins regulate the abundance of LIM domain and LIM domain-binding proteins
,Z Xu, X Meng, Y Cai, H Liang, L Nagarajan, and SJ Brandt,
Genes Dev. 2007 Apr 15;21(8):942-55.
[RLIM]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203442 | RLIM (Myc-DDK-tagged)-Human ring finger protein, LIM domain interacting (RLIM), transcript variant 2 |
CNY 4,648.00 |
|
RC203442L3 | Lenti ORF clone of Human ring finger protein, LIM domain interacting (RLIM), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
RC203442L4 | Lenti ORF clone of Human ring finger protein, LIM domain interacting (RLIM), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
RG203442 | RLIM (tGFP-tagged) - Human ring finger protein, LIM domain interacting (RLIM), transcript variant 2 |
CNY 6,248.00 |
|
SC310213 | RLIM (untagged)-Human ring finger protein, LIM domain interacting (RLIM), transcript variant 2 |
CNY 7,220.00 |