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RNF12 (RLIM) (NM_183353) Human Tagged ORF Clone
CAT＃: RG203442
- TrueORF®
RLIM (tGFP-tagged) - Human ring finger protein, LIM domain interacting (RLIM), transcript variant 2
ORF Plasmid: DDK
"NM_183353" in other vectors (5)
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CNY 6,248.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MRX61; NY-REN-43; RNF12; TOKAS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203442 representing NM_183353
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAACTCAGATTCCAATGACAAAGGAAGTGGTGATCAGTCTGCAGCACAGCGCAGAAGTCAGATGG ACCGATTGGATCGAGAAGAAGCTTTCTATCAATTTGTAAATAACCTGAGTGAAGAAGATTATAGGCTTAT GAGAGATAACAATTTGCTAGGCACCCCAGGTGAAAGTACTGAGGAAGAGTTGCTGAGACGACTACAGCAA ATTAAAGAAGGCCCACCACCGCAAAACTCAGATGAAAATAGAGGAGGAGACTCTTCAGATGATGTGTCTA ATGGTGACTCTATAATAGACTGGCTTAACTCTGTCAGACAAACTGGAAATACAACAAGAAGTGGGCAAAG AGGAAACCAATCTTGGAGAGCAGTGAGTCGGACTAATCCAAACAGTGGTGATTTCAGATTCAGTTTAGAG ATAAATGTTAACCGTAATAATGGGAGCCAAAATTCAGAGAATGAAAATGAGCCATCTGCAAGACGTTCTA GTGGAGAAAATGTGGAAAACAACAGCCAAAGGCAAGTGGAAAACCCACGATCTGAATCAACATCTGCAAG GCCATCTAGATCAGAACGAAATTCAACTGAAGCGTTAACAGAGGTCCCACCTACCAGAGGTCAGAGGAGG GCAAGAAGCAGGAGCCCAGACCATCGGAGAACCAGAGCAAGAGCTGAAAGAAGTAGGTCACCTCTGCATC CAATGAGTGAAATTCCACGAAGATCTCATCATAGTATCTCATCTCAGACTTTTGAACATCCTTTGGTAAA TGAGACGGAGGGAAGTTCTAGAACCCGGCACCATGTGACATTGAGGCAGCAAATATCTGGGCCTGAGTTG CTAAGTAGAGGTCTTTTTGCAGCTTCTGGAACAAGAAATGCTTCTCAAGGAGCAGGTTCTTCAGACACAG CTGCCAGTGGTGAATCTACAGGATCAGGACAGAGACCTCCAACCATAGTCCTTGATCTTCAAGTAAGAAG AGTTCGTCCTGGAGAATATCGGCAGAGAGATAGCATAGCCAGCAGAACTCGGTCTAGGTCTCAGACACCA AACAACACTGTCACCTATGAAAGTGAACGAGGAGGTTTTAGGCGTACATTTTCACGTTCTGAGCGGGCAG GTGTGAGAACCTATGTCAGTACCATCAGAATTCCCATTCGTAGAATCTTAAATACTGGTTTAAGTGAGAC TACATCTGTTGCAATTCAGACCATGTTAAGGCAGATAATGACAGGTTTTGGTGAGTTAAGCTATTTTATG TACAGTGATAGCGACTCAGAGCCTACTGGCTCAGTCTCAAATCGAAATATGGAAAGGGCAGAGTCACGGA GTGGAAGAGGAGGTTCTGGTGGTGGTAGTAGTTCTGGTTCCAGTTCGAGTTCCAGTTCCAGTTCGAGTTC CAGTTCCAGTTCAAGTTCCAGTTCCAGTCCTAGTTCCAGTTCCGGTGGTGAAAGTTCAGAAACTAGCTCA GATTTATTTGAAGGCAGTAATGAAGGAAGCTCATCATCAGGCTCATCAGGTGCCAGGCGAGAGGGTCGAC ATAGGGCCCCAGTCACATTTGATGAAAGTGGCTCTTTGCCCTTCCTTAGCCTGGCTCAGTTTTTCCTCTT AAATGAGGATGATGATGACCAACCTAGAGGACTCACCAAAGAACAGATTGACAACTTGGCAATGAGAAGT TTTGGTGAAAATGATGCATTAAAAACCTGTAGTGTTTGCATTACAGAATATACAGAAGGCAACAAACTTC GTAAACTACCTTGTTCCCATGAGTACCATGTCCACTGCATCGATCGCTGGTTATCTGAGAATTCTACCTG TCCTATTTGTCGCAGAGCAGTCTTAGCTTCTGGTAACAGAGAAAGTGTTGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203442 representing NM_183353
Red=Cloning site Green=Tags(s) MENSDSNDKGSGDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQ IKEGPPPQNSDENRGGDSSDDVSNGDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLE INVNRNNGSQNSENENEPSARRSSGENVENNSQRQVENPRSESTSARPSRSERNSTEALTEVPPTRGQRR ARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPEL LSRGLFAASGTRNASQGAGSSDTAASGESTGSGQRPPTIVLDLQVRRVRPGEYRQRDSIASRTRSRSQTP NNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFM YSDSDSEPTGSVSNRNMERAESRSGRGGSGGGSSSGSSSSSSSSSSSSSSSSSSSSPSSSSGGESSETSS DLFEGSNEGSSSSGSSGARREGRHRAPVTFDESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLAMRS FGENDALKTCSVCITEYTEGNKLRKLPCSHEYHVHCIDRWLSENSTCPICRRAVLASGNRESVV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_183353 |
| ORF Size | 1872 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_183353.3 |
| RefSeq Size | 3384 bp |
| RefSeq ORF | 1875 bp |
| Locus ID | 51132 |
| UniProt ID | Q9NVW2 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203442 | RLIM (Myc-DDK-tagged)-Human ring finger protein, LIM domain interacting (RLIM), transcript variant 2 |
CNY 4,648.00 |
|
| RC203442L3 | Lenti ORF clone of Human ring finger protein, LIM domain interacting (RLIM), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC203442L4 | Lenti ORF clone of Human ring finger protein, LIM domain interacting (RLIM), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| SC310213 | RLIM (untagged)-Human ring finger protein, LIM domain interacting (RLIM), transcript variant 2 |
CNY 7,220.00 |
|
| SC320368 | RLIM (untagged)-Human ring finger protein, LIM domain interacting (RLIM), transcript variant 2 |
CNY 4,656.00 |
