UNC13B (NM_006377) Human Untagged Clone
CAT#: SC127356
UNC13B (untagged)-Human unc-13 homolog B (C. elegans) (UNC13B)
CNY 10,896.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | MUNC13; munc13-2; UNC13; Unc13h2 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC127356 sequence for NM_006377 edited (data generated by NextGen Sequencing)
ATGTCACTGCTCTGCGTGCGCGTTAAAAGGGCCAAATTCCAGGGTTCACCAGATAAATTT AACACATATGTGACCCTGAAAGTACAGAATGTGAAGAGCACAACTGTAGCAGTTCGTGGT GATCAGCCTTCCTGGGAACAGGATTTCATGTTTGAGATTAGTCGCCTGGACCTGGGTCTA AGTGTGGAGGTATGGAACAAAGGACTGATCTGGGACACCATGGTGGGGACTGTGTGGATT GCGCTGAAGACTATTCGTCAGTCGGATGAGGAAGGGCCTGGGGAATGGTCCACATTAGAG GCAGAGACGTTAATGAAAGACGATGAGATCTGTGGAACTAGAAACCCAACTCCTCATAAA ATTTTGCTTGATACAAGATTTGAGTTGCCTTTTGATATCCCAGAGGAGGAAGCCAGATAT TGGACCTACAAATGGGAGCAAATCAATGCCTTGGGAGCTGACAATGAGTATTCTAGTCAA GAAGAAAGCCAGAGGAAGCCATTGCCCACTGCTGCCGCCCAGTGTTCTTTTGAAGACCCT GATAGTGCCGTCGATGACCGAGATAGTGACTATCGCAGTGAGACCAGCAACAGCTTCCCA CCTCCTTACCATACAGCTTCCCAGCCCAACGCTTCTGTGCACCAGTTCCCTGTGCCGGTG CGATCGCCACAGCAGCTGCTACTTCAAGGCAGTTCCCGGGACTCTTGTAATGACTCTATG CAAAGTTATGACCTTGATTATCCAGAGCGGCGGGCTATCAGCCCCACCAGCAGCAGTAGG TATGGCTCCTCCTGTAATGTGAGTCAAGGAAGCTCTCAGCTAAGTGAACTAGACCAGTAT CACGAACAAGATGACGACCATCGGGAGACGGACTCGATTCATTCTTGCCACAGCTCTCAC AGCCTGTCCAGAGATGGCCAAGCAGGTTTTGGAGAACAAGAGAAACCCTTGGAGGTGACA GGTCAAGCAGAGAAGGAGGCAGCATGTGAACCCAAGGAGATGAAAGAAGATGCCACAACC CACCCTCCCCCAGATCTGGTGCTGCAAAAAGACCACTTCCTAGGTCCCCAGGAGAGTTTT CCTGAGGAGAATGCATCTTCACCATTTACCCAAGCCAGAGCACATTGGATCCGAGCAGTT ACCAAGGTTCGACTCCAGCTGCAGGAGATTCCAGATGATGGTGACCCCTCTCTGCCTCAG TGGCTCCCGGAAGGGCCAGCCGGAGGGCTCTATGGCATTGACAGCATGCCAGATTTACGC AGAAAGAAGCCACTGCCACTTGTCAGTGATCTGTCACTGGTCCAGTCTCGGAAGGCAGGA ATCACTTCTGCAATGGCTACACGCACTTCTCTTAAGGACGAAGAGCTGAAATCCCACGTG TATAAGAAAACCCTGCAGGCCTTAATCTACCCCATTTCGTGCACCACTCCTCATAACTTT GAGGTCTGGACGGCCACTACCCCAACCTACTGCTATGAGTGTGAAGGCCTGCTCTGGGGC ATTGCCCGGCAGGGCATGCGCTGCAGCGAATGTGGAGTCAAGTGCCATGAGAAGTGCCAG GATCTGCTCAATGCTGACTGCCTGCAGCGGGCTGCAGAAAAGAGCTGTAAACATGGAGCT GAGGACCGGACCCAGAACATTATCATGGCCATGAAGGACCGCATGAAGATCCGAGAGCGA AATAAGCCAGAGATCTTTGAAGTTATCCGGGACGTCTTCACAGTGAACAAAGCTGCCCAT GTGCAGCAGATGAAAACAGTGAAGCAGAGTGTACTGGATGGCACCTCCAAATGGTCAGCC AAGATCACCATTACTGTGGTGTGTGCCCAGGGCCTACAAGCCAAGGACAAAACAGGATCC AGTGACCCTTACGTGACTGTGCAAGTCAGCAAAACTAAGAAGCGTACCAAGACCATTTTT GGAAACTTGAATCCTGTTTGGGAGGAGAAGTTCCATTTTGAGTGCCACAACTCCTCTGAC CGCATTAAGGTGCGTGTATGGGATGAGGATGATGACATCAAGTCAAGAGTAAAGCAACGC CTAAAGCGAGAGTCTGATGATTTCCTTGGCCAAACCATCATTGAGGTTCGGACCCTAAGT GGCGAGATGGACGTCTGGTACAACTTGGAGAAGAGGACAGACAAATCAGCCGTCTCAGGG GCTATCCGACTACAAATCAGTGTGGAGATCAAGGGGGAGGAGAAAGTAGCCCCATACCAC GTGCAGTATACATGTCTCCATGAGAATCTTTTCCATTACCTCACAGACATTCAGGGCAGT GGAGGAGTCCGCATCCCTGAAGCTCGAGGAGACGATGCCTGGAAGGTGTACTTTGATGAG ACAGCCCAAGAAATTGTGGATGAATTTGCCATGCGTTATGGCATTGAGTCCATATATCAG GCCATGACGCACTTTGCATGTTTATCATCCAAGTACATGTGTCCTGGTGTGCCAGCAGTG ATGAGCACCTTACTGGCCAACATCAACGCCTACTATGCCCACACAACTGCCTCTACCAAT GTCTCTGCATCTGATCGCTTTGCAGCCTCCAACTTTGGGAAAGAGAGATTTGTAAAACTG CTGGACCAGCTACACAACTCACTGAGGATCGACCTCTCTACATACAGGAATAATTTCCCT GCTGGGAGTCCTGAACGGCTTCAGGACTTAAAATCCACAGTGGATTTGCTGACCAGCATT ACTTTCTTCAGAATGAAGGTACAAGAACTGCAAAGCCCTCCAAGAGCCAGCCAGGTGGTA AAGGATTGTGTGAAGGCCTGTTTGAACTCCACATATGAATATATCTTCAACAACTGCCAC GACTTATACAGCCGCCAGTACCAGCTGAAGCAGGAGCTACCTCCAGAGGAACAAGGGCCC AGCATTCGGAACCTGGATTTCTGGCCCAAGCTCATCACACTCATCGTGTCAATCATAGAG GAAGATAAGAATTCCTACACACCTGTTCTGAACCAGTTTCCTCAGGAGTTGAATGTGGGA AAAGTCAGCGCAGAAGTGATGTGGCATTTGTTTGCCCAAGACATGAAATATGCATTGGAG GAGCATGAGAAAGACCACCTGTGTAAAAGTGCTGACTACATGAACCTGCACTTCAAGGTG AAGTGGCTCCACAATGAATACGTGCGGGATCTGCCTGTCCTCCAGGGGCAGGTGCCTGAG TACCCAGCGTGGTTTGAGCAGTTCGTGCTACAATGGCTGGATGAGAATGAGGATGTATCC CTGGAATTCCTGCGTGGGGCCCTGGAACGAGATAAGAAGGATGGATTCCAGCAGACATCA GAGCATGCACTCTTTTCCTGCTCTGTGGTGGATGTCTTCACACAACTCAATCAGAGCTTT GAGATCATCCGGAAGCTGGAATGCCCAGACCCCAGCATCCTTGCCCACTACATGAGGAGG TTTGCTAAGACCATCGGGAAGGTGCTGATGCAGTATGCAGACATCTTGTCAAAGGACTTC CCAGCCTATTGCACAAAGGAGAAACTGCCCTGCATCCTGATGAACAACGTGCAGCAACTG AGGGTCCAGCTGGAGAAAATGTTTGAGGCCATGGGAGGCAAGGAGCTGGACCTTGAAGCT GCAGACAGTCTGAAGGAGCTGCAGGTGAAACTGAATACGGTTCTGGATGAGCTCAGCATG GTGTTTGGAAACAGTTTCCAGGTACGGATTGATGAGTGTGTTCGACAAATGGCCGACATC CTGGGCCAGGTTCGGGGCACAGGGAATGCATCTCCAGACGCCAGGGCCTCAGCGGCTCAG GATGCAGATAGCGTACTCCGGCCTCTCATGGACTTCCTGGATGGCAACCTCACCCTCTTT GCCACTGTGTGTGAGAAGACGGTTCTGAAGCGTGTACTGAAGGAGCTCTGGCGCGTGGTG ATGAACACAATGGAGAGGATGATTGTTCTGCCCCCACTCACTGACCAGACGGGCACCCAG CTGATCTTCACTGCTGCCAAGGAGCTGAGCCATCTTTCCAAACTCAAGGATCACATGGTA CGAGAGGAAACACGGAATCTCACTCCAAAGCAGTGTGCAGTCCTTGACCTCGCCCTGGAC ACCATCAAGCAATACTTCCATGCAGGAGGCAATGGGCTGAAGAAAACCTTCCTGGAGAAG AGCCCAGATCTGCAGTCTCTACGCTATGCCCTGTCTCTGTACACACAGACTACTGACACT CTCATCAAGACCTTTGTGCGCTCGCAGACCACCCAAGGGTCTGGTGTGGACGATCCTGTG GGAGAAGTCTCTATTCAGGTGGACTTGTTTACACACCCTGGTACTGGGGAGCACAAGGTC ACAGTGAAAGTGGTGGCTGCCAATGACCTCAAGTGGCAGACAGCGGGTATGTTCCGGCCT TTCGTGGAGGTGACTATGGTTGGCCCACACCAAAGTGATAAGAAGAGGAAGTTCACAACC AAATCCAAAAGCAACAACTGGGCCCCCAAGTACAATGAGACATTCCACTTCCTCCTGGGA AATGAGGAGGGGCCCGAGTCCTATGAGTTGCAGATATGCGTGAAGGATTACTGCTTTGCC CGGGAAGATCGCGTGCTAGGGCTGGCTGTGATGCCTCTGAGGGATGTCACAGCCAAGGGC AGCTGTGCCTGCTGGTGCCCCTTGGGCCGGAAGATCCATATGGATGAGACAGGCCTGACC ATTCTCCGGATTTTATCTCAGAGGAGCAATGACGAGGTGGCCCGAGAATTTGTGAAACTC AAATCAGAGTCTCGTTCCACGGAGGAGGGGAGCTGA Clone variation with respect to NM_006377.3 >OriGene 5' read for NM_006377 unedited
CGGCCGCGAATTCGGCACGAGCTTGCCCGATCCTCGGCCATGTCACTGCTCTGCGTGCGC GTTAAAAGGGCCAAATTCCAGGGTTCACCAGATAAATTTAACACATATGTGACCCTGAAA GTACAGAATGTGAAGAGCACAACTGTAGCAGTTCGTGGTGATCAGCCTTCCTGGGAACAG GATTTCATGTTTGAGATTAGTCGCCTGGACCGGGGTCTAAGTGTGGAGGTATGGAACAAA GGACTGATCTGGGACACCATGGTGGGGACTGTGTGGATTGCGCTGAAGACTATTCGTCAG TCGGATGAGGAAGGGCCTGGGGAATGGTCCACATTAGAGGCAGAGACGTTAATGAAAGAC GATGAGATCTGTGGAACTAGAAACCCAACTCCTCATAAAATTTTGCTTGATACAAGATTT GAGTTGCCTTTTGATATCCCAGAGGAGGAAGCCAGATATTGGACCTACAAATGGGAGCAA ATCAATGCCTTGGGAGCTGACAATGAGTATTCTAGTCAAGAAGAAAGCCAGAGGAAGCCA TTGCCCACTGCTGCCGCCCAGTGTTGTCACTGGACCTATTTGGGCTGGGGAGAACAACAG ACTTTTGAAGACCCTGATAGTGCCGTCGATGACCGAGATAGTGACTATCGCAGTGAGACC CAGCACAGCTTCCCACCTCCCTACCATACAGCTTCCCAGCCCAACGCTTNTGTGCACN >OriGene 3' read for NM_006377 unedited
TAGCTATGNAACGCGGCACGCAATCTAGTGATCGGTTTTTTTTTTTTTTTTTTTGGGTTC TTGTTTCATGTAATGGCCAGTACATCTGTGGGCAATGTCGAGTCCTCAGGAAGTCCAGGA GGCTGCTACAGAGGAAATCCAAGAACCATGTCACATCTCTCAACAAGTCTTGGGAAGTCC ATCTGACTCTCTGAAACAGTTTGTCTCTGACCTCCCAGGAAGTGTGGAGGGCCCCTTCCA TCCAGCCTGTACAGAGGGATCAGAGTCCAGGCTCCTTCTATAGGGTTGAATATCAGAGGG GAATAGCAAATGACCCCGATGAGAGAGAGAGAGACCAAAGGCTAGATTCTTTCTGCAAGG TGGAGGACGGCTAGAAGGCAGTGGCCACAGATGAGCAGGACTTTTTCTAGACTGGTTTCT ACAATTTTTGGCTTTGTTCAGGGCCCACCCAAGAAGAAAATACTCCTGGATCCTTCAACT AGCCCTCAGGTAGACACATGTTAGAACAGCCTGTCACGTCCTGAAGCATCACTCCCCTGC CTATAGGAGTGAGGCAGGGAGACTCCCATGCAGGTGGCCCCTAGTGCCTGGGGTCCAAAG AAATATTCTTCTCAAGGCTTTGAAGCACCACGTGAACCACATTCTACTCATGATACAATC TGGGGAAAGGGATTAGTACAGCCCATTTCCTAAAGGTATCCTGTTCAGTTTCAAAGCCCC TGCTCTCACTGGAGCCACAGAAAATCAGTGGATTCTACCCAAAGGACAGGCTAAATTCTT TTCTGTGGACTCCTGCCCTGGCCCTACTCCCATTCAGACCACCGGCTTAACACCAGGGCT AGCTGGCTTACCCAGCCGCCAGGCCCCAAGCCCCCACCTCTCAAACTTGGTCCTTCAATG GTGAGGTTGTATGCGTCCACATGAAAACGCGTTGCCAGCATATGGTCAAAGGGGGTACTA TTATTTCTCACCCTCCCTCTACA |
Restriction Sites | NotI-NotI |
ACCN | NM_006377 |
Insert Size | 6360 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006377.2, NP_006368.2 |
RefSeq Size | 6328 bp |
RefSeq ORF | 4776 bp |
Locus ID | 10497 |
UniProt ID | O14795 |
Domains | C2, DAG_PE-bind |
Protein Families | Druggable Genome |
Gene Summary | This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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UNC13B-A potential target gene of 9p13. 3 amplicon in prostate cancer
,VESAMÄKI, A;,
Thesis
[UNC13B]
|
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