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UNC13B (NM_006377) Human Tagged ORF Clone
CAT＃: RC219186
- TrueORF®
UNC13B (Myc-DDK-tagged)-Human unc-13 homolog B (C. elegans) (UNC13B)
ORF Plasmid: tGFP
"NM_006377" in other vectors (4)
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CNY 10,888.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MUNC13; munc13-2; UNC13; Unc13h2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219186 representing NM_006377
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGTCACTGCTCTGCGTGCGCGTTAAAAGGGCCAAATTCCAGGGTTCACCAGATAAATTTAACACATATG TGACCCTGAAAGTACAGAATGTGAAGAGCACAACTGTAGCAGTTCGTGGTGATCAGCCTTCCTGGGAACA GGATTTCATGTTTGAGATTAGTCGCCTGGACCTGGGTCTAAGTGTGGAGGTATGGAACAAAGGACTGATC TGGGACACCATGGTGGGGACTGTGTGGATTGCGCTGAAGACTATTCGTCAGTCGGATGAGGAAGGGCCTG GGGAATGGTCCACATTAGAGGCAGAGACGTTAATGAAAGACGATGAGATCTGTGGAACTAGAAACCCAAC TCCTCATAAAATTTTGCTTGATACAAGATTTGAGTTGCCTTTTGATATCCCAGAGGAGGAAGCCAGATAT TGGACCTACAAATGGGAGCAAATCAATGCCTTGGGAGCTGACAATGAGTATTCTAGTCAAGAAGAAAGCC AGAGGAAGCCATTGCCCACTGCTGCCGCCCAGTGTTCTTTTGAAGACCCTGATAGTGCCGTCGATGACCG AGATAGTGACTATCGCAGTGAGACCAGCAACAGCTTCCCACCTCCTTACCATACAGCTTCCCAGCCCAAC GCTTCTGTGCACCAGTTCCCTGTGCCGGTGCGATCGCCACAGCAGCTGCTACTTCAAGGCAGTTCCCGGG ACTCTTGTAATGACTCTATGCAAAGTTATGACCTTGATTATCCAGAGCGGCGGGCTATCAGCCCCACCAG CAGCAGTAGGTATGGCTCCTCCTGTAATGTGAGTCAAGGAAGCTCTCAGCTAAGTGAACTAGACCAGTAT CACGAACAAGATGACGACCATCGGGAGACGGACTCGATTCATTCTTGCCACAGCTCTCACAGCCTGTCCA GAGATGGCCAAGCAGGTTTTGGAGAACAAGAGAAACCCTTGGAGGTGACAGGTCAAGCAGAGAAGGAGGC AGCATGTGAACCCAAGGAGATGAAAGAAGATGCCACAACCCACCCTCCCCCAGATCTGGTGCTGCAAAAA GACCACTTCCTAGGTCCCCAGGAGAGTTTTCCTGAGGAGAATGCATCTTCACCATTTACCCAAGCCAGAG CACATTGGATCCGAGCAGTTACCAAGGTTCGACTCCAGCTGCAGGAGATTCCAGATGATGGTGACCCCTC TCTGCCTCAGTGGCTCCCGGAAGGGCCAGCCGGAGGGCTCTATGGCATTGACAGCATGCCAGATTTACGC AGAAAGAAGCCACTGCCACTTGTCAGTGATCTGTCACTGGTCCAGTCTCGGAAGGCAGGAATCACTTCTG CAATGGCTACACGCACTTCTCTTAAGGACGAAGAGCTGAAATCCCACGTGTATAAGAAAACCCTGCAGGC CTTAATCTACCCCATTTCGTGCACCACTCCTCATAACTTTGAGGTCTGGACGGCCACTACCCCAACCTAC TGCTATGAGTGTGAAGGCCTGCTCTGGGGCATTGCCCGGCAGGGCATGCGCTGCAGCGAATGTGGAGTCA AGTGCCATGAGAAGTGCCAGGATCTGCTCAATGCTGACTGCCTGCAGCGGGCTGCAGAAAAGAGCTGTAA ACATGGAGCTGAGGACCGGACCCAGAACATTATCATGGCCATGAAGGACCGCATGAAGATCCGAGAGCGA AATAAGCCAGAGATCTTTGAAGTTATCCGGGACGTCTTCACAGTGAACAAAGCTGCCCATGTGCAGCAGA TGAAAACAGTGAAGCAGAGTGTACTGGATGGCACCTCCAAATGGTCAGCCAAGATCACCATTACTGTGGT GTGTGCCCAGGGCCTACAAGCCAAGGACAAAACAGGATCCAGTGACCCTTACGTGACTGTGCAAGTCAGC AAAACTAAGAAGCGTACCAAGACCATTTTTGGAAACTTGAATCCTGTTTGGGAGGAGAAGTTCCATTTTG AGTGCCACAACTCCTCTGACCGCATTAAGGTGCGTGTATGGGATGAGGATGATGACATCAAGTCAAGAGT AAAGCAACGCCTAAAGCGAGAGTCTGATGATTTCCTTGGCCAAACCATCATTGAGGTTCGGACCCTAAGT GGCGAGATGGACGTCTGGTACAACTTGGAGAAGAGGACAGACAAATCAGCCGTCTCAGGGGCTATCCGAC TACAAATCAGTGTGGAGATCAAGGGGGAGGAGAAAGTAGCCCCATACCACGTGCAGTATACATGTCTCCA TGAGAATCTTTTCCATTACCTCACAGACATTCAGGGCAGTGGAGGAGTCCGCATCCCTGAAGCTCGAGGA GACGATGCCTGGAAGGTGTACTTTGATGAGACAGCCCAAGAAATTGTGGATGAATTTGCCATGCGTTATG GCATTGAGTCCATATATCAGGCCATGACGCACTTTGCATGTTTATCATCCAAGTACATGTGTCCTGGTGT GCCAGCAGTGATGAGCACCTTACTGGCCAACATCAACGCCTACTATGCCCACACAACTGCCTCTACCAAT GTCTCTGCATCTGATCGCTTTGCAGCCTCCAACTTTGGGAAAGAGAGATTTGTAAAACTGCTGGACCAGC TACACAACTCACTGAGGATCGACCTCTCTACATACAGGAATAATTTCCCTGCTGGGAGTCCTGAACGGCT TCAGGACTTAAAATCCACAGTGGATTTGCTGACCAGCATTACTTTCTTCAGAATGAAGGTACAAGAACTG CAAAGCCCTCCAAGAGCCAGCCAGGTGGTAAAGGATTGTGTGAAGGCCTGTTTGAACTCCACATATGAAT ATATCTTCAACAACTGCCACGACTTATACAGCCGCCAGTACCAGCTGAAGCAGGAGCTACCTCCAGAGGA ACAAGGGCCCAGCATTCGGAACCTGGATTTCTGGCCCAAGCTCATCACACTCATCGTGTCAATCATAGAG GAAGATAAGAATTCCTACACACCTGTTCTGAACCAGTTTCCTCAGGAGTTGAATGTGGGAAAAGTCAGCG CAGAAGTGATGTGGCATTTGTTTGCCCAAGACATGAAATATGCATTGGAGGAGCATGAGAAAGACCACCT GTGTAAAAGTGCTGACTACATGAACCTGCACTTCAAGGTGAAGTGGCTCCACAATGAATACGTGCGGGAT CTGCCTGTCCTCCAGGGGCAGGTGCCTGAGTACCCAGCGTGGTTTGAGCAGTTCGTGCTACAATGGCTGG ATGAGAATGAGGATGTATCCCTGGAATTCCTGCGTGGGGCCCTGGAACGAGATAAGAAGGATGGATTCCA GCAGACATCAGAGCATGCACTCTTTTCCTGCTCTGTGGTGGATGTCTTCACACAACTCAATCAGAGCTTT GAGATCATCCGGAAGCTGGAATGCCCAGACCCCAGCATCCTTGCCCACTACATGAGGAGGTTTGCTAAGA CCATCGGGAAGGTGCTGATGCAGTATGCAGACATCTTGTCAAAGGACTTCCCAGCCTATTGCACAAAGGA GAAACTGCCCTGCATCCTGATGAACAACGTGCAGCAACTGAGGGTCCAGCTGGAGAAAATGTTTGAGGCC ATGGGAGGCAAGGAGCTGGACCTTGAAGCTGCAGACAGTCTGAAGGAGCTGCAGGTGAAACTGAATACGG TTCTGGATGAGCTCAGCATGGTGTTTGGAAACAGTTTCCAGGTACGGATTGATGAGTGTGTTCGACAAAT GGCCGACATCCTGGGCCAGGTTCGGGGCACAGGGAATGCATCTCCAGACGCCAGGGCCTCAGCGGCTCAG GATGCAGATAGCGTACTCCGGCCTCTCATGGACTTCCTGGATGGCAACCTCACCCTCTTTGCCACTGTGT GTGAGAAGACGGTTCTGAAGCGTGTACTGAAGGAGCTCTGGCGCGTGGTGATGAACACAATGGAGAGGAT GATTGTTCTGCCCCCACTCACTGACCAGACGGGCACCCAGCTGATCTTCACTGCTGCCAAGGAGCTGAGC CATCTTTCCAAACTCAAGGATCACATGGTACGAGAGGAAACACGGAATCTCACTCCAAAGCAGTGTGCAG TCCTTGACCTCGCCCTGGACACCATCAAGCAATACTTCCATGCAGGAGGCAATGGGCTGAAGAAAACCTT CCTGGAGAAGAGCCCAGATCTGCAGTCTCTACGCTATGCCCTGTCTCTGTACACACAGACTACTGACACT CTCATCAAGACCTTTGTGCGCTCGCAGACCACCCAAGGGTCTGGTGTGGACGATCCTGTGGGAGAAGTCT CTATTCAGGTGGACTTGTTTACACACCCTGGTACTGGGGAGCACAAGGTCACAGTGAAAGTGGTGGCTGC CAATGACCTCAAGTGGCAGACAGCGGGTATGTTCCGGCCTTTCGTGGAGGTGACTATGGTTGGCCCACAC CAAAGTGATAAGAAGAGGAAGTTCACAACCAAATCCAAAAGCAACAACTGGGCCCCCAAGTACAATGAGA CATTCCACTTCCTCCTGGGAAATGAGGAGGGGCCCGAGTCCTATGAGTTGCAGATATGCGTGAAGGATTA CTGCTTTGCCCGGGAAGATCGCGTGCTAGGGCTGGCTGTGATGCCTCTGAGGGATGTCACAGCCAAGGGC AGCTGTGCCTGCTGGTGCCCCTTGGGCCGGAAGATCCATATGGATGAGACAGGCCTGACCATTCTCCGGA TTTTATCTCAGAGGAGCAATGACGAGGTGGCCCGAGAATTTGTGAAACTCAAATCAGAGTCTCGTTCCAC GGAGGAGGGGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219186 representing NM_006377
Red=Cloning site Green=Tags(s) MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLI WDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTRNPTPHKILLDTRFELPFDIPEEEARY WTYKWEQINALGADNEYSSQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSFPPPYHTASQPN ASVHQFPVPVRSPQQLLLQGSSRDSCNDSMQSYDLDYPERRAISPTSSSRYGSSCNVSQGSSQLSELDQY HEQDDDHRETDSIHSCHSSHSLSRDGQAGFGEQEKPLEVTGQAEKEAACEPKEMKEDATTHPPPDLVLQK DHFLGPQESFPEENASSPFTQARAHWIRAVTKVRLQLQEIPDDGDPSLPQWLPEGPAGGLYGIDSMPDLR RKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPHNFEVWTATTPTY CYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSCKHGAEDRTQNIIMAMKDRMKIRER NKPEIFEVIRDVFTVNKAAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVS KTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLS GEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVRIPEARG DDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTN VSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLTSITFFRMKVQEL QSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSRQYQLKQELPPEEQGPSIRNLDFWPKLITLIVSIIE EDKNSYTPVLNQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDHLCKSADYMNLHFKVKWLHNEYVRD LPVLQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSF EIIRKLECPDPSILAHYMRRFAKTIGKVLMQYADILSKDFPAYCTKEKLPCILMNNVQQLRVQLEKMFEA MGGKELDLEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPDARASAAQ DADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQTGTQLIFTAAKELS HLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDT LIKTFVRSQTTQGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPH QSDKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPESYELQICVKDYCFAREDRVLGLAVMPLRDVTAKG SCACWCPLGRKIHMDETGLTILRILSQRSNDEVAREFVKLKSESRSTEEGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006377 |
| ORF Size | 4773 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006377.5 |
| RefSeq Size | 6328 bp |
| RefSeq ORF | 4776 bp |
| Locus ID | 10497 |
| UniProt ID | O14795 |
| Domains | C2, DAG_PE-bind |
| Protein Families | Druggable Genome |
| MW | 180.5 kDa |
| Gene Summary | This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219186L3 | Lenti-ORF clone of UNC13B (Myc-DDK-tagged)-Human unc-13 homolog B (C. elegans) (UNC13B) |
CNY 13,288.00 |
|
| RC219186L4 | Lenti-ORF clone of UNC13B (mGFP-tagged)-Human unc-13 homolog B (C. elegans) (UNC13B) |
CNY 17,770.00 |
|
| RG219186 | UNC13B (tGFP-tagged) - Human unc-13 homolog B (C. elegans) (UNC13B) |
CNY 17,480.00 |
|
| SC127356 | UNC13B (untagged)-Human unc-13 homolog B (C. elegans) (UNC13B) |
CNY 10,896.00 |
