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MANEA (NM_024641) Human Untagged Clone
CAT＃: SC126732
MANEA (untagged)-Human mannosidase, endo-alpha (MANEA)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ENDO; hEndo |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC126732 sequence for NM_024641 edited (data generated by NextGen Sequencing)
ATGGCAAAGTTTCGGAGAAGGACTTGCATCATTTTGGCACTTTTTATTCTATTTATTTTC TCTCTGATGATGGGTTTAAAAATGCTGAGACCAAATACAGCTACTTTTGGAGCTCCTTTT GGACTTGACCTTCTTCCAGAACTTCATCAACGAACTATTCATTTGGGGAAAAATTTTGAT TTCCAAAAGAGTGACAGAATCAACAGTGAAACAAATACCAAGAATTTAAAAAGTGTTGAA ATCACTATGAAACCTTCCAAAGCCTCTGAACTTAACTTGGATGAACTACCACCTCTGAAC AATTATCTACATGTATTTTATTACAGTTGGTATGGAAATCCACAATTTGATGGTAAATAT ATACATTGGAATCATCCAGTGTTAGAGCATTGGGACCCTAGAATAGCCAAGAATTATCCA CAAGGGAGACACAACCCTCCAGATGACATTGGCTCCAGCTTTTATCCTGAATTGGGAAGT TACAGTTCTCGGGATCCTTCTGTCATAGAAACTCACATGAGACAAATGCGCTCAGCTTCA ATTGGTGTACTAGCCCTCTCTTGGTACCCACCTGATGTAAATGATGAAAATGGAGAACCT ACTGATAACTTGGTACCCACTATTTTGGATAAAGCTCATAAATATAACCTAAAGGTTACT TTTCACATAGAACCATATAGCAATCGAGATGATCAAAACATGTACAAAAATGTCAAGTAT ATTATAGACAAATATGGAAATCATCCGGCCTTTTACAGGTACAAGACGAAGACTGGCAAT GCTCTTCCTATGTTTTATGTCTATGATTCCTATATTACCAAGCCTGAAAAATGGGCCAAT CTGTTAACCACCTCAGGGTCTCGGAGTATTCGCAATTCTCCTTATGATGGACTGTTTATT GCCCTTCTGGTAGAAGAAAAACATAAGTATGATATTCTTCAAAGTGGTTTTGATGGAATT TACACATATTTTGCCACAAATGGCTTTACTTATGGCTCATCACATCAGAATTGGGCTAGC CTAAAATTATTTTGTGATAAATACAACTTAATATTTATCCCAAGTGTGGGCCCAGGATAC ATAGATACCAGCATCCGTCCATGGAACACGCAAAACACTCGGAACCGAATCAATGGGAAG TATTATGAAATTGGTCTGAGTGCCGCACTTCAGACACGCCCCAGCTTAATTTCTATCACC TCTTTTAATGAGTGGCATGAAGGAACTCAGATTGAAAAAGCTGTTCCCAAAAGAACCAGT AATACAGTGTACCTAGATTACCGTCCTCATAAACCAGGTCTTTACCTAGAACTGACTCGC AAGTGGTCTGAAAAATACAGTAAGGAAAGAGCAACTTATGCATTAGATCGCCAGCTGCCT GTTTCTTAA Clone variation with respect to NM_024641.3 >OriGene 5' read for NM_024641 unedited
TCACTATAGGCGGCCCGCGAATTCGCACGAGGGTTCGCGGTCTTAACCTCTCCTCTGGCC GAGTCCTTGCAAGAAGTGAATTACCCGACCCTGCAAAACACTTACTATTTTGGAGTTTAA AGTATGTCATCATGGCAAAGTTTCGGAGAAGGACTTGCATCATTTTGGCACTTTTTATTC TATTTATTTTCTCTCTGATGATGGGTTTAAAAATGCTGAGACCAAATACAGCTACTTTTG GAGCTCCTTTTGGACTTGACCTTCTTCCAGAACTTCATCAACGAACTATTCATTTGGGGA AAAATTTTGATTTCCAAAAGAGTGACAGAATCAACAGTGAAACAAATACCAAGAATTTAA AAAGTGTTGAAATCACTATGAAACCTTCCAAAGCCTCTGAACTTAACTTGGATGAACTAC CACCTCTGAACAATTATCTACATGTATTTTATTACAGTTGGTATGGAAATCCACAATTTG ATGGTAAATATATACATTGGAATCATCCAGTGTTAGAGCATTGGGACCCTAGAATAGCCA AGAATTATCCACAAGGGAGACACAACCCTCCAGATGACATTGGCTCCAGCTTTTATCCTG AATTGGGAAGTTACAGTTCTCGGGATCCTTCTGTCATAGAAACTCACATGAGACAAATGC GCTCAGCTTCAATTGGTGTACTAGCCCTCTCTTGGTACCCACCTGATGTAAATGATGAAA ATGGAGACCTACTGATAACTTGGTACCCACTATTNTGGATAAGCTCATAATATACCCTAA GTTACTTTTACATANAACATATAGCATCGAGATGATCAAACTGTCAAAATGTCAGTTTTT ATAGACAATATGAAATATCCGGCCTTC >OriGene 3' read for NM_024641 unedited
ATGGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT ATTTCATCTATGTTTTTATTAGGTAAAAATTAACCATTCCATATAACAAAATAGTAATTT TTAATTACAATCTTAAAACAGAAGGAAGTTAAACAACTCAAGGGAAGTTAATAACAGCAG TTTTTGACAATATTAGTCCTTAAAAGAAACAGTAAAATATTTGGTTTCTATAACAGTCAA AACAATTAGACTGATTAAACCATAAAAATTAATAACTTATAAAAGAATCCATTATGATTC ATGCATTGTATGTATTATACCACTTTAAGTTATACCATATTGAATTCAAACGTATAGTTT TGAATTAAGAAATAAATTCTTTGCTCTTATTTACTTGTTATCATAACAATTTAAACTGGG TTACAACTAAAATGGTAAAGGCAGTTGTAATAAATACCTGAGGAAGATTTTGAAAGTTTT CCTCTCTAAGAATTAACAGAATTATTAGAGTTATTTCCTAATGCAGTTGGCTCACCTTAT CTGTAGGTTCTACATCCATGGCCAAGAAATTGCCGACTGAAACTCTGAAGATGTACAGAC TCTTTTTTTTCATGTAATTATTCACTAACCAGTATGGTATAACAACTACATAGCATTTAC ATTGTATTAAGTGTTATAAATAATCTAGAGATGACTTAATGTATACAGGAGGATGTGCAT AGATTATATGCAACTACTATGCCAGTTTATATCAGGGACTTGAGCATCTGCAGATTTTGG TATCTATAGGGGTTACTGGNACCCATCCNCCACAGACACTGGGGATGACTGNAATTATTT TATTAAAAGGGGNAGAAAAACTATATAATTAAACTAGTACTATGAGATCGATGCCATNCT TCANGAGATAACTTTTGCATGAGCACGAACTGCTTACCTACTCGAAACTGTACTGCTGCT GTTAGGNTATGGGTGAGGACCCACAAACTAGAAGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_024641 |
| Insert Size | 4530 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024641.2, NP_078917.2 |
| RefSeq Size | 4593 bp |
| RefSeq ORF | 1389 bp |
| Locus ID | 79694 |
| UniProt ID | Q5SRI9 |
| Protein Families | Transmembrane |
| Gene Summary | N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM, Sep 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214726 | MANEA (Myc-DDK-tagged)-Human mannosidase, endo-alpha (MANEA) |
CNY 3,656.00 |
|
| RC214726L3 | Lenti ORF clone of Human mannosidase, endo-alpha (MANEA), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC214726L4 | Lenti ORF clone of Human mannosidase, endo-alpha (MANEA), mGFP tagged |
CNY 5,890.00 |
|
| RG214726 | MANEA (tGFP-tagged) - Human mannosidase, endo-alpha (MANEA) |
CNY 5,256.00 |
