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MANEA (NM_024641) Human Tagged ORF Clone
CAT＃: RC214726
MANEA (Myc-DDK-tagged)-Human mannosidase, endo-alpha (MANEA)
ORF Plasmid: tGFP
"NM_024641" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ENDO; hEndo |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214726 representing NM_024641
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAGTTTCGGAGAAGGACTTGCATCATTTTGGCACTTTTTATTCTATTTATTTTCTCTCTGATGA TGGGTTTAAAAATGCTGAGACCAAATACAGCTACTTTTGGAGCTCCTTTTGGACTTGACCTTCTTCCAGA ACTTCATCAACGAACTATTCATTTGGGGAAAAATTTTGATTTCCAAAAGAGTGACAGAATCAACAGTGAA ACAAATACCAAGAATTTAAAAAGTGTTGAAATCACTATGAAACCTTCCAAAGCCTCTGAACTTAACTTGG ATGAACTACCACCTCTGAACAATTATCTACATGTATTTTATTACAGTTGGTATGGAAATCCACAATTTGA TGGTAAATATATACATTGGAATCATCCAGTGTTAGAGCATTGGGACCCTAGAATAGCCAAGAATTATCCA CAAGGGAGACACAACCCTCCAGATGACATTGGCTCCAGCTTTTATCCTGAATTGGGAAGTTACAGTTCTC GGGATCCTTCTGTCATAGAAACTCACATGAGACAAATGCGCTCAGCTTCAATTGGTGTACTAGCCCTCTC TTGGTACCCACCTGATGTAAATGATGAAAATGGAGAACCTACTGATAACTTGGTACCCACTATTTTGGAT AAAGCTCATAAATATAACCTAAAGGTTACTTTTCACATAGAACCATATAGCAATCGAGATGATCAAAACA TGTACAAAAATGTCAAGTATATTATAGACAAATATGGAAATCATCCGGCCTTTTACAGGTACAAGACGAA GACTGGCAATGCTCTTCCTATGTTTTATGTCTATGATTCCTATATTACCAAGCCTGAAAAATGGGCCAAT CTGTTAACCACCTCAGGGTCTCGGAGTATTCGCAATTCTCCTTATGATGGACTGTTTATTGCCCTTCTGG TAGAAGAAAAACATAAGTATGATATTCTTCAAAGTGGTTTTGATGGAATTTACACATATTTTGCCACAAA TGGCTTTACTTATGGCTCATCACATCAGAATTGGGCTAGCCTAAAATTATTTTGTGATAAATACAACTTA ATATTTATCCCAAGTGTGGGCCCAGGATACATAGATACCAGCATCCGTCCATGGAACACGCAAAACACTC GGAACCGAATCAATGGGAAGTATTATGAAATTGGTCTGAGTGCCGCACTTCAGACACGCCCCAGCTTAAT TTCTATCACCTCTTTTAATGAGTGGCATGAAGGAACTCAGATTGAAAAAGCTGTTCCCAAAAGAACCAGT AATACAGTGTACCTAGATTACCGTCCTCATAAACCAGGTCTTTACCTAGAACTGACTCGCAAGTGGTCTG AAAAATACAGTAAGGAAAGAGCAACTTATGCATTAGATCGCCAGCTGCCTGTTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214726 representing NM_024641
Red=Cloning site Green=Tags(s) MAKFRRRTCIILALFILFIFSLMMGLKMLRPNTATFGAPFGLDLLPELHQRTIHLGKNFDFQKSDRINSE TNTKNLKSVEITMKPSKASELNLDELPPLNNYLHVFYYSWYGNPQFDGKYIHWNHPVLEHWDPRIAKNYP QGRHNPPDDIGSSFYPELGSYSSRDPSVIETHMRQMRSASIGVLALSWYPPDVNDENGEPTDNLVPTILD KAHKYNLKVTFHIEPYSNRDDQNMYKNVKYIIDKYGNHPAFYRYKTKTGNALPMFYVYDSYITKPEKWAN LLTTSGSRSIRNSPYDGLFIALLVEEKHKYDILQSGFDGIYTYFATNGFTYGSSHQNWASLKLFCDKYNL IFIPSVGPGYIDTSIRPWNTQNTRNRINGKYYEIGLSAALQTRPSLISITSFNEWHEGTQIEKAVPKRTS NTVYLDYRPHKPGLYLELTRKWSEKYSKERATYALDRQLPVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024641 |
| ORF Size | 1386 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024641.4 |
| RefSeq Size | 4593 bp |
| RefSeq ORF | 1389 bp |
| Locus ID | 79694 |
| UniProt ID | Q5SRI9 |
| Protein Families | Transmembrane |
| MW | 53.5 kDa |
| Gene Summary | N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM, Sep 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214726L3 | Lenti ORF clone of Human mannosidase, endo-alpha (MANEA), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC214726L4 | Lenti ORF clone of Human mannosidase, endo-alpha (MANEA), mGFP tagged |
CNY 5,890.00 |
|
| RG214726 | MANEA (tGFP-tagged) - Human mannosidase, endo-alpha (MANEA) |
CNY 5,256.00 |
|
| SC126732 | MANEA (untagged)-Human mannosidase, endo-alpha (MANEA) |
CNY 3,656.00 |
