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LDHA (NM_005566) Human Untagged Clone
CAT＃: SC116656
LDHA (untagged)-Human lactate dehydrogenase A (LDHA), transcript variant 1
CNY 3,600.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GSD11; HEL-S-133P; LDHM; PIG19 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116656 sequence for NM_005566 edited (data generated by NextGen Sequencing)
ATGGCAACTCTAAAGGATCAGCTGATTTATAATCTTCTAAAGGAAGAACAGACCCCCCAG AATAAGATTACAGTTGTTGGGGTTGGTGCTGTTGGCATGGCCTGTGCCATCAGTATCTTA ATGAAGGACTTGGCAGATGAACTTGCTCTTGTTGATGTCATCGAAGACAAATTGAAGGGA GAGATGATGGATCTCCAACATGGCAGCCTTTTCCTTAGAACACCAAAGATTGTCTCTGGC AAAGACTATAATGTAACTGCAAACTCCAAGCTGGTCATTATCACGGCTGGGGCACGTCAG CAAGAGGGAGAAAGCCGTCTTAATTTGGTCCAGCGTAACGTGAACATCTTTAAATTCATC ATTCCTAATGTTGTAAAATACAGCCCGAACTGCAAGTTGCTTATTGTTTCAAATCCAGTG GATATCTTGACCTACGTGGCTTGGAAGATAAGTGGTTTTCCCAAAAACCGTGTTATTGGA AGTGGTTGCAATCTGGATTCAGCCCGATTCCGTTACCTGATGGGGGAAAGGCTGGGAGTT CACCCATTAAGCTGTCATGGGTGGGTCCTTGGGGAACATGGAGATTCCAGTGTGCCTGTA TGGAGTGGAATGAATGTTGCTGGTGTCTCTCTGAAGACTCTGCACCCAGATTTAGGGACT GATAAAGATAAGGAACAGTGGAAAGAGGTTCACAAGCAGGTGGTTGAGAGTGCTTATGAG GTGATCAAACTCAAAGGCTACACATCCTGGGCTATTGGACTCTCTGTAGCAGATTTGGCA GAGAGTATAATGAAGAATCTTAGGCGGGTGCACCCAGTTTCCACCATGATTAAGGGTCTT TACGGAATAAAGGATGATGTCTTCCTTAGTGTTCCTTGCATTTTGGGACAGAATGGAATC TCAGACCTTGTGAAGGTGACTCTGACTTCTGAGGAAGAGGCCCGTTTGAAGAAGAGTGCA GATACACTTTGGGGGATCCAAAAGGAGCTGCAATTTTAA Clone variation with respect to NM_005566.3 483 c=>t;519 a=>g >OriGene 5' read for NM_005566 unedited
TACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTGCCGCCGATTCCGGATCTCA TTGCCACGCGCCCCCGACGACCGCCCGACGTGCATTCCCGATTCCTTTTGGTTCCAAGTC CAATATGGCAACTCTAAAGGATCAGCTGATTTATAATCTTCTAAAGGAAGAACAGACCCC CCAGAATAAGATTACAGTTGTTGGGGTTGGTGCTGTTGGCATGGCCTGTGCCATCAGTAT CTTAATGAAGGACTTGGCAGATGAACTTGCTCTTGTTGATGTCATCGAAGACAAATTGAA GGGAGAGATGATGGATCTCCAACATGGCAGCCTTTTCCTTAGAACACCAAAGATTGTCTC TGGCAAAGACTATAATGTAACTGCAAACTCCAAGCTGGTCATTATCACGGCTGGGGCACG TCAGCAAGAGGGAGAAAGCCGTCTTAATTTGGTCCAGCGTAACGTGAACATCTTTAAATT CATCATTCCTAATGTTGTAAAATACAGCCCGAACTGCAAGTTGCTTATTGTTTCAAATCC AGTGGATATCTTGACCTACGTGGCTTGGAAGATAAGTGGTTTTCCCAAAAACCGTGTTAT TGGAAAGTGGTTGCAATCTGGATTCAGCCCGATTCCGTTACCTGATGGGGGAAAGGGCTG GGAGTTCACCCATTAAGCTGTCATGGGGTGGGTCCTTGGGGAACATGGAGATTCCAGTGT GCCTGTATGGAGTGGAATGATGNTGCCTGGTGTCTCTCTGAGACTCTGCCCCAGATTANG GACTGATAAGATAAGGACAGTGNAAAAGAGTCACAAGCAGGTGGTTGAAAGTCTTATGGA GTGATCAACTCAAAGCTACCATNCTGGGCTATGGACTCTTGTAGCGATTTGCAGAAATTT ATGGAGATCTTAGGCGGTGCCCCAGTTCACATGAATAAAGGTCTTACGAAAGAGGAGAAG CTCCCCTAGGTCCTGCATTGGGACAATGAAT >OriGene 3' read for NM_005566 unedited
GCTATGGACCGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTAACTGTTAAAGGTTTATGGGGGGTTTTAGTTGGTATAACACTTGGATAGT GGGTTGCATTGTTTGTATGTAAATCTTTTTACATTATATGGAAAGGTCCACTACTGATAT AGTTCACAAAATAAAATCCTTTGGAAAAAATTATGCACAAAACATGATATTGGATTTATA CACTGGATCCCAGGATGGGACTCACTGGGAAAAAAATGTTGGACTAGGCATGTTCAGGGA AGGACCCAGGAAGTTATATAACACACGGTAAACATCCACCTGGCTCAAGGGGCAAATGCA GTACGTACAGCATTGGCAGGGGGGCGTCAAAGGGGGCAAAACTATTTCACACTACCCAGT TGAAGACTACCCAAGATTAATCCCTTCCAGCATCAGGATATAGCTGGGGATTTTACAAAC CATTCTTATTTCTAACTTCAGGAGTTGATGTTTTTCCCAGCCCATCTTAAAATATTACTG CTTTATTCACAGATCAGATAAAAAGGACAACTTGCCCAACCTCCACCTAAAATCCTGTTG TACCCTAAACAGGGAAATGATATGACATCAAAAGACTTTAAAATTGCAGCTCCTTTTGGA TCCCCCAAAGTGTATCTGCACTCTTCTTAAAACGGGCCTCTTCCTCAAAAGTCAGAGTCA CCTTCACAAGGTCTGAGATTCCATTCTGTCCCAAATGCAAGGACACTAAGGAAAACATCT CCTTTATTCCGTAAAACCCTTATCATGGTGGAAACTGGGTGCACCCGCCTAAGATTCTTA TTATACTCTCTGCCAAATCTCTACAGAGAGTCCATAGCCCAGAATGTGTAGCCTTGGAGT TGACACCTATAGCACTCTAACCACTGCTGGGACCCTTTTCATTGTTCTTACTTATAGCCC TAAATTGGNNGCAATCTN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005566 |
| Insert Size | 1690 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005566.1, NP_005557.1 |
| RefSeq Size | 1661 bp |
| RefSeq ORF | 999 bp |
| Locus ID | 3939 |
| UniProt ID | P00338 |
| Domains | ldh |
| Protein Families | Druggable Genome |
| Protein Pathways | Cysteine and methionine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism |
| Gene Summary | The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008] Transcript Variant: This variant (1) encodes the predominant isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209378 | LDHA (Myc-DDK-tagged)-Human lactate dehydrogenase A (LDHA), transcript variant 1 |
CNY 3,600.00 |
|
| RC209378L1 | Lenti ORF clone of Human lactate dehydrogenase A (LDHA), transcript variant 1, Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC209378L2 | Lenti ORF clone of Human lactate dehydrogenase A (LDHA), transcript variant 1, mGFP tagged |
CNY 6,000.00 |
|
| RC209378L3 | Lenti ORF clone of Human lactate dehydrogenase A (LDHA), transcript variant 1, Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC209378L4 | Lenti ORF clone of Human lactate dehydrogenase A (LDHA), transcript variant 1, mGFP tagged |
CNY 6,000.00 |
|
| RG209378 | LDHA (tGFP-tagged) - Human lactate dehydrogenase A (LDHA), transcript variant 1 |
CNY 5,200.00 |
|
| SC320978 | LDHA (untagged)-Human lactate dehydrogenase A (LDHA), transcript variant 1 |
CNY 3,600.00 |
