Sh3rf2 (NM_001034187) Rat Tagged Lenti ORF Clone
CAT#: RR210595L3
- LentiORF®
Lenti ORF clone of Sh3rf2 (Myc-DDK-tagged ORF) - Rat SH3 domain containing ring finger 2 (Sh3rf2), (10 ug)
"NM_001034187" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 11,210.00
CNY 600.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Posh2; Ppp1r39 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RR210595).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001034187 |
ORF Size | 2205 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001034187.1, NP_001029359.1 |
RefSeq Size | 2497 bp |
RefSeq ORF | 2208 bp |
Locus ID | 307472 |
UniProt ID | Q498M5 |
Gene Summary | Has E3 ubiquitin-protein ligase activity. Acts as an anti-apoptotic regulator of the JNK pathway by ubiquitinating and promoting the degradation of SH3RF1, a scaffold protein that is required for pro-apoptotic JNK activation (PubMed:22128169). Facilitates TNF-alpha-mediated recruitment of adapter proteins TRADD and RIPK1 to TNFRSF1A and regulates PAK4 protein stability via inhibition of its ubiquitin-mediated proteasomal degradation. Inhibits PPP1CA phosphatase activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN210595 | Sh3rf2 (untagged ORF) - Rat SH3 domain containing ring finger 2 (Sh3rf2), (10 ug) |
CNY 9,500.00 |
|
RR210595 | Sh3rf2 (Myc-DDK-tagged ORF) - Rat SH3 domain containing ring finger 2 (Sh3rf2), (10 ug) |
CNY 9,500.00 |
|
RR210595L4 | Lenti ORF clone of Sh3rf2 (mGFP-tagged ORF) - Rat SH3 domain containing ring finger 2 (Sh3rf2), (10 ug) |
CNY 12,350.00 |