Med1 (NM_001134361) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1559552
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209792 representing NM_001134361 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCTCTCTTGGACCGGCTCCATGCAAAATTTAACCAAAGTAGACCTTGGAGTGAGACCATTAAGC TTGTGCGTCAAGTCATGGAGAAGAGGGTCGTAATGAGTTCTGGAGGACATCAGCATTTGGTCAGCTGTTT GGAGACATTACAGAAGGCTCTCAAAGTAACATCTTTGCCAGCAATGACTGATCGTTTGGAATCCATAGCC AGACAGAATGGACTGGGCTCTCATCTCAGTGCCAGTGGCACTGAATGTTACATCACGTCAGATATGTTCT ATGTGGAAGTGCAGTTAGACCCTGCAGGACAGCTTTGTGATGTAAAAGTGGCTCACCATGGGGAGAATCC TGTGAGCTGTCCAGAGCTTGTGCAGCAGTTAAGGGAAAAGAATTTTGATGAGTTTTCTAAGCATCTTAAG GGTCTTGTTAATCTGTATAACCTTCCAGGAGACAACAAACTGAAGACTAAGATGTATCTGGCTCTCCAGT CCTTAGAACAGGACCTTTCTAAAATGGCAATTATGTACTGGAAGGCAACTAATGCTACTCCCTTGGATAA GATTCTTCACGGAAGTGTTGGCTATCTCACTCCACGGAGCGGGGGTCATTTAATGAACATGAAATACTAT GCCTCTCCGTCTGACCTGCTGCATGATACAACTGCCTCTCCTATCACTTTGCATGAAAAGAATGTTCCTC GGTCTTTGGGAATGAATGCCTCAGTGACAATTGAAGGAACCTCTGCTATGTACAAACTCCCAATTGCCCC ATTAATTATGGGGTCACACCCAGCTGACAACAAATGGACCCCTTCTTTCTCTGCAGTCACTAGTGCCAAC AGTGTTGATCTTCCTGCGTGTTTCTTCTTGAAATTTCCCCAGCCAATCCCAGTATCTAAAGCATTTGTTC AGAAACTGCAAAATTGCACGGGAATCCCGTTGTTTGAGACTCCGCCTACTTACCTGCCCCTCTATGAACT CATCACTCAGTTTGAGCTGTCAAAGGATCCTGACCCTTTACCTTTGAATCACAACATGCGATTTTACGCT GCTCTTCCAGGTCAGCAGCACTGCTATTTTCTCAATAAAGATGCTCCTCTTCCTGATGGCCAAAGCCTGC AGGGAACACTGGTCAGCAAAATCACCTTCCAGCACCCTGGCCGAGTTCCTCTTATCTTGAATATGATCAG ACACCAAGTGGCCTATAACACCCTAATTGGAAGCTGTGTTAAAAGAACTATTTTAAAAGAAGATTCTCCT GGGCTCCTCCAATTTGAAGTGTGTCCTCTTTCAGAATCTCGATTCAGTGTATCTTTTCAGCACCCTGTGA ATGACTCCCTTGTGTGTGTGGTGATGGACGTGCAAGACTCAACACATGTGAGCTGTAAACTCTACAAGGG GCTATCAGATGCACTGATCTGTACAGACGACTTCATTGCCAAAGTTGTTCAAAGATGTATGTCCATCCCT GTGACGATGAGGGCTATTCGGAGGAAGGCTGAAACCATACAGGCTGACACGCCAGCACTGTCCCTCATTG CAGAGACAGTCGAAGACATGGTGAAAAAGAACCTGCCCCCGGCTAGCAGCCCAGGGTATGGCATGACCAC AGGCAGCAACCCAATGAGCGGTACCACCACACCAACCAACACCTTTCCGGGGGGTCCCATTACCACCTTG TTTAATATGAGCATGAGCATCAAAGATCGGCATGAGTCGGTGGGCCATGGGGAGGACTTCAGCAAGGTGT CTCAGAACCCAATTCTTACCAGTTTGTTGCAAATCACAGGGAACGGGGGGTCTACCATTGGCTCGAGTCC GACCCCTCCTCATCACACGCCGCCACCTGTCTCTTCGATGGCCGGCAACACCAAGAACCACCCGATGCTC ATGAACCTTCTTAAAGATAACCCTGCCCAAGATTTCTCAACCCTTTATGGAAGCAGCCCTTTAGAAAGGC AGAACTCCTCTTCCGGATCACCCCGGATGGAAATGTGCTCGGGGAGCAACAAGACCAAGAAAAATAAGTC ATCAAGAGTACCACCGGACAAACCCAAGCACCAGACCGAAGATGATTTTCAGAGGGAGCTCTTTTCAATG GATGTCGACTCACAGAACCCTATGTTCGATGTCAGCATGACAGCTGACGCGCTGGATACACCTCATATCA CTCCAGCTCCAAGCCAGTGTAGTACGCCCCCAACAACGTACCCACAGCCAGTGTCTCACCCCCAGCCCAG TATTCAAAGGATGGGCCGACTGTCCAGTTCAGACAGCATTGGCCCAGATGTAACTGATATTCTTTCAGAC ATTGCAGAAGAAGCTTCAAAGCTTCCCAGCACTAGTGATGATTGCCCACCCATTGGTACCCCAGTTCGAG ATTCCTCGAGTTCTGGGCATTCTCAGAGTGCCCTCTTTGACTCTGATGTCTTTCAGACTAATAACAATGA AAATCCATACACTGACCCGGCTGACCTTATTGCAGATGCTGCTGGGAGCCCCAGTAGTGATTCTCCTACC AATCATTTTTTCCCTGATGGAGTAGATTTCAATCCTGATTTGTTGAACAGCCAAAGCCAAAGTGGTTTTG GAGAGGAGTATTTTGATGAAAGTAGTCAGAGTGGGGATAATGATGATTTCAAAGGATTTGCATCTCAGGC ACTAAATACTTTGGGGATGCCAATGCTCGGAGGTGACAATGGGGAGTCGAAATTGAAGGGCAGCAGCCAA GCTGACACAGTTGACTTCAGTATTCTGTCAGTAGCTGGTAAGGCTTTGGGTGCAGCAGACCTTCTGGAGC ACCACAGTGGTAGTCAGAGTCCTCTGCTGACCACTGGAGAATTAGGGAAAGAAAAAACTCAAAAGAGGGT GAAGGAAGGCAACGGTATGGGCGCTAGCAGTGGGTCAGGCCCGGGGTCAGACAGCAAGCCAGGCAAGCGC AGCCGCACACCTTCCAATGATGGGAAGAGCAAAGATAAGCCTCCAAAGCGAAAGAAGGCAGACACGGAGG GAAAATCCCCATCTCACAGTTCTAATAGACCTTTCACCCCACCCACCAGTACAGGTGGGTCCAAATCCCC TGGCAGTTCAGGACGATGTCAGACGCCCCCTGGTGTCGCCACACCGCCCATTCCCAAGATCACCATTCAG ATTCCTAAAGGAACAGTGATGGTGGGCAAGCCTTCCTCGCACAGTCAGTACTCTAGCAGTGGTTCTGTGT CTTCCTCTGGCAGCAAAAGCCACCACAGTCATTCTTCCTCCTCCTCTTCCTTATCTTCTGCTTCCACCTC AGGCAAGATGAAAAGCAGTAAATCAGAAGGCTCCTCAAGTGCCAAGCTCAGTGGCAGTATGTATTCTAGC CAAGGGTCTTCTGGATCCAGCCAGTCCAAAAATTCATCTCAGACTGGGGGGAAGCCAGGCTCCTCTCCCA TTACCAAACATGGACTGAGTAGTGGGTCTAGCAGCACCAAGATGAAACCTCAAGGCAAGCCATCCTCACT TATGAATCCTTCTATAAGTAAACCAAACATATCCCCTTCCCATTCAAGGCCTCCTGGAGGCTCAGATAAG CTTGCCTCTCCAATGAAGCCTGTTCCCGGGACCCCCCCATCCTCTAAAGCCAAGTCCCCTATCAGTTCAG GTTCTGGTAGCTCTCATGTGTCAGGAACTAGTTCAAGCTCTGGTATGAAGTCTTCTTCAGGGTCAGGATC CTCAGGCTCATTGTCTCAAAAAACTCCCCCAGCATCTAATTCTTGTACACCATCTTCCTCCTCATTTTCC TCAAGTGGTTCTTCCATGTCATCCTCTCAGAATCAACATGGGAGTTCCAAAGGGAAATCTCCCAGTAGGA ATAAGAAGCCTTCTTTGACAGCTGTCATAGATAAATTGAAGCATGGGGTGGTTACCAGTGGGCCTGGGGG TGAGGATCCAATAGACAGTCAGATGGGTGCAAGCACAAATTCCTCTAACCATCCCATGTCCTCCAAACAT AACATGTCAGGAGGAGAATTCCAGAGCAAACGTGAGAAAAGCGATAAAGACAAATCAAAGGTTTCTACTT CTGGAGGATCAGTTGATTCCTCTAAGAAGACTTCAGAGTCAAAAAATGTGGGGAGCACGGGTGTGGCAAA AATTATTATCAGCAAGCACGATGGAGGCTCCCCGAGCATCAAAGCCAAAGTGACTCTACAGAAACCTGGA GATAGTGGTGGAGAAGGGCTCAGGCCACAGATCGCCTCTTCAAAAAACTATGGCTCTCCATTGATCAGTG GTTCTACTCCAAAGCATGAACGGGGTTCTCCCAGCCACAGTAAGTCGCCAGCATATACACCTCAGAATGT GGACAGTGAAAGTGAGTCAGGCTCCTCCATAGCAGAGAGATCCTACCAGAACAGCCCCAGCTCAGATGAT GGTGTCCGGCCACTCCCAGAATACAGCACAGAGAAGCATAAGAAGCACAAAAAGGAAAAGAAAAAAGTAA AAGATAAAGATAGAGACCGGGACAGAGACAAAGACAGAGACAAGAAAAAATCTCACAGCATCAAGCCAGA GAATTGGTCTAAATCCCCCATTTCTTCAGATCAGACTTCATCTATGACAAATAACCCTATCTTATCTGCA GACAGGCCTTCCAGGCTCAGCCCTGACTTTATGATTGGGGAGGAAGATGATGATCTTATGGATGTGGCTC TGATTGGCAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001134361
Insert Size	4704 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001134361.1, NP_001127833.1
RefSeq Size	5813 bp
RefSeq ORF	4704 bp
Locus ID	497991

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