Med1 (NM_001134361) Rat Tagged ORF Clone
CAT#: RR209792
- TrueORF®
Med1 (Myc-DDK-tagged ORF) - Rat mediator complex subunit 1 (Med1), (10 ug)
"NM_001134361" in other vectors (1)
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CNY 25,180.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | RGD1559552 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209792 representing NM_001134361
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCTCTCTTGGACCGGCTCCATGCAAAATTTAACCAAAGTAGACCTTGGAGTGAGACCATTAAGC TTGTGCGTCAAGTCATGGAGAAGAGGGTCGTAATGAGTTCTGGAGGACATCAGCATTTGGTCAGCTGTTT GGAGACATTACAGAAGGCTCTCAAAGTAACATCTTTGCCAGCAATGACTGATCGTTTGGAATCCATAGCC AGACAGAATGGACTGGGCTCTCATCTCAGTGCCAGTGGCACTGAATGTTACATCACGTCAGATATGTTCT ATGTGGAAGTGCAGTTAGACCCTGCAGGACAGCTTTGTGATGTAAAAGTGGCTCACCATGGGGAGAATCC TGTGAGCTGTCCAGAGCTTGTGCAGCAGTTAAGGGAAAAGAATTTTGATGAGTTTTCTAAGCATCTTAAG GGTCTTGTTAATCTGTATAACCTTCCAGGAGACAACAAACTGAAGACTAAGATGTATCTGGCTCTCCAGT CCTTAGAACAGGACCTTTCTAAAATGGCAATTATGTACTGGAAGGCAACTAATGCTACTCCCTTGGATAA GATTCTTCACGGAAGTGTTGGCTATCTCACTCCACGGAGCGGGGGTCATTTAATGAACATGAAATACTAT GCCTCTCCGTCTGACCTGCTGCATGATACAACTGCCTCTCCTATCACTTTGCATGAAAAGAATGTTCCTC GGTCTTTGGGAATGAATGCCTCAGTGACAATTGAAGGAACCTCTGCTATGTACAAACTCCCAATTGCCCC ATTAATTATGGGGTCACACCCAGCTGACAACAAATGGACCCCTTCTTTCTCTGCAGTCACTAGTGCCAAC AGTGTTGATCTTCCTGCGTGTTTCTTCTTGAAATTTCCCCAGCCAATCCCAGTATCTAAAGCATTTGTTC AGAAACTGCAAAATTGCACGGGAATCCCGTTGTTTGAGACTCCGCCTACTTACCTGCCCCTCTATGAACT CATCACTCAGTTTGAGCTGTCAAAGGATCCTGACCCTTTACCTTTGAATCACAACATGCGATTTTACGCT GCTCTTCCAGGTCAGCAGCACTGCTATTTTCTCAATAAAGATGCTCCTCTTCCTGATGGCCAAAGCCTGC AGGGAACACTGGTCAGCAAAATCACCTTCCAGCACCCTGGCCGAGTTCCTCTTATCTTGAATATGATCAG ACACCAAGTGGCCTATAACACCCTAATTGGAAGCTGTGTTAAAAGAACTATTTTAAAAGAAGATTCTCCT GGGCTCCTCCAATTTGAAGTGTGTCCTCTTTCAGAATCTCGATTCAGTGTATCTTTTCAGCACCCTGTGA ATGACTCCCTTGTGTGTGTGGTGATGGACGTGCAAGACTCAACACATGTGAGCTGTAAACTCTACAAGGG GCTATCAGATGCACTGATCTGTACAGACGACTTCATTGCCAAAGTTGTTCAAAGATGTATGTCCATCCCT GTGACGATGAGGGCTATTCGGAGGAAGGCTGAAACCATACAGGCTGACACGCCAGCACTGTCCCTCATTG CAGAGACAGTCGAAGACATGGTGAAAAAGAACCTGCCCCCGGCTAGCAGCCCAGGGTATGGCATGACCAC AGGCAGCAACCCAATGAGCGGTACCACCACACCAACCAACACCTTTCCGGGGGGTCCCATTACCACCTTG TTTAATATGAGCATGAGCATCAAAGATCGGCATGAGTCGGTGGGCCATGGGGAGGACTTCAGCAAGGTGT CTCAGAACCCAATTCTTACCAGTTTGTTGCAAATCACAGGGAACGGGGGGTCTACCATTGGCTCGAGTCC GACCCCTCCTCATCACACGCCGCCACCTGTCTCTTCGATGGCCGGCAACACCAAGAACCACCCGATGCTC ATGAACCTTCTTAAAGATAACCCTGCCCAAGATTTCTCAACCCTTTATGGAAGCAGCCCTTTAGAAAGGC AGAACTCCTCTTCCGGATCACCCCGGATGGAAATGTGCTCGGGGAGCAACAAGACCAAGAAAAATAAGTC ATCAAGAGTACCACCGGACAAACCCAAGCACCAGACCGAAGATGATTTTCAGAGGGAGCTCTTTTCAATG GATGTCGACTCACAGAACCCTATGTTCGATGTCAGCATGACAGCTGACGCGCTGGATACACCTCATATCA CTCCAGCTCCAAGCCAGTGTAGTACGCCCCCAACAACGTACCCACAGCCAGTGTCTCACCCCCAGCCCAG TATTCAAAGGATGGGCCGACTGTCCAGTTCAGACAGCATTGGCCCAGATGTAACTGATATTCTTTCAGAC ATTGCAGAAGAAGCTTCAAAGCTTCCCAGCACTAGTGATGATTGCCCACCCATTGGTACCCCAGTTCGAG ATTCCTCGAGTTCTGGGCATTCTCAGAGTGCCCTCTTTGACTCTGATGTCTTTCAGACTAATAACAATGA AAATCCATACACTGACCCGGCTGACCTTATTGCAGATGCTGCTGGGAGCCCCAGTAGTGATTCTCCTACC AATCATTTTTTCCCTGATGGAGTAGATTTCAATCCTGATTTGTTGAACAGCCAAAGCCAAAGTGGTTTTG GAGAGGAGTATTTTGATGAAAGTAGTCAGAGTGGGGATAATGATGATTTCAAAGGATTTGCATCTCAGGC ACTAAATACTTTGGGGATGCCAATGCTCGGAGGTGACAATGGGGAGTCGAAATTGAAGGGCAGCAGCCAA GCTGACACAGTTGACTTCAGTATTCTGTCAGTAGCTGGTAAGGCTTTGGGTGCAGCAGACCTTCTGGAGC ACCACAGTGGTAGTCAGAGTCCTCTGCTGACCACTGGAGAATTAGGGAAAGAAAAAACTCAAAAGAGGGT GAAGGAAGGCAACGGTATGGGCGCTAGCAGTGGGTCAGGCCCGGGGTCAGACAGCAAGCCAGGCAAGCGC AGCCGCACACCTTCCAATGATGGGAAGAGCAAAGATAAGCCTCCAAAGCGAAAGAAGGCAGACACGGAGG GAAAATCCCCATCTCACAGTTCTAATAGACCTTTCACCCCACCCACCAGTACAGGTGGGTCCAAATCCCC TGGCAGTTCAGGACGATGTCAGACGCCCCCTGGTGTCGCCACACCGCCCATTCCCAAGATCACCATTCAG ATTCCTAAAGGAACAGTGATGGTGGGCAAGCCTTCCTCGCACAGTCAGTACTCTAGCAGTGGTTCTGTGT CTTCCTCTGGCAGCAAAAGCCACCACAGTCATTCTTCCTCCTCCTCTTCCTTATCTTCTGCTTCCACCTC AGGCAAGATGAAAAGCAGTAAATCAGAAGGCTCCTCAAGTGCCAAGCTCAGTGGCAGTATGTATTCTAGC CAAGGGTCTTCTGGATCCAGCCAGTCCAAAAATTCATCTCAGACTGGGGGGAAGCCAGGCTCCTCTCCCA TTACCAAACATGGACTGAGTAGTGGGTCTAGCAGCACCAAGATGAAACCTCAAGGCAAGCCATCCTCACT TATGAATCCTTCTATAAGTAAACCAAACATATCCCCTTCCCATTCAAGGCCTCCTGGAGGCTCAGATAAG CTTGCCTCTCCAATGAAGCCTGTTCCCGGGACCCCCCCATCCTCTAAAGCCAAGTCCCCTATCAGTTCAG GTTCTGGTAGCTCTCATGTGTCAGGAACTAGTTCAAGCTCTGGTATGAAGTCTTCTTCAGGGTCAGGATC CTCAGGCTCATTGTCTCAAAAAACTCCCCCAGCATCTAATTCTTGTACACCATCTTCCTCCTCATTTTCC TCAAGTGGTTCTTCCATGTCATCCTCTCAGAATCAACATGGGAGTTCCAAAGGGAAATCTCCCAGTAGGA ATAAGAAGCCTTCTTTGACAGCTGTCATAGATAAATTGAAGCATGGGGTGGTTACCAGTGGGCCTGGGGG TGAGGATCCAATAGACAGTCAGATGGGTGCAAGCACAAATTCCTCTAACCATCCCATGTCCTCCAAACAT AACATGTCAGGAGGAGAATTCCAGAGCAAACGTGAGAAAAGCGATAAAGACAAATCAAAGGTTTCTACTT CTGGAGGATCAGTTGATTCCTCTAAGAAGACTTCAGAGTCAAAAAATGTGGGGAGCACGGGTGTGGCAAA AATTATTATCAGCAAGCACGATGGAGGCTCCCCGAGCATCAAAGCCAAAGTGACTCTACAGAAACCTGGA GATAGTGGTGGAGAAGGGCTCAGGCCACAGATCGCCTCTTCAAAAAACTATGGCTCTCCATTGATCAGTG GTTCTACTCCAAAGCATGAACGGGGTTCTCCCAGCCACAGTAAGTCGCCAGCATATACACCTCAGAATGT GGACAGTGAAAGTGAGTCAGGCTCCTCCATAGCAGAGAGATCCTACCAGAACAGCCCCAGCTCAGATGAT GGTGTCCGGCCACTCCCAGAATACAGCACAGAGAAGCATAAGAAGCACAAAAAGGAAAAGAAAAAAGTAA AAGATAAAGATAGAGACCGGGACAGAGACAAAGACAGAGACAAGAAAAAATCTCACAGCATCAAGCCAGA GAATTGGTCTAAATCCCCCATTTCTTCAGATCAGACTTCATCTATGACAAATAACCCTATCTTATCTGCA GACAGGCCTTCCAGGCTCAGCCCTGACTTTATGATTGGGGAGGAAGATGATGATCTTATGGATGTGGCTC TGATTGGCAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209792 representing NM_001134361
Red=Cloning site Green=Tags(s) MSSLLDRLHAKFNQSRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIA RQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLK GLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNATPLDKILHGSVGYLTPRSGGHLMNMKYY ASPSDLLHDTTASPITLHEKNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPADNKWTPSFSAVTSAN SVDLPACFFLKFPQPIPVSKAFVQKLQNCTGIPLFETPPTYLPLYELITQFELSKDPDPLPLNHNMRFYA ALPGQQHCYFLNKDAPLPDGQSLQGTLVSKITFQHPGRVPLILNMIRHQVAYNTLIGSCVKRTILKEDSP GLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIP VTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGSNPMSGTTTPTNTFPGGPITTL FNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPML MNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKNKSSRVPPDKPKHQTEDDFQRELFSM DVDSQNPMFDVSMTADALDTPHITPAPSQCSTPPTTYPQPVSHPQPSIQRMGRLSSSDSIGPDVTDILSD IAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPT NHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGMPMLGGDNGESKLKGSSQ ADTVDFSILSVAGKALGAADLLEHHSGSQSPLLTTGELGKEKTQKRVKEGNGMGASSGSGPGSDSKPGKR SRTPSNDGKSKDKPPKRKKADTEGKSPSHSSNRPFTPPTSTGGSKSPGSSGRCQTPPGVATPPIPKITIQ IPKGTVMVGKPSSHSQYSSSGSVSSSGSKSHHSHSSSSSSLSSASTSGKMKSSKSEGSSSAKLSGSMYSS QGSSGSSQSKNSSQTGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSISKPNISPSHSRPPGGSDK LASPMKPVPGTPPSSKAKSPISSGSGSSHVSGTSSSSGMKSSSGSGSSGSLSQKTPPASNSCTPSSSSFS SSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPIDSQMGASTNSSNHPMSSKH NMSGGEFQSKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPG DSGGEGLRPQIASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNVDSESESGSSIAERSYQNSPSSDD GVRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPENWSKSPISSDQTSSMTNNPILSA DRPSRLSPDFMIGEEDDDLMDVALIGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001134361 |
ORF Size | 4701 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001134361.1, NP_001127833.1 |
RefSeq Size | 5813 bp |
RefSeq ORF | 4704 bp |
Locus ID | 497991 |
MW | 166.4 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209792 | Med1 (untagged ORF) - Rat mediator complex subunit 1 (Med1), (10 ug) |
CNY 25,180.00 |