Cldn15 (NM_001107135) Rat Untagged Clone

CAT#: RN202405

Cldn15 (untagged ORF) - Rat claudin 15 (Cldn15), (10 ug)



  "NM_001107135" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN202405 representing NM_001107135
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGATAGCTGTGGAGACCTTTGGTTTCTTCATGTCAGCCCTGGGACTGCTGATGCTGGGGGTGACCC
TTCCAAACAGCTACTGGAGAGTGTCTACCGTCCATGGGAACGTCATCACCACTAACACCATCTTCGAGAA
CCTGTGGTACAGCTGTGCCACCGACTCCCTGGGAGTCTCCAACTGCTGGGACTTCCCGTCCATGCTGGCC
CTCTCTGGCTATGTCCAGGGCTGCCGAGCTCTCATGATCACCGCCATCCTCCTGGGCTTCCTGGGCCTCT
TTCTAGGCATGGTGGGGCTCCGCTGCACCAACGTGGGCAACATCGACCTCTCCAGAAAGGCCAAGCTGCT
GGCCATTGCAGGGGCCTTCCACATACTTGCTGGAGCCTGTGGCATGGTTGCTATCTCATGGTATGCTGTC
AACATCACCACCGACTTCTTCAACCCCCTGTATGTTGGAACCAAGTATGAACTGGGCTCTGCCCTCTACT
TGGGCTGGAGCGCCTCTCTGCTCTCCATCCTGGGCGGCATCTGTGTCTTCTCCACCTGCTGCTGTGACTC
CAAGGAGGACCCAGCCACCAGGGTGGGACTTCCCTACAAGCCTTCTACGGTTGTGACAGCCCGAGCCACT
TCCGATGAAAGTGACGTCAGCTTTGGTAAATATGGCAAAAATGCCTACGTGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001107135
Insert Size 684 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001107135.2, NP_001100605.1
RefSeq Size 684 bp
RefSeq ORF 684 bp
Locus ID 304388
UniProt ID D3ZQJ0
Gene Summary Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members function as impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN15 forms tight junctions that mediate the paracellular transport of small monovalent cations along a concentration gradient, due to selective permeability for Na(+), Li(+) and K(+) ions, but selects against Cl(-) ions. Plays an important role in paracellular Na(+) transport in the intestine and in Na(+) homeostasis. Required for normal Na(+)-dependent intestinal nutrient uptake (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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