Cldn15 (NM_001107135) Rat Tagged Lenti ORF Clone
CAT#: RR202405L3
- LentiORF®
Lenti ORF clone of Cldn15 (Myc-DDK-tagged ORF) - Rat claudin 15 (Cldn15), (10 ug)
"NM_001107135" in other vectors (3)
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CNY 6,080.00
CNY 600.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RR202405).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001107135 |
ORF Size | 681 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001107135.2, NP_001100605.1 |
RefSeq Size | 684 bp |
RefSeq ORF | 684 bp |
Locus ID | 304388 |
UniProt ID | D3ZQJ0 |
Gene Summary | Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members function as impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN15 forms tight junctions that mediate the paracellular transport of small monovalent cations along a concentration gradient, due to selective permeability for Na(+), Li(+) and K(+) ions, but selects against Cl(-) ions. Plays an important role in paracellular Na(+) transport in the intestine and in Na(+) homeostasis. Required for normal Na(+)-dependent intestinal nutrient uptake (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN202405 | Cldn15 (untagged ORF) - Rat claudin 15 (Cldn15), (10 ug) |
CNY 3,990.00 |
|
RR202405 | Cldn15 (Myc-DDK-tagged ORF) - Rat claudin 15 (Cldn15), (10 ug) |
CNY 3,990.00 |
|
RR202405L4 | Lenti ORF clone of Cldn15 (mGFP-tagged ORF) - Rat claudin 15 (Cldn15), (10 ug) |
CNY 6,650.00 |